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41results about How to "Good for transplanting" patented technology

Method for inducing blueberries to root by using liquid culturing media and permeable matrix

The invention discloses a method for inducing blueberries to root by using liquid culture media and permeable matrix, comprising the following steps: (1) preparing rooting culturing media; (2) transferring the healthy and strong blueberry seedlings which are subcultured for 40d and are 3-4 cm high into the rooting culturing media, culturing the blueberry seedlings for 7-10d in the dark and for 12h/d for about 20d under intermitted illumination condition until the blueberry seedlings have 3-7 root systems of 0.5-2 cm long; and (3) transplanting the test-tube blueberry seedlings which are cultured for 30d to root, are 3-6 cm high and have the root length of 0.5-2 cm into the media in which the proportion of turfy soil to moss is 1:1, and managing the blueberry seedlings. The rooting culturing media is prepared according to the following substeps: 1) preparing 0.8 cm*1.2 cm filtering paper balls or defatted cotton balls or 0.2 cm*0.6 cm vermiculites which function as the matrix of the rooting culturing media; 2) preparing rooting culturing solution which has the WPM of 1/2, the IBA of 1.0-5.0 mg.L-1, the edible white sugar of 2 percent and the pH value of 5.0-5.2; and 3) adding 6-10 filtering paper balls or defatted cotton balls or 20-30 vermiculites which are soaked by the rooting culturing solution and adding 3-5 ml rooting culturing solution to each culturing bottle and capping the culturing to sterilize according to the routine method. The invention has short rooting period and low production cost, can ensure that 100 percent of the blueberry seedlings can root and over 95 percent of the transplanted blueberry seedlings can survive and realize the rapid industrialized propagation of the blueberry seedlings on a large scale.
Owner:LUDONG UNIVERSITY

Method for improving tissue culture reproductive speed of Alpinia zerumbet

The invention relates to a method for improving tissue culture reproductive speed of Alpinia zerumbet, which relates to the tissue culture technology of Alpinia zerumbet, and comprises the following steps that: A. seed collection is proceeded after 7 to 8 continuous sunny days, clumped Alpinia zerumbet plants which are used as seed are dug out from the soil 2 to 3 days before the seed production, soil is removed, and the Alpinia zerumbet plants are hung upside down to be dried for 2 to 3 days at a cool well-ventilated indoor place; B. lateral buds on tubers or spouts are selected as explant materials; C. adventitious buds are inducted to produce; D. adventitious buds are bred and cultured; E. adventitious buds are rooted to culture; and F. seedlings are transplanted. The rapid formation of adventitious buds is promoted through high temperature and high-concentration hormone, so the induction efficiency is improved, and the culture time is shortened; the formation of variegated leaf solid buds can be realized by changing the culture medium formula, reducing the culture temperature and increasing the light density, whitening variation can be reduced, the rapid breeding rate and seedling formation rate can be effectively improved, the factory-oriented production progress of the tissue cultured seedling can be accelerated, and the production cost can be reduced.
Owner:东莞市生物技术研究所

Agricultural transplant device

The invention relates to the field of agricultural equipment, in particular to an agricultural transplant device. The agricultural transplant device comprises a support frame, an adjustment mechanism,a seedling removing mechanism and a transplanting mechanism; the adjustment mechanism is mounted on one side of the support frame, the transplanting mechanism is fixedly connected with the adjustmentmechanism, the seedling removing mechanism is fixedly connected with the support frame and located above the transplanting mechanism, and the transplanting mechanism comprises a lifting component, adriving component, a transmission component and a transplanting component, the lifting component is fixedly connected with the adjusting mechanism, the transplanting component is fixedly connected with the lifting component, the driving component is mounted on one side of the transplanting component, and the transplanting component and the driving component are in transmission connection through the transmission component. The agricultural transplant device has the advantages that the device does not need to be driven by pedals, and can automatically return to facilitate seedling feeding, so that the labor intensity and the amount of labor of farmers can be further reduced, and the seedling transplanting efficiency can be improved.
Owner:QINGDAO AGRI UNIV

Hormone-free corn callus regeneration method

The invention discloses a hormone-free corn callus regeneration method. The hormone-free corn callus regeneration method comprises the following steps: culturing granular corn calluses with good growth vigor on a hormone-free embryoid induction culture medium at 24-26 DEG C for 20-23 days, and inducing to generate an embryoid; transferring the embryoid to a hormone-free regeneration culture medium, wherein the culturing temperature is 24-26 DEG C, the illumination intensity is 12000lux-18000lux and the illumination time each day is 16h; culturing until roots and buds are generated to obtain a regenerated plant; and then continually culturing the regenerated plant for 6-7 days to raise strong seedlings under the following conditions that the temperature is 26-28 DEG C, the illumination intensity is 24000lux-30000lux and the illumination time each day is 16h. According to the hormone-free corn callus regeneration method, the osmotic pressure of a regeneration culture phase is regulated and controlled by changing the use amount of sucrose so as to realize the efficient regeneration of the calluses, and the whole process does not need plant hormones. The method not only saves the experiment cost, but also avoids poisoning tissue materials due to application of hormones for a long time.
Owner:CROP RES INST GUANGDONG ACAD OF AGRI SCI

