Plant tissue culture method for thinly-skinned walnut

A thin-skinned walnut and tissue culture technology, applied in the directions of horticultural methods, botanical equipment and methods, cultivation, etc., can solve the problems of easy oxidative browning at the grafting interface, difficult rooting of cuttings, low grafting survival rate, etc., and achieves easy migration. The effect of planting survival, transplanting easy survival, and short cultivation period

Inactive Publication Date: 2011-04-20
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at present, except for grafting, it is almost impossible to use other methods for vegetative propagation. Cuttings are not easy to take root, and grafting is difficult to survive due to oxidative browning at the interface. The survival rate of grafting is very low, and the rootstock must be cultivated first, which is laborious

Method used

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  • Plant tissue culture method for thinly-skinned walnut
  • Plant tissue culture method for thinly-skinned walnut
  • Plant tissue culture method for thinly-skinned walnut

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] a. Cut Xinwen No. 185 field walnut branches, rinse them with tap water for 10 minutes, then cut the branches into 1cm stem segments, each stem segment contains an axillary bud, and then wash the stem segments with 75% alcohol on an ultra-clean workbench Surface disinfection for 10 seconds, 0.1% mercury liter sterilization for 1 minute, 15% hydrogen peroxide treatment for 10 minutes, and sterile water washing for 4 times;

[0034] b. Insert the treated stem segment into the starting induction solid medium DKW+TDZ 0.1mg / L+IBA 0.01mg / L, the temperature is 23±1°C, the light is 2500 lx, the time is 16h / d, and the induction culture is carried out for 20 days;

[0035] c. Cut the induced new long axillary buds into stems with one axillary bud and put them into solid medium DKW+6-BA 0.1mg / L+IBA 0.001mg / L, place at a temperature of 23±1°C, and light 3000lx , time 16h / d for cultivation;

[0036] d. Cut out the 1.0cm-grown seedlings, insert them into solid medium 1 / 8DKW+IBA 1mg / ...

Embodiment 2

[0040] a. Cut the branch of Xinxin No. 2 field walnut, wash it with running water for 30 minutes, then cut the branch into 1cm stem section, each stem section contains an axillary bud, and then put the stem section on the ultra-clean workbench with 75% alcohol Surface disinfection for 30 seconds, 0.1% mercury liter sterilization for 5 minutes, 15% hydrogen peroxide treatment for 14 minutes, and sterile water washing for 4 times;

[0041]b. Insert the treated stem segment into the starting induction solid medium DKW+TDZ 0.5mg / L+IBA 0.05mg / L, the temperature is 23±1°C, the light is 2500lx, the time is 16h / d, and the induction culture is carried out for 22 days;

[0042] c. Cut the induced new long axillary buds into stems with one axillary bud and insert them into solid medium DKW+6-BA 0.5mg / L+IBA 0.001mg / L, place them at a temperature of 23±1°C, and light at 3000°C lx, time 16h / d for cultivation;

[0043] d. Cut off the 1.2cm seedlings, put their roots into the routinely steri...

Embodiment 3

[0047] a. Cut Xinwen No. 185 field walnut branches, wash them with tap water for 1.5 hours, then cut the branches into 2cm stem sections, each stem section contains an axillary bud, and then put the stem sections on the ultra-clean workbench with 75% Alcohol surface disinfection for 70 seconds, 0.1% mercury liter sterilization for 12 minutes, 15% hydrogen peroxide treatment for 30 minutes, and sterile water rinse for 5 times;

[0048] b. Insert the treated stem segment into the starting induction solid medium DKW+TDZ 1.2mg / L+IBA 0.15mg / L, the temperature is 23±1°C, the light is 2700lx, the time is 16h / d, and the induction culture is carried out for 25 days;

[0049] c. Cut the induced new long axillary buds into stems with one axillary bud and insert them into solid medium DKW+6-BA 1.2mg / L+IBA 0.008mg / L, place them at a temperature of 23±1°C, and light at 3200°C lx, time 16h / d for cultivation;

[0050] d. Cut out the 1.5cm-grown seedlings, insert them into solid medium 1 / 4DKW...

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Abstract

The invention relates to a method for tissue culture and rapid propagation of a thin-skinned walnut in Xinjiang; the method comprises the following steps of the obtaining of an aseptic seedling; multiplication of sprout; rooting of the sprout; training of seedlings and transplantation; a sterilization treatment method for field walnut branches, optimum culture medium for the multiplication of sprout and culture medium for the rooting of the sprout are provided; the invention has the advantages of simple method, rapid propagation speed, good stability, short culturing period, high rooting ratio, good quality of root, easy survival for transplantation and stronger feasibility in the practical production and application, is applicable to industrial culture of seeding of the thin-skinned walnut in xinjiang and is beneficial to solve the practical problem that the fine variety seedlings are lacking of supplies for a long time.

Description

technical field [0001] The invention relates to the field of plant tissue culture and rapid propagation, in particular to a method for tissue culture and rapid propagation of Xinjiang thin-skinned walnuts. Background technique [0002] Walnut (Juglans regia L.), known as walnut or Qiang peach in ancient times, belongs to Juglandaceae (Juglandaceae) and belongs to the genus Juglans. It is native to southeastern Europe and western Asia. my country is also one of the origins of walnuts. Because walnut is an excellent economic tree species with both fruit and material, and as one of the world's four major dried fruits (walnut, almond, cashew nut, hazelnut), it is widely cultivated all over the world with its high economic, ecological and social benefits. my country has cultivated walnuts for more than 2,000 years. According to legend, Zhang Qian of the Western Han Dynasty traveled to the Western Regions more than 2,000 years ago and introduced walnuts to my country from Persia (...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00C12N5/04A01G31/00
Inventor 王晓军胡石开郝秀英刘敏徐琴刘传军
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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