Tissue culture and rapid propagation method of eucalyptus urophylla DH33 to DH27 varieties

A technology of tissue culture rapid propagation and varieties, applied in horticultural methods, botanical equipment and methods, climate change adaptation, etc., can solve the problems of inability to effectively inherit the excellent characteristics of parents, tissue culture work has not been carried out effectively, and excellent characteristics need to be studied. , to achieve the effects of improving economic and social benefits, being easy to promote and use, and reducing the risk of tissue culture

Active Publication Date: 2015-01-14
广西壮族自治区国有东门林场
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  • Claims
  • Application Information

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Problems solved by technology

The above-mentioned patent uses the stem section of Eucalyptus urophylla aseptic seedlings as explants to induce embryogenic callus, and then obtains regenerated plants through the regeneration of somatic embryogenesis. The operation is relatively complicated and difficult to implement. The stems of the seedlings are explants, and the excellent characteristics of the female parent need to be studied. The commercial seedling cultivation using this technology alone cannot effectively inherit the excellent characteristics of the parents.
[0009] It can be seen that although there are many studies on the tissue culture technology of eucalyptus at present, there is still a lot of work to be

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  • Tissue culture and rapid propagation method of eucalyptus urophylla DH33 to DH27 varieties

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Embodiment 1

[0032] The tissue culture rapid propagation method of the Eucalyptus macrocephalus DH33-27 variety, the specific steps are as follows:

[0033] ①Establishment of explants: The selected DH33-27 variety was planted into a test forest. In February, a 4-5-year-old tree with a strong growth, a thick trunk, and no pests and diseases was selected. Fall at -25 centimeters. After one month, the stump begins to grow buds. After 2 weeks, when the buds are about 10 centimeters long, semi-lignified, and the bud eyes have not germinated, the buds are picked back as explant materials. In order to reduce the source of material pollution, when taking bud strips, it should be carried out after more than three consecutive days of fine weather. After removing most of the leaves from the harvested bud strips, rinse them with tap water for 15 minutes, soak them in 0.5% washing powder solution for 15 minutes, and then rinse them with tap water.

[0034] ② Disinfection of explants: step ① Soak the o...

Embodiment 2

[0041] The tissue culture rapid propagation method of the Eucalyptus macrocephalus DH33-27 variety, the specific steps are as follows:

[0042] ①Establishment of explants: The selected DH33-27 variety was planted into a test forest. In February, a 4-5-year-old tree with a strong growth, a thick trunk, and no pests and diseases was selected. Fall at -25 centimeters. After one month, the stump begins to grow buds. After 2 weeks, when the buds are about 10 centimeters long, semi-lignified, and the bud eyes have not germinated, the buds are picked back as explant materials. In order to reduce the source of material pollution, when taking bud strips, it should be carried out after more than three consecutive days of fine weather. After removing most of the leaves from the harvested bud strips, rinse them with tap water for 15 minutes, soak them in 0.5% washing powder solution for 15 minutes, and then rinse them with tap water.

[0043] ② Disinfection of explants: step ① Soak the o...

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Abstract

The invention discloses a tissue culture and rapid propagation method of eucalyptus urophylla DH33 to DH27 varieties. The method comprises the following steps: establishing an explant, sterilizing the explant, carrying out primary culture, carrying out secondary culture, carrying out rooting culture, exercising seedlings, transplanting and the like. A special culture medium is selected for the primary culture, the secondary culture and the rooting culture, the eucalyptus urophylla can grow fast and is high in survival rate due to the reasonable seedling exercising means, and the purposes of high propagation coefficient and good propagation effect can be achieved. Meanwhile, the method is low in production cost, the genetic traits of the obtained tissue-culture seedlings are consistent, the method is an effective way for the tissue culture and rapid propagation of the eucalyptus, and the application range is wide.

Description

technical field [0001] The invention relates to the technical field of eucalyptus cultivation, in particular to a method for tissue culture and rapid propagation of Eucalyptus macrocephalus DH33-27 variety. Background technique [0002] Eucalyptus grandis DH33-27 variety (E .urophylla×E . Grandis) , is Guangxi Dongmen Forest Farm, with Indonesian provenance Eucalyptus urophylla U16 (E ucalyptus urophylla ) as the female parent, and the Australian provenance Eucalyptus grandis G33 (E ucalyptus grandis ) as the male parent hybridization dominant species, which is an excellent eucalyptus variety obtained through asexual propagation. Peel off completely. At the same time, it has excellent comprehensive performance, straight trunk shape, neat forest appearance, small branches, no pests and diseases, large annual average storage volume, obvious economic benefits, and is suitable for large-scale promotion and planting. [0003] Years of popularization tests have proved that...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCY02A40/22Y02P60/40
Inventor 陈德文苏勇沈云庞贞武黄全东莫继有吴兵梁秀莉陈允椿韦炳俭唐再生吴满芬俸荣娣邓冬丽付淼吕华丽兰俊石前陈东林李丽芳熊涛王建忠张磊龚卫新
Owner 广西壮族自治区国有东门林场
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