Root induction method for Chinese fir test-tube plantlets

InactiveCN105660416ASolve the problem of rootingIncreased survival rate of hardened seedlings transplantedHorticulture methodsPlant tissue cultureHigh concentrationGreenhouse
The invention provides a root induction method for Chinese fir test-tube plantlets. The method specifically includes the steps that the robust Chinese fir test-tube plantlets are inoculated to high-concentration auxin to be stimulated and induced to root and then are inoculated to an NAA auxin solution, the test-tube plantlets obtained after stimulation treatment are inoculated into a blank culture medium to be cultured for 1 day, the Chinese fir test-tube plantlets are treated in the NAA auxin solution and then are cultured at the constant temperature of 25 DEG C under LED red light, and root induction culture of the Chinese fir test-tube plantlets can be completed; the Chinese fir test-tube plantlets obtained after root induction culture are transplanted into a greenhouse and are hardened at the room temperature; the base parts of the Chinese fir tissue-cultured plantlets are treated with ABT1+IAA+yellow mud after the culture medium is cleaned away, then the hardened Chinese fir plantlets are transplanted to a light-medium culture cup, and rooting treatment of the Chinese fir test-tube plantlets is completed. According to the method, after stimulation treatment is carried out with high-concentration auxin ANN, root source bases can be easily formed; then, the Chinese fir test-tube plantlets are transferred into the blank culture medium, so that elongation growth of the root source bases is promoted; the rooting percentage is increased to 95.8% from 10%.
Owner:BAODING UNIV

Rolling type small flowerpot soil spreading device used for garden for simulating of running robot

InactiveCN113079874AAvoid affecting transplanting workRealize the rolling functionSievingScreeningAgricultural engineeringEnvironmental geology
The invention discloses a rolling type small flowerpot soil spreading device used for a garden for simulating of a running robot. The rolling type small flowerpot soil spreading device comprises a box body, wherein the bottom parts of two sides of the box body are independently provided with supporting frames; the bottom of each supporting frame is provided with a supporting foot; the top of the box body is provided with a material feeding hopper; the bottom of the box body is provided with a material outlet; a material discharging part is arranged below the material outlet; a rolling device is arranged in the box body; the lower part between the supporting frames on two ends is provided with a fixing frame; in addition, two sides of the fixing frame are independently fixedly connected with the lower parts of the corresponding side edges of the supporting frames through a horizontal plate; and a flowerpot body is arranged in the fixing frame. The rolling type small flowerpot soil spreading device has the beneficial effects that the rolling device capable of simulating the structural form of the running robot is adopted to roll soil to avoid a phenomenon that caking soil directly falls into the flowerpot to affect subsequent transplantation work, meanwhile, working labor force of a manual operation is reduced, and working efficiency is improved.
Owner:夏爱莲

Plant tissue culture method for promoting strong roots of potato seedlings

The invention relates to the field of plant tissue culture, in particular to a tissue culture method for promoting strong roots of potato seedlings. The tissue culture method comprises the following steps: (1) carrying out material collection and disinfection; (2) stripping stem tips: stripping outer layer leaves of the sterilized potato apical buds under a binocular dissection mirror, and leavingonly 1-2 leaf primordia; (3) carrying out primary culture: inoculating 5-6 stem tips with sizes of 0.1-0.2mm in a primary culture medium for culturing; (4) carrying out rooting culture: inoculating the sterile seedlings, obtained in step (3), in a rooting culture medium in a form of single plants for culturing so as to obtain virus-free test-plantlets. The tissue culture method provided by the invention utilizes stem tip in vitro culture to solve the problem of large consumption of seed potatoes; furthermore, the primary culture medium, the rooting culture medium and culture conditions are optimized, so that the method not only can enable potatoes to have more roots and rapidly take roots, but also can enable the tissue cultured seedlings of the potatoes to be healthy and strong so as toenable the seedlings to be more easily transplanted and more easily survive.
Owner:WUHAN YEDONGLI BIOTECH CO LTD

Cunninghamia lanceolata tissue culture seedling transplanting matrix and preparation method thereof

