A method for tissue culture and rapid propagation of Eucalyptus urophylla dh30-1 variety
A technology for tissue culture, rapid propagation and variety, applied in horticultural methods, botanical equipment and methods, climate change adaptation, etc., can solve problems such as the inability to effectively inherit the excellent characteristics of the parents, the tissue culture work has not been carried out effectively, and the excellent characteristics need to be studied. , to improve economic and social benefits, reduce tissue culture risks, and inhibit bacterial infection
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Embodiment 1
[0032] The tissue culture rapid propagation method of Eucalyptus macrocephalus DH30-1 variety, the specific steps are as follows:
[0033] ①Establishment of explants: The selected DH30-1 variety was planted into a test forest. In February, a 4-5-year-old tree with strong growth, thick trunk, and no pests and diseases was selected. Fall at -25 centimeters. After one month, the stump begins to grow buds. After 2 weeks, when the buds are about 10 centimeters long, semi-lignified, and the bud eyes have not germinated, the buds are picked back as explant materials. In order to reduce the source of material pollution, when taking bud strips, it should be carried out after more than three consecutive days of fine weather. After removing most of the leaves from the harvested bud strips, rinse them with tap water for 15 minutes, soak them in 0.5% washing powder solution for 15 minutes, and then rinse them with tap water.
[0034] ② Disinfection of explants: step ① Soak the obtained ex...
Embodiment 2
[0041] The tissue culture rapid propagation method of Eucalyptus macrocephalus DH30-1 variety, the specific steps are as follows:
[0042] ①Establishment of explants: The selected DH30-1 variety was planted into a test forest. In February, a 4-5-year-old tree with strong growth, thick trunk, and no pests and diseases was selected. Fall at -25 centimeters. After one month, the stump begins to grow buds. After 2 weeks, when the buds are about 10 centimeters long, semi-lignified, and the bud eyes have not germinated, the buds are picked back as explant materials. In order to reduce the source of material pollution, when taking bud strips, it should be carried out after more than three consecutive days of fine weather. After removing most of the leaves from the harvested bud strips, rinse them with tap water for 15 minutes, soak them in 0.5% washing powder solution for 15 minutes, and then rinse them with tap water.
[0043] ② Disinfection of explants: step ① Soak the obtained ex...
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