A kind of tissue culture rapid propagation method of Eucalyptus urophylla dh32-43 variety

A technology of tissue culture rapid propagation and varieties, applied in horticultural methods, botanical equipment and methods, climate change adaptation, etc., can solve the problems of inability to effectively inherit the excellent characteristics of parents, tissue culture work has not been carried out effectively, and excellent characteristics need to be studied. , to achieve the effects of improving economic and social benefits, being easy to promote and use, and reducing the risk of tissue culture

Active Publication Date: 2016-04-06
广西壮族自治区国有东门林场
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  • Abstract
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Problems solved by technology

The above-mentioned patent uses the stem section of Eucalyptus urophylla aseptic seedlings as explants to induce embryogenic callus, and then obtains regenerated plants through the regeneration of somatic embryogenesis. The operation is relatively complicated and difficult to implement. The stems of the seedlings are explants, and the excellent characteristics of the female parent need to be studied. The commercial seedling cultivation using this technology alone cannot effectively inherit the excellent characteristics of the parents.
[0009] It can be seen that although there are many studies on the tissue culture technology of eucalyptus at present, there is still a lot of work to be explored for the purposeful multiplication of the excellent traits of the excellent trees, and the tissue culture and rapid propagation methods that can be effectively popularized and applied. Because DH32-43 It is a perennial species of Eucalyptus urophylla. There are few tissue-cultured seedlings in the market and resources are in short supply. However, the tissue culture of this variety has not been effectively carried out. The tissue culture research on the new variety of Eucalyptus urophylla DH32-43 is helpful for the rapid development of this excellent variety. It is of great significance to breed, meet the market supply, and improve the ecological and economic benefits of this fine variety

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  • A kind of tissue culture rapid propagation method of Eucalyptus urophylla dh32-43 variety

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Effect test

Embodiment 1

[0032] The tissue culture rapid propagation method of the Eucalyptus macrophylla DH32-43 kind, concrete steps are as follows:

[0033] ①Establishment of explants: Select 4-5 years old trees with strong growth, thick trunks, and no pests and diseases, and cut them down from a place 20-25cm above the ground. When sprouting, the bud strips were taken back as explant material. In order to reduce the source of material pollution, when taking bud strips, it should be carried out after more than three consecutive days of fine weather. After removing most of the leaves from the harvested bud strips, rinse them with tap water for 15 minutes, soak them in 0.5% washing powder solution for 15 minutes, and then rinse them with tap water.

[0034] ② Disinfection of explants: step ① Soak the obtained explants in 75% alcohol for 30 seconds, rinse them with sterile water for 5 times, drain the water, put them in 0.1% mercuric chloride solution for disinfection for 9 minutes, and rinse them wi...

Embodiment 2

[0041] The tissue culture rapid propagation method of the Eucalyptus macrophylla DH32-43 kind, concrete steps are as follows:

[0042] ①Establishment of explants: Select 4-5 years old trees with strong growth, thick trunks, and no pests and diseases, and cut them down from a place 20-25cm above the ground. When sprouting, the bud strips were taken back as explant material. In order to reduce the source of material pollution, when taking bud strips, it should be carried out after more than three consecutive days of fine weather. After removing most of the leaves from the harvested bud strips, rinse them with tap water for 15 minutes, soak them in 0.5% washing powder solution for 15 minutes, and then rinse them with tap water.

[0043] ② Disinfection of explants: step ① Soak the obtained explants in 75% alcohol for 30 seconds, rinse them with sterile water for 4 times, drain the water, put them in 0.1% mercuric chloride solution for disinfection for 9 minutes, and rinse them wi...

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Abstract

The invention discloses a tissue culture rapid propagation method of Eucalyptus urophylla DH32-43 variety, which is realized through the steps of explant establishment, explant treatment, primary culture, subculture, rooting culture, seedling hardening and transplanting. , A special medium composition was selected for primary culture, subculture and rooting culture. Reasonable seedling hardening measures made Eucalyptus macrophylla grow fast after transplanting, with a high survival rate, and achieved the purpose of high reproduction coefficient and good reproduction effect. Simultaneously, the production cost of the method of the invention is low, and the obtained tissue culture seedlings have consistent genetic traits, which is an effective way for tissue culture and rapid propagation of eucalyptus, and has a wide application range.

Description

technical field [0001] The invention relates to the technical field of eucalyptus cultivation, in particular to a method for tissue culture and rapid propagation of Eucalyptus macrocephalus DH32-43 variety. Background technique [0002] Eucalyptus (Eucalyptus) is the general name of Myrtaceae (Myrtaceae) Eucalyptus (Eucalyptus) plants. It is native to Australia. There are more than 100 species, of which more than 40 are widely cultivated. In recent years, all parts of our country are vigorously developing eucalyptus planting, forming a certain scale in Guangdong, Guangxi, Hainan, Sichuan, Yunnan and other provinces, which has produced obvious ecological and economic benefits. [0003] Variety of Eucalyptus grandis DH32-43 ( E. urophylla × E. Grandis) , is Guangxi Dongmen Forest Farm, with Indonesian provenance Eucalyptus urophylla U16 ( Eucalyptus urophylla ) as the female parent, and the Australian provenance Eucalyptus grandis G46 ( Eucalyptus grandis ) is an excelle...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCY02A40/22Y02P60/40
Inventor 沈云莫继有兰俊陈德文苏勇庞贞武梁秀莉黄全东吴兵陈允椿梁庭辉唐再生韦炳俭吴满芬俸荣娣邓冬丽付淼吕华丽石前陈东林李丽芳熊涛王建忠张磊
Owner 广西壮族自治区国有东门林场
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