The invention relates to the technical field of plant cultivation, in particular to a cunninghamia lanceolata tissue culture seedling transplanting matrix and a preparation method thereof. The transplanting matrix comprises the following components in parts by volume: 1-3 parts of fermented bark, 2-3 parts of fermented wood bran, 4-5 parts of foamed coconut bran and 0.5-1 part of yellow soil. According to the invention, by taking the bark, the wood bran and the coconut bran as main materials, and the yellow soil as an auxiliary material, the cunninghamia lanceolata tissue culture seedling transplanting matrix is prepared, so that the content of the organic matters in the bark is high, the antibacterial property and the stress resistance are achieved, and the content of organic matters in the bark is high, including cellulose, hemicellulose, lignin, or other microelement; the carbon-nitrogen ratio of the wood bran is high, and the air permeability and the water-retaining property are good, the coconut bran is loose in texture, natural decomposition is slow, and the service life of the matrix can be prolonged, the texture of the yellow soil is compact, the loss of moisture and nutrients can be effectively prevented, and the prepared matrix is very suitable for transplanting cunninghamia lanceolata tissue culture seedlings.
Owner:GUANGXI FORESTRY RES INST

Method for plug-tray greenwood cutting seedling culture of rhododendron ovatum

PendingCN111316898AIncrease rooting rate of cuttingsSave seedling substrateCultivating equipmentsSoilless cultivationAxillary budRhododendron ovatum
The invention discloses a method for plug-tray greenwood cutting seedling culture of rhododendron ovatum. A semi-lignified twig grown in a current year is clipped and taken as a cutting, and a cuttingshoot is clipped from the cutting and soaked in an ABT reagent of concentration of 50-100 mg/L for 4-6 h; after soaking, the cutting shoot is inserted into a seedling culture plug tray for cutting, thorough watering is conducted immediately after cutting, a sterilizing agent is sprayed, and the seedling culture plug tray is coated with a thin film and shielded; and then, watering is conducted incase of dryness, and transplantation can be conducted when an axillary bud of the cutting shoot germinates and a new root of the cutting shoot grows to 1-3 cm. In this way, the method can greatly increase a cutting rooting rate of rhododendron ovatum, can increase a cutting survival rate of rhododendron ovatum to about 73.33%, can save a matrix and space of seedling culture, does not need establishment of a dedicated arc shed, realizes good moisture preservation effects, does not need frequent water supplementation, is simple and easy in operations, low in production cost and high in economicbenefits, can promote transplantation survival and has a high application value.
Owner:SUZHOU GARDEN DEV +1

A working method of an agricultural transplanter

The invention relates to the field of agricultural equipment, specifically to an agricultural transplanter. The agricultural transplanter comprises a supporting frame, an adjusting mechanism, a seedling feeding mechanism and a transplanting mechanism, wherein the adjusting mechanism is arranged at one side of the supporting frame; the transplanting mechanism is fixedly connected with the adjustingmechanism; the seedling feeding mechanism is fixedly connected with the supporting frame and located above the transplanting mechanism which comprises a lift-drop assembly, a driving assembly, a transmission assembly and a transplanting assembly; the lift-drop assembly is fixedly connected with the adjusting mechanism; the transplanting assembly is fixedly connected with the lift-drop assembly; the driving assembly is arranged at one side of the transplanting assembly; and the transplanting assembly and the driving assembly are in transmission connection through the transmission assembly. Theagricultural transplanter provided by the invention has the following beneficial effects: the agricultural transplanter does not need a pedal to drive, can automatically return, facilitates feeding seedlings, can further reduce the labor intensity and labor amount of a farmer and improves the efficiency of seedling transplanting.
Owner:QINGDAO AGRI UNIV

Method for improving tissue culture reproductive speed of Alpinia zerumbet

The invention relates to a method for improving tissue culture reproductive speed of Alpinia zerumbet, which relates to the tissue culture technology of Alpinia zerumbet, and comprises the following steps that: A. seed collection is proceeded after 7 to 8 continuous sunny days, clumped Alpinia zerumbet plants which are used as seed are dug out from the soil 2 to 3 days before the seed production, soil is removed, and the Alpinia zerumbet plants are hung upside down to be dried for 2 to 3 days at a cool well-ventilated indoor place; B. lateral buds on tubers or spouts are selected as explant materials; C. adventitious buds are inducted to produce; D. adventitious buds are bred and cultured; E. adventitious buds are rooted to culture; and F. seedlings are transplanted. The rapid formation of adventitious buds is promoted through high temperature and high-concentration hormone, so the induction efficiency is improved, and the culture time is shortened; the formation of variegated leaf solid buds can be realized by changing the culture medium formula, reducing the culture temperature and increasing the light density, whitening variation can be reduced, the rapid breeding rate and seedling formation rate can be effectively improved, the factory-oriented production progress of the tissue cultured seedling can be accelerated, and the production cost can be reduced.
Owner:东莞市生物技术研究所

