Simple Tissue Culture Reproduction Technology of Acer palmatum Qinglong
A technology of Acer japonica and Qinglong, applied in the field of plant tissue culture, can solve the problems such as the Qinglong tissue culture propagation method that has not been reported, and achieves the effects of low production cost, simple tissue culture reproduction technology, and short reproduction cycle.
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Embodiment 1
[0020] The simple tissue culture propagation technique of Acer palmatum Qinglong comprises the following steps:
[0021] S1. Collect disease-free branches of Acer palmatum Qinglong in the same year at noon or afternoon on a sunny day, remove excess leaves, and cut into stems with buds. The stems with buds are first sterilized with alcohol for 30 seconds, then sterilized with mercury chloride for 5 minutes, and then Wash 4-6 times with sterile water;
[0022] S2, inoculate the sterilized stem section with buds on the medium to grow, as figure 1 As shown, the medium is MS+NAA 0.3mg / L, and the inoculated culture material is placed in a culture room with a light intensity of 2000-3000LX, a light time of 16h / d, and a temperature of 24-26°C. , cultivated for 15 days to induce germination of axillary buds;
[0023] S3, rooting culture, cutting directly induced axillary buds, inoculated on the rooting medium, such as figure 2 As shown, the rooting medium is 1 / 2MS+NAA0.3mg / L+IAA 0....
Embodiment 2
[0025] The simple tissue culture propagation technique of Acer palmatum Qinglong comprises the following steps:
[0026] S1. Collect disease-free branches of Acer palmatum Qinglong in the same year at noon or afternoon on a sunny day, remove excess leaves, and cut into stems with buds. The stems with buds are first sterilized with alcohol for 50 seconds, then sterilized with mercury chloride for 4 minutes, and then Wash 4-6 times with sterile water;
[0027] S2, inoculate the sterilized stem section with buds on the medium to grow, as figure 1 As shown, the medium is MS+NAA 0.3mg / L, and the inoculated culture material is placed in a culture room with a light intensity of 2000-3000LX, a light time of 16h / d, and a temperature of 24-26°C. , cultivated for 2 weeks to induce axillary bud germination;
[0028] S3, rooting culture, cutting directly induced axillary buds, inoculated on the rooting medium, such as figure 2 As shown, the rooting medium is 1 / 2MS+NAA0.3mg / L+IAA 0.2 mg...
Embodiment 3
[0030] The simple tissue culture propagation technique of Acer palmatum Qinglong comprises the following steps:
[0031] S1. Collect disease-free branches of Acer palmatum Qinglong at noon or afternoon on a sunny day, remove excess leaves, and cut into stems with buds. The stems with buds are first sterilized with alcohol for 60 seconds, then sterilized with mercury chloride for 3 minutes, and then Wash 4-6 times with sterile water;
[0032] S2, inoculate the sterilized stem section with buds on the medium to grow, as figure 1 As shown, the medium is MS+NAA 0.3mg / L, and the inoculated culture material is placed in a culture room with a light intensity of 2000-3000LX, a light time of 16h / d, and a temperature of 24-26°C. , cultivated for 3 weeks to induce axillary bud germination;
[0033] S3, rooting culture, cutting directly induced axillary buds, inoculated on the rooting medium, such as figure 2 As shown, the rooting medium is 1 / 2MS+NAA0.3mg / L+IAA 0.2 mg / L, and the inocu...
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