A method for tissue culture and rapid propagation of Eucalyptus urophylla dh30-1 variety

Abstract

The invention discloses a tissue culture and rapid propagation method of Eucalyptus urophylla DH30-1 variety, which is realized through the steps of establishment of explants, disinfection of explants, primary culture, subculture, rooting culture, seedling hardening and transplanting. , A special medium composition was selected for primary culture, subculture and rooting culture. Reasonable seedling hardening measures made Eucalyptus macrophylla grow fast after transplanting, with a high survival rate, and achieved the purpose of high reproduction coefficient and good reproduction effect. Simultaneously, the production cost of the method of the invention is low, and the obtained tissue culture seedlings have consistent genetic traits, which is an effective way for tissue culture and rapid propagation of eucalyptus, and has a wide application range.

Description

technical field

[0001] The invention relates to the technical field of eucalyptus cultivation, in particular to a method for tissue culture and rapid propagation of Eucalyptus urophylla DH30-1 variety. Background technique

[0002] Eucalyptus grandis DH30-1 variety (E .urophylla×E . Grandis) , is Guangxi Dongmen Forest Farm, with Indonesian provenance Eucalyptus urophylla U16 (E ucalyptus urophylla ) as the female parent, and the Australian provenance Eucalyptus grandis G22 (E ucalyptus grandis ) as the male parent hybrid dominant species, the excellent eucalyptus species obtained through vegetative propagation, has excellent comprehensive performance, straight trunk shape, neat forest appearance, small branches, no pests and diseases, large annual accumulation volume, obvious economic benefits, suitable for large areas Widespread planting.

[0003] Years of popularization tests have proved that the 6.5-year-old DH30-1 is the most outstanding. In the Duncan test of th...

Examples

Embodiment 1

[0032] The tissue culture rapid propagation method of Eucalyptus macrocephalus DH30-1 variety, the specific steps are as follows:

[0033] ①Establishment of explants: The selected DH30-1 variety was planted into a test forest. In February, a 4-5-year-old tree with strong growth, thick trunk, and no pests and diseases was selected. Fall at -25 centimeters. After one month, the stump begins to grow buds. After 2 weeks, when the buds are about 10 centimeters long, semi-lignified, and the bud eyes have not germinated, the buds are picked back as explant materials. In order to reduce the source of material pollution, when taking bud strips, it should be carried out after more than three consecutive days of fine weather. After removing most of the leaves from the harvested bud strips, rinse them with tap water for 15 minutes, soak them in 0.5% washing powder solution for 15 minutes, and then rinse them with tap water.

[0034] ② Disinfection of explants: step ① Soak the obtained ex...

Embodiment 2

[0041] The tissue culture rapid propagation method of Eucalyptus macrocephalus DH30-1 variety, the specific steps are as follows:

[0042] ①Establishment of explants: The selected DH30-1 variety was planted into a test forest. In February, a 4-5-year-old tree with strong growth, thick trunk, and no pests and diseases was selected. Fall at -25 centimeters. After one month, the stump begins to grow buds. After 2 weeks, when the buds are about 10 centimeters long, semi-lignified, and the bud eyes have not germinated, the buds are picked back as explant materials. In order to reduce the source of material pollution, when taking bud strips, it should be carried out after more than three consecutive days of fine weather. After removing most of the leaves from the harvested bud strips, rinse them with tap water for 15 minutes, soak them in 0.5% washing powder solution for 15 minutes, and then rinse them with tap water.

[0043] ② Disinfection of explants: step ① Soak the obtained ex...

technical field

[0001] The invention relates to the technical field of eucalyptus cultivation, in particular to a method for tissue culture and rapid propagation of Eucalyptus urophylla DH30-1 variety. Background technique

[0002] Eucalyptus grandis DH30-1 variety (E .urophylla×E . Grandis) , is Guangxi Dongmen Forest Farm, with Indonesian provenance Eucalyptus urophylla U16 (E ucalyptus urophylla ) as the female parent, and the Australian provenance Eucalyptus grandis G22 (E ucalyptus grandis ) as the male parent hybrid dominant species, the excellent eucalyptus species obtained through vegetative propagation, has excellent comprehensive performance, straight trunk shape, neat forest appearance, small branches, no pests and diseases, large annual accumulation volume, obvious economic benefits, suitable for large areas Widespread planting.

[0003] Years of popularization tests have proved that the 6.5-year-old DH30-1 is the most outstanding. In the Duncan test of th...

Examples

Embodiment 1

[0032] The tissue culture rapid propagation method of Eucalyptus macrocephalus DH30-1 variety, the specific steps are as follows:

[0033] ①Establishment of explants: The selected DH30-1 variety was planted into a test forest. In February, a 4-5-year-old tree with strong growth, thick trunk, and no pests and diseases was selected. Fall at -25 centimeters. After one month, the stump begins to grow buds. After 2 weeks, when the buds are about 10 centimeters long, semi-lignified, and the bud eyes have not germinated, the buds are picked back as explant materials. In order to reduce the source of material pollution, when taking bud strips, it should be carried out after more than three consecutive days of fine weather. After removing most of the leaves from the harvested bud strips, rinse them with tap water for 15 minutes, soak them in 0.5% washing powder solution for 15 minutes, and then rinse them with tap water.

[0034] ② Disinfection of explants: step ① Soak the obtained ex...

Embodiment 2

[0041] The tissue culture rapid propagation method of Eucalyptus macrocephalus DH30-1 variety, the specific steps are as follows:

[0042] ①Establishment of explants: The selected DH30-1 variety was planted into a test forest. In February, a 4-5-year-old tree with strong growth, thick trunk, and no pests and diseases was selected. Fall at -25 centimeters. After one month, the stump begins to grow buds. After 2 weeks, when the buds are about 10 centimeters long, semi-lignified, and the bud eyes have not germinated, the buds are picked back as explant materials. In order to reduce the source of material pollution, when taking bud strips, it should be carried out after more than three consecutive days of fine weather. After removing most of the leaves from the harvested bud strips, rinse them with tap water for 15 minutes, soak them in 0.5% washing powder solution for 15 minutes, and then rinse them with tap water.

[0043] ② Disinfection of explants: step ① Soak the obtained ex...

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