69results about How to "Promote late growth" patented technology

The invention relates to a tobacco package fertilization method, which is mainly characterized in that three kinds of fertilizers: special tobacco active biological organic and inorganic fertilizers, special tobacco seedling extracting fertilizers and special tobacco additional fertilizers are adopted, and the fertilizers with different nutrition mixture ratios are adopted according to different growth periods of the tobacco for promoting the growth of the tobacco. The tobacco package fertilization method is guided by a tobacco fertilization demanding rule, different forms of fertilizers withdifferent nutrients are applied in different field growth periods of the tobacco for promoting the tobacco to realize the better growth, and the fertilization method has the effects of simplifying the tobacco farmer work procedure, reasonably balancing the tobacco fertilization, improving the tobacco use value and the like, so the goals of reducing the work intensity of tobacco farmers, enhancingthe efficiency, improving the quality of the tobacco leaves and increasing the yield are reached.

The invention provides a process for producing the oriental lily sterilized seed globule in the bottle, comprising: choosing explant, sterilizing, inducing culture and getting the sterilized stem tip, then breeding culture, subculture, and producing the sterilized seed globule in the bottle directly. The invention produces the sterilized seed globule in the bottle directly to avoid the problem of long period of ball forming and the accumulation of virus existing in the oriental lily, the seed globule can be planted in the soil when out of the bottle, and the survival rate is high. The breeding procedure of the sterilized seed globule is simplified by modifying and optimizing the whole producing procedure for the lily seed globule, and the breeding time of sterilizing the oriental lily is shortened greatly, the quality of seed globule is improved when the producing cost is decreased, which reinforces market competitive power of the domestic self-breeding seed globule and is suitable for the mass production of the oriental lily sterilized seed globule.

The invention provides a method for separating and culturing mouse cells, comprising the following steps: (1) disinfecting tissue processing instruments; (2) selecting male BALB/c mice aged 1 to 2 months, killing them by dislocation of the neck, and using surgical scissors Separate the synovial layer and fibrous layer of the joint capsule, then take out the synovial layer tissue, put the tissue into pre-cooled sterile double-antibody-containing PBS solution and wash several times to remove blood stains and fat tissue; (3) Cut the tissue into pieces and use Digest with preheated type II collagenase and trypsin respectively; (4) terminate the digestion, centrifuge, and discard the supernatant; (5) inoculate into the coated culture flask by artificial adherence method; (6) after incubation for 1-2 hours, Cultured in an environment of 37°C and 5% CO2; (7) observation of cell morphology; (8) subculture of cells; (9) identification of cells. The method for isolating and culturing mouse synoviocytes provided by the invention is simple to operate, has a large number of cells and a high survival rate, and is an ideal primary method for isolating and culturing mouse synoviocytes, providing a reliable basis for experiments. cellular resources.

The invention discloses an organic rice cultivation method by utilizing farmland and rice chaff weeding, belonging to the technical field of rice production. The method comprises the steps of: soaking in shallow water; ploughing for two times or three times; transplanting strong seedlings; broadcasting rice chaff; conducting later field management and other steps. In the method, drainage is required at first and then ploughing is carried out each time, which aims at carrying away sheathed monochoria herb without spouting and floating on the surface of a water level and seeds of chufa through drainage; the rice chaff fertilization faster improve the field temperature so as to create a good sprouting environment for weed seeds, and also can reduce the oxygen content under the surface layer, thus preventing the normal growth of the weed roots and achieving purpose of killing weeds, further increasing the content of organic matters, being beneficial to improvement of rice quality. The organic rice cultivation method can be popularized and applied in rice cultivation regions.

