Acer paimatum seed tissue culture method

A technology of tissue culture and Acer palmatum, which is applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of limited material collection by seasons, low cutting propagation coefficient, and long seedling cycle, etc., to overcome the limitations of seasons , easy to obtain materials, and easy to operate

Active Publication Date: 2013-11-27
SICHUAN COLORLINK CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows us to grow large numbers of high-quality plantlets that have unique traits such as improved yielding ability or resistance against pests. These new vegetables may be useful for creation of better crops with less strict growing conditions than traditional methods like crop rotation.

Problems solved by technology

This patented describes different methods used during vegetative growth stages on grassy areas such as valleysides and rainforest zones. These techniques aim to enhance the appearance and quality of these environments through creation of vascular networks called phyllodes, which help control pH levels within specific ranges while providing essential oxygen sources needed for photosynthesis. Additionally, they provide various healthcare values including antioxic properties, antiinflamatory activity, blood pressure reduction, stimulation of nerves, reduced inflammations, and improved immune function.

Method used

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  • Acer paimatum seed tissue culture method
  • Acer paimatum seed tissue culture method
  • Acer paimatum seed tissue culture method

Examples

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Embodiment 1

[0020] Example 1: A kind of Acer palmatum seed tissue culture method, it comprises the following steps:

[0021] S1. Seed storage: Collect mature seeds of Acer palmatum in October every year, mix the seeds with sand, the mixing volume is 1:3, the humidity of the sand is such that it will form a ball without dripping, and it will crack when loosened. Store in a refrigerator at 4°C;

[0022] S2. Seed disinfection: Take out the stored seeds, wash them in running water for 5 minutes, peel off the seed shells with a scalpel and tweezers in an ultra-clean workbench, treat them with 75% alcohol for 15 seconds, and then put them in 0.1% mercuric chloride for 5 minutes , washed with sterile water 4 times;

[0023] S3. Start culture: inoculate the sterilized seeds in the start medium for cultivation, wherein the start medium is 1 / 2MS+GA 3 0.5mg / L + 30g / L sucrose + 5g / L agar, the pH value is 6, the culture conditions are: light intensity 2800LX, light 14h, temperature 22°C, culture ...

Embodiment 2

[0026] Example 2: A kind of Acer palmatum seed tissue culture method, it comprises the following steps:

[0027] S1. Seed storage: Collect mature seeds of Acer palmatum in October every year, mix the seeds with sand, the mixing volume is 1:3, the humidity of the sand is such that it will form a ball without dripping, and it will crack when loosened. Store in a refrigerator at 4°C;

[0028] S2. Seed disinfection: Take out the stored seeds, rinse them in running water for 10 minutes, use a scalpel and tweezers to peel off the seed shells in an ultra-clean workbench, treat them with 75% alcohol for 20 seconds, and then put them in 0.1% mercuric chloride for 10 minutes , washed with sterile water 6 times;

[0029] S3. Start culture: inoculate the sterilized seeds in the start medium for cultivation, wherein the start medium is 1 / 2MS+GA 3 0.5mg / L + 30g / L sucrose + 5g / L agar, the pH value is 7, the culture conditions are: light intensity 3200LX, light 18h, temperature 26°C, cult...

Embodiment 3

[0032] Example 3: A kind of Acer palmatum seed tissue culture method, it comprises the following steps:

[0033] S1. Seed storage: Collect mature seeds of Acer palmatum in October every year, mix the seeds with sand, the mixing volume is 1:3, the humidity of the sand is such that it will form a ball without dripping, and it will crack when loosened. Store in a refrigerator at 4°C;

[0034] S2. Seed disinfection: take out the stored seeds, rinse them in running water for 8 minutes, use a scalpel and tweezers to peel off the seed shells in an ultra-clean workbench, treat them with 75% alcohol for 18 seconds, and then put them in 0.1% mercuric chloride for 7 minutes , washed 5 times with sterile water;

[0035] S3. Start culture: inoculate the sterilized seeds in the start medium for cultivation, wherein the start medium is 1 / 2MS+GA 3 0.5mg / L + sucrose 30g / L + agar 5g / L, the pH value is 7, the culture conditions are: light intensity 3000LX, light 15h, temperature 24°C, culture ...

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Abstract

The invention discloses an acer paimatum seed tissue culture method. The acer paimatum seed tissue culture method comprises the following steps: seed storage: ripe acer paimatum seeds are collected in October every year, mixed with sand, and placed and stored in a refrigerator at a temperature of 4 DEG C; seed sterilization: the stored seeds are taken out and flushed, the casings of the seeds are peeled off, and the peeled seeds are sterilized with alcohol and mercuric chloride; initiation culture: an initiation culture medium comprises 1/2MS, 30.5 mg/L of GA, 30g/L of cane sugar and 5g/L of agar, and the pH value of the initiation culture medium is 6-7; primary culture: budded seeds are inoculated to a primary culture medium for culture, the primary culture medium is provided with 1/2 MS, 30.5mg/L of GA, 0.05mg/L of NAA, 30g/L of cane sugar and 5g/L of agar, and the pH value of the primary culture medium is 6-7; subculture: after tissue culture seedlings subjected to the primary culture are cut, each section of the tissue culture seedling has 2-5 axillary buds and is transferred to a subculture medium for culture, the subculture medium is provided with 1/2MS, 0.1mg/L of TDZ, 0.05mg/L of KT, 30g/L of cane sugar and 5g/L of agar, and the pH value of the subculture medium is 6-7. The acer paimatum seed tissue culture method is simple in culture process and convenient to operate, and provides technique support for industrial acer paimatum seedling-raising.

Description

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Claims

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Application Information

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Owner SICHUAN COLORLINK CO LTD
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