Culture medium composition adapting for blueberry test tube plantlet proliferation as well as method thereof

A technology of proliferation medium and culture medium, which is applied in the field of plant tissue culture, can solve the problems of rising tissue culture costs and the inability to realize large-scale industrial production, and achieve the effects of saving tissue culture costs, good applicability, and high proliferation coefficient

Inactive Publication Date: 2008-05-14
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the existing literature reports use expensive zeatin for multiplication, which ...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: This medium composition suitable for blueberry test-tube seedling propagation in this example 1, the medium includes the basic medium and the components of each stage of tissue culture medium and the weight contained in each liter is:

[0028] (1) Basic medium: WPM, in which white sugar 30g / L, agar 7-8g / L, pH 5.3;

[0029] (2) Induction medium: WPM+TDZ1.0mg / L+IBA0.3mg / L;

[0030] (3) Proliferation medium: WPM+TDZ0.2mg / L+IBA0.1mg / L+GA0.1mg / L.

[0031] This tissue culture method suitable for the proliferation of blueberry test-tube seedlings is carried out according to the following steps:

[0032] 1), the preparation of substratum, including each component of basic substratum and each stage substratum of tissue culture and the contained weight per liter are:

[0033] (1) Basic medium: WPM, in which white sugar 30g / L, agar 7-8g / L, pH 5.3;

[0034] (2) Induction medium: WPM+TDZ1.0mg / L+IBA0.3mg / L;

[0035] (3) Proliferation medium: WPM+TDZ0.2mg / L+IBA0.1mg / ...

Embodiment 2

[0040] Embodiment 2: This medium composition suitable for the proliferation of blueberry test-tube plantlets in this example 2, the medium includes the basic medium and each component of the medium at each stage of tissue culture and the weight contained in each liter is:

[0041] (1) Basic medium: WPM, in which white sugar 30g / L, agar 7-8g / L, pH 5.3;

[0042] (2) Induction medium: WPM+TDZ2mg / L+IBA0.5mg / L;

[0043] (3) Proliferation medium: WPM+TDZ0.5mg / L+IBA0.2mg / L+GA0.1mg / L.

[0044] This tissue culture method suitable for the proliferation of blueberry test-tube seedlings is carried out according to the following steps:

[0045] 1), the preparation of substratum, including each component of basic substratum and each stage substratum of tissue culture and the contained weight per liter are:

[0046] (1) Basic medium: WPM, in which white sugar 30g / L, agar 7-8g / L, pH 5.3;

[0047] (2) Induction medium: WPM+TDZ2mg / L+IBA0.5mg / L;

[0048] (3) Proliferation medium: WPM+TDZ0.5m...

Embodiment 3

[0053] Embodiment 3: This medium composition suitable for the proliferation of blueberry test-tube plantlets in this example 3, the medium includes the basic medium and each component of the medium at each stage of tissue culture and the weight contained in each liter is:

[0054] (1) Basic medium: WPM, in which white sugar 30g / L, agar 7-8g / L, pH 5.3;

[0055] (2) Leaf induction medium: WPM+TDZ 2.0 mg / L;

[0056] (3) Proliferation medium: WPM+TDZ0.2mg / L+IBA0.1 / L+GA0.1mg / L.

[0057] This tissue culture method suitable for the proliferation of blueberry test-tube seedlings is carried out according to the following steps:

[0058] 1), the preparation of substratum, including each component of basic substratum and each stage substratum of tissue culture and the contained weight per liter are:

[0059] (1) Basic medium: WPM, in which white sugar 30g / L, agar 7-8g / L, pH 5.3;

[0060] (2) Leaf induction medium: WPM+TDZ 2.0 mg / L;

[0061] (3) Proliferation medium: WPM+TDZ0.2mg / L+IB...

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Abstract

The present invention relates to a kind of culture medium composition suitable for blueberry test-tube seedling proliferation and method thereof, the steps are as follows: 1) culture medium comprises basic culture medium and each component of culture medium of each stage of tissue culture and the contained weight per liter is: ( 1) Basic medium: WPM, including sugar 30g/L, agar 7~8g/L, pH5.3; (2) Induction medium: WPM+TDZ1.0~2.0mg/L+IBA0.3~0.5mg/L L; (3) leaf induction medium: WPM+TDZ2.0~3.0mg/L; (4) proliferation medium: WPM+TDZ0.2~0.5mg/L+IBA0.1~0.2mg/L+GA0. 1mg/L. 2) Selection and sterilization of explants; 3) Induction culture: directly induce the primary shoots from the explants; 4) Culture on the leaf induction medium to form embryogenic callus or directly form a complete plant Regeneration of adventitious buds; 5) Proliferation culture. The beneficial effects of the invention are as follows: 1) the culture medium has strong pertinence and good applicability, and the bud induction rate is high; 2) the multiplication coefficient is high, and the tissue culture cost is saved. 3) The genetic traits are consistent, which overcomes the disadvantage of low conventional reproduction coefficient.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a medium composition suitable for the proliferation of blueberry test-tube seedlings and a method thereof. Background technique [0002] Blueberry (blueberry) is an emerging functional fruit. It has quickly become the third largest berry in the world due to its excellent quality, excellent processing performance and unique health care function, and is listed as one of the five major healthy foods for human beings by the International Food and Agriculture Organization. [0003] Blueberry fruit is low in energy and contains a variety of antioxidant components, so it not only has the peculiar effect of anti-cancer and anti-cancer, but also can significantly reduce the incidence of cardiovascular disease, diabetes, Perkins disease, etc. It is also an important anti-aging and weight loss. Beauty Fruit. Blueberry is suitable for processing into...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N5/04
Inventor 张慧琴谢鸣蒋桂华孙崇波吴延军梁英龙
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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