The invention provides a separating and culturing method for olfactory ensheathing cells of rats. The separating and culturing method comprises the following steps: (1) disinfecting a tissue processing instrument; (2) selecting male SD rats of 2-3 months old, killing the male SD rats after anesthesia, separating the olfactory mucosa tissue by adopting surgical scissors, placing the tissue in PBSsolution which is precooled, is sterile and contains double antibodies, and washing the tissue for a plurality of times, so that blood stains and outer membranes are removed; (3) cutting up the tissue, and carrying out digestion by adopting preheated neutral enzyme and trypsin respectively; (4) terminating the digestion, carrying out centrifugation, and discarding the supernatant; (5) inoculatingthe obtained product into an enveloped culture flask by adopting an artificial wall sticking method; (6) carrying out incubation for 1-2 h, and carrying out culturing in the environment being at 37 DEG C containing 5% CO2; (7) carrying out cell purification; (8) carrying out cell morphological observation; (9) carrying out cell subculturing; (10) carrying out cell counting and survival rate testing; and (11) carrying out immunological identification. For the separating and culturing method for olfactory ensheathing cells of rats, the operation is simple, a large number of cells are obtained, the survival rate is high, the method belong to an ideal primary separating and culturing method for the olfactory ensheathing cells of rats, and a reliable cell resource is provided for the experiment.

The invention relates to the technical field of restoration of the ecological environment in mining areas, in particular to a planting method for water and fertilizer conservation on mine slopes, planting tree bags and solid pits, which is suitable for slopes with small slopes. Drill the nail pile under the tether, take soil samples and test, determine the amount of water-retaining agent and rooting powder according to the test results, fully mix the water-retaining agent with the soil, organize digging, organize pipeline laying, and tree planting, use 0.4~0.8×0.3~0.8 m Planting bags for water and fertilizer conservation, setting up soil retaining frames, daily maintenance, focusing on organizing watering, fertilization and weeding according to the growth habits of seedlings, and strengthening the prevention and control of diseases and insect pests. The invention can promote the development and utilization of mineral resources, stepping into a virtuous circle with good economic benefits, low resource consumption and less environmental pollution. It provides a set of fast, economical and practical methods for the ecological restoration of mining areas in the future and the ecological restoration of mining areas in similar areas at home and abroad.

The invention relates to a formula of base fertilizer and leaf fertilizer for increasing apple production. According to the formula, thoroughly decomposed waste bacteria sticks, thoroughly decomposed dung, thoroughly decomposed Chinese medicine residues, fine sand and microelement fertilizer are mixed according to a certain metering standard to obtain the base fertilizer, and biogas slurry and amino acid fertilizer are diluted with water to obtain the leaf fertilizer. According to the formula of the base fertilizer and leaf fertilizer for increasing the apple production, the base fertilizer is applied according to a standard of 250-300kg each tree in autumn, and the leaf fertilizer is applied to the crown of each apple tree in April of the next year; after the application of the base fertilizer and the leaf fertilizer, the waste bacteria sticks, nutrient substances such as organic matter contained in the dung and the Chinese medicine residues can promote the growing of roots and healing of broken roots, microelement can promote the later-period growing of apple trees, and meanwhile, various components are matched with one another to improve the soil property and lay a firm foundation for the high yield of the apple trees in the next year.

The invention discloses a furrowing profiling pneumatic type precision vegetable seeder. The precision vegetable seeder comprises a main frame and a profiling unit. A three-point suspension rack is fixed at the front end of the main frame. A seeding unit is fixed at the rear end of the main frame. A pneumatic unit is fixed above the main frame. A furrowing shaping unit is fixed below the main frame. The profiling unit is mounted on the main frame. The furrowing profiling pneumatic type precision vegetable seeder has the advantages that operations such as furrowing, compacting and seeding can be completed in one step, and the seeder is high in operation efficiency and high in adaptability.

The invention discloses a biological concentrate supplement for breeding fat cattle and sheep. The biological concentrate supplement for breeding fat cattle and sheep is prepared from the following active ingredients in parts by weight: 10-15 parts of astragalus membranaceus, 10-15 parts of herba epimedii, 4-8 parts of semen cuscutae, 4-8 parts of sweet wormwood, 4-8 parts of folium isatidis, 10-15 parts of hawthorn fruits, 1-3 parts of procyanidin and 1-3 parts of lactobacillus sporogenes. The biological concentrate supplement for breeding fat cattle and sheep is capable of resisting bacteria and diminishing inflammation, tonifying spleen and promoting appetite, enhancing the disease resisting ability and the anti-stress ability and enhancing the immunity of cattle and sheep, contains no antibiotics, and is capable of increasing the feed conversion ratio, reducing the breeding cost, shortening the production period, increasing the dressing percentage, improving the flavor of meat and improving the breeding efficiency.