A kind of method of banana MS-free tissue culture

The invention provides a method for tissue culture of bananas without MS. In the banana cluster bud proliferation stage, the subculture stage and the rooting culture stage, the cluster bud proliferation tissue culture fluid, the subculture tissue culture fluid and the root tissue culture fluid are respectively used. The cassava ethanol anaerobic fermentation broth medium is included in the cluster bud proliferation tissue culture fluid, the subculture tissue culture fluid and the rooting tissue culture fluid. Adopting the technical scheme of the present invention, using the cassava alcohol anaerobic fermentation liquid to replace the basic medium MS, not only does not have any influence on the normal growth of the three different stages of banana tissue culture, but also greatly reduces the production cost, turning waste into wealth, and improving ecological quality. The environment is more environmentally friendly, and the cultivated banana seedlings can also achieve the effect of the seedlings cultivated by MS tissue culture in the past, and the number and sturdiness of the roots are more obvious. The greening period is earlier than the basic MS medium, and it is easier to transplant the tissue culture seedlings Survival provides favorable conditions for large-scale tissue culture production.
Owner:GUANGXI UNIV +1

A plant tissue culture method for promoting strong roots of potato seedlings

The invention relates to the field of plant tissue culture, in particular to a tissue culture method for promoting strong roots of potato seedlings. The tissue culture method comprises the following steps: (1) carrying out material collection and disinfection; (2) stripping stem tips: stripping outer layer leaves of the sterilized potato apical buds under a binocular dissection mirror, and leavingonly 1-2 leaf primordia; (3) carrying out primary culture: inoculating 5-6 stem tips with sizes of 0.1-0.2mm in a primary culture medium for culturing; (4) carrying out rooting culture: inoculating the sterile seedlings, obtained in step (3), in a rooting culture medium in a form of single plants for culturing so as to obtain virus-free test-plantlets. The tissue culture method provided by the invention utilizes stem tip in vitro culture to solve the problem of large consumption of seed potatoes; furthermore, the primary culture medium, the rooting culture medium and culture conditions are optimized, so that the method not only can enable potatoes to have more roots and rapidly take roots, but also can enable the tissue cultured seedlings of the potatoes to be healthy and strong so as toenable the seedlings to be more easily transplanted and more easily survive.
Owner:WUHAN YEDONGLI BIOTECH CO LTD

Xinjiang Hotan rose rhizome seedling raising and large-scale cultivation method

InactiveCN111631070AGood genetic stabilityLow requirements for planting conditionsCultivating equipmentsVegetative propogationRhizomeTransplanting
The invention relates to a Xinjiang Hotan rose rhizome seedling raising and large-scale cultivation method. The method comprises the steps of seedling raising material selection, nutrient soil adaptation, root burying seedling raising, transplanting and field management. By means of the method, standard Hotan rose planting operation regulations are established, and rapid artificial seedling raising, field cultivation and standard planting are provided, and moreover, the propagation period of the Hotan roses is shortened, the method is suitable for industrial cultivation of the Hotan roses, anda foundation is laid for rapid propagation of the Hotan roses. Technical support is provided for forming a Hotan rose industry chain, so that the special species generate huge economic benefits; anda scientific basis is provided for excellent variety breeding and rapid propagation research of the Hotan roses. The method solves the key problems of quality real-time evaluation standard, high addedvalue product development, comprehensive resource utilization and the like suitable for Hotan rose variety breeding, large-scale artificial planting, seedling culture and planting processes in the southern Xinjiang region.
Owner:XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI

Method for inducing rooting of Chinese fir test-tube seedlings

The invention provides a root induction method for Chinese fir test-tube plantlets. The method specifically includes the steps that the robust Chinese fir test-tube plantlets are inoculated to high-concentration auxin to be stimulated and induced to root and then are inoculated to an NAA auxin solution, the test-tube plantlets obtained after stimulation treatment are inoculated into a blank culture medium to be cultured for 1 day, the Chinese fir test-tube plantlets are treated in the NAA auxin solution and then are cultured at the constant temperature of 25 DEG C under LED red light, and root induction culture of the Chinese fir test-tube plantlets can be completed; the Chinese fir test-tube plantlets obtained after root induction culture are transplanted into a greenhouse and are hardened at the room temperature; the base parts of the Chinese fir tissue-cultured plantlets are treated with ABT1+IAA+yellow mud after the culture medium is cleaned away, then the hardened Chinese fir plantlets are transplanted to a light-medium culture cup, and rooting treatment of the Chinese fir test-tube plantlets is completed. According to the method, after stimulation treatment is carried out with high-concentration auxin ANN, root source bases can be easily formed; then, the Chinese fir test-tube plantlets are transferred into the blank culture medium, so that elongation growth of the root source bases is promoted; the rooting percentage is increased to 95.8% from 10%.
Owner:BAODING UNIV
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