Tissue culture method of mytilaria laosensis

A tissue culture and rice row technology, which is applied in the field of plant tissue culture, can solve the problems of large differentiation of seedlings and difficulty in obtaining maximum yield for artificial forests.

Inactive Publication Date: 2021-05-28
龙岩市禾康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The Milaopai plantation in China all adopts seedling cultivation for afforestation, and th...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The tissue culture method of rice Lao Pai in present embodiment 1 comprises the following steps:

[0030] S1. Gather the 25-30cm sprouted strips of Mi Lao Pai in the same year, subtract the leaves on the sprouted strips, and cut it into 4-5cm stem sections, and each stem section has 1-2 axillary buds. The stem section with axillary buds is immersed in 70-75% alcohol for 10 seconds to be disinfected. After taking it out, cut off 1-1.5 cm from both ends of the stem section without cutting off the axillary buds, leaving the middle section as a sterilized explant;

[0031] S2. Inoculate the sterilized explants in the proliferation medium in the incubator, control the temperature in the incubator to be 27° C., and the humidity to be 80%. First use red light to irradiate and cultivate for 3 days, then blue light to irradiate and cultivate for 3 days, and finally Use green light to irradiate and cultivate for 3 days, take red light, blue light and green light to irradiate and ...

Embodiment 2

[0035] The tissue culture method of rice Lao Pai in present embodiment 2 comprises the following steps:

[0036]S1. Gather the 25-30cm sprouted strips of Mi Lao Pai in the same year, subtract the leaves on the sprouted strips, and cut it into 4-5cm stem sections, and each stem section has 1-2 axillary buds. The stem section with axillary buds is immersed in 70-75% alcohol for 10 seconds to be disinfected. After taking it out, cut off 1-1.5 cm from both ends of the stem section without cutting off the axillary buds, leaving the middle section as a sterilized explant;

[0037] S2. Inoculate the sterilized explants in the proliferation medium in the incubator, control the temperature in the incubator to be 29° C., and the humidity to be 85%. First use red light to irradiate and cultivate for 5 days, then blue light to irradiate and cultivate for 5 days, and finally Use green light to irradiate and cultivate for 5 days, take red light, blue light and green light to irradiate and c...

Embodiment 3

[0041] The tissue culture method of rice Lao Pai in present embodiment 3 comprises the following steps:

[0042] S1. Gather the 25-30cm sprouted strips of Mi Lao Pai in the same year, subtract the leaves on the sprouted strips, and cut it into 4-5cm stem sections, and each stem section has 1-2 axillary buds. The stem section with axillary buds is immersed in 70-75% alcohol for 10 seconds to be disinfected. After taking it out, cut off 1-1.5 cm from both ends of the stem section without cutting off the axillary buds, leaving the middle section as a sterilized explant;

[0043] S2. Inoculate the sterilized explants in the proliferation medium in the incubator, control the temperature in the incubator to be 28° C., and the humidity to be 83%. First use red light to irradiate and cultivate for 4 days, then blue light to irradiate and cultivate for 4 days, and finally Use green light to irradiate and cultivate for 4 days, take red light, blue light and green light to irradiate the ...

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Abstract

The invention relates to the technical field of plant tissue culture, and particularly discloses a tissue culture method of mytilaria laosensis. The tissue culture method of the mytilaria laosensis comprises the following steps: S1, collecting top tips of current-year coppice shoots of the mytilaria laosensis, cutting off leaves, cutting the top tips into stem segments with axillary buds, and disinfecting the stem segments to obtain sterilized explants; S2, inoculating the sterilized explants into a proliferation culture medium, controlling the culture conditions that the environment temperature is 27-29 DEG C and the humidity is 80-85%, and correspondingly using red light, blue light and green light to irradiate and culture; S3, transferring the sterilized explants into a strong seedling culture medium at the temperature of 25-27 DEG C, and cutting off proliferation buds on the sterilized explants to obtain the proliferation buds after white light irradiation culture; and S4, transferring the proliferation buds into a rooting culture medium at the temperature of 27-29 DEG C and with the humidity of 80-85%, correspondingly using the red light, the blue light and the green light for irradiation culture, and transferring rooted proliferation buds into a sunlight greenhouse for seedling hardening to obtain adult seedlings. According to the tissue culture method of the mytilaria laosensis, an asexual propagation technology is used for breeding themytilaria laosensis.

Description

technical field [0001] The present application relates to the technical field of plant tissue culture, more specifically, it relates to a tissue culture method of Milaopai. Background technique [0002] Mi Lao Pai (Mytilaria laosensis Lecomte), also known as shell vegetable fruit and triangular maple, belongs to the evergreen broad-leaved tree of Hamamelidaceae. The height of the tree is more than 30m, and the diameter at breast height reaches 100cm. Naturally distributed in my country's Guangdong, Guangxi and Yunnan and Vietnam, Laos and other places. [0003] After the 1980s, it was successfully introduced to Fujian, Jiangxi, Zhejiang and other places in my country. Milaopai is a rare native broad-leaved tree species in the southern subtropical region of my country. In 1974, Yunnan Province selected Milaopai as an excellent timber tree species with high economic value and promising development prospects in tropical Yunnan. [0004] With the increase of the planting perio...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 樊仲书樊钰书樊小燕张玉华
Owner 龙岩市禾康生物科技有限公司
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