The invention discloses an adjustable white celery soil hilling device which comprises two spiral soil borrowing and feeding mechanisms, two soil hilling molds, two fixing racks, soil hilling height adjusting mechanisms, a soil hilling width adjusting mechanism, two compacting and seedling gathering mechanisms and two soil hilling foundation compacting plates. The spiral soil borrowing and feeding mechanisms are fixed to the front ends of the two fixing racks, the two soil hilling molds are arranged on the inner sides of the spiral soil borrowing and feeding mechanisms, the soil hilling height adjusting mechanisms are symmetrically fixed to the two fixing racks, and the soil hilling width adjusting mechanism comprises two adjusting seats and two adjusting cross beams. The two compacting and seedling gathering mechanisms are symmetrically fixed to the front sides of the soil hilling molds, and the two soil hilling foundation compacting plates are fixed below the outer sides of the two soil hilling molds. The adjustable white celery soil hilling device can simultaneously adjust the width and height of white celery soil hilling and is simple in structure and convenient to operate. Soil block compacting plates can compact big soil blocks on two sides of seedlings, compact soil and ensure firm soil hilling, and seedling gathering frames can gather and centralize white celery seedlings lodging on two sides of the seedlings.

The invention provides a method for raising tea seedlings by cutting in winner and relates to the technical field of tea seedling raising. The method is characterized by comprising the following steps of twig picking, wherein semi-lignification twigs reserved in a female parent garden are picked; twig soaking, wherein the picked twigs are placed in a soak solution for soaking, the soak solution is prepared from, by weight, 4-6 parts of sepiolite powder, 20-50 parts of purified water, 1-2 parts of nonylphenol polyoxyethylene ether, 3-4 parts of tartaric acid and 1-3 parts of potassium oxalate; twig airing, wherein the twigs obtained after soaking are aired in the sun, and the content of moisture in the twigs obtained after airing ranges from 30 to 40; cuttage and scissoring, wherein a cuttage method comprises the steps that the cuttage plant spacing is 3 centimeters, the line spacing is 8 centimeters, the number of cuttage plants per mu ranges from 220,000 to 250,000, two thirds of each plant is inserted into soil, buds and petiole are exposed out of the soil surface, soil around cuttings is compacted, and watering and cuttage are conducted simultaneously. By means of the method, moisture is controlled reasonably, the survival rate of the cuttings is high, and later-period growth is promoted.

The invention provides a human primary hepatocyte separation and culture method. The method comprises the following steps of (1) cutting a fresh human hepatocyte under an aseptic condition, sealing at4 DEG C and then carrying back to a lab; (2) placing the tissue into a precooled sterile PBS buffer liquid containing double antibodies, and washing for multiple times to remove blood and connectivetissues; (3) irrigating through a preheated PBS buffer liquid through a needle-punching method; (4) irrigating through a preheated HBSS buffer liquid through a needle-punching method; (5) irrigating through a preheated GBSS mixed enzyme liquid through a needle-punching method; (6) cutting up the tissue, and using the GBSS mixed enzyme liquid for digesting, centrifuging, and removing a supernatant;(7) using trypan-blue for dyeing a cell filter liquor to detect cell activity; (8) after carrying out cell counting, resuspending a complete medium, inoculating into a coated culture flask, and culturing at 37 DEG C in an environment with 5 percent of CO2; (9) identifying cell morphology; (10) detecting through an ELISA (Enzyme-linked Immuno Sorbent Assay) method; (11) detecting through RT-PCR (Reverse Transcription-Polymerase Chain Reaction). The invention provides the human primary hepatocyte separation and culture method which is simple to operate, the number of the obtained cells is large, the survival rate is high, the method is the ideal human primary hepatocyte separation and culture method, and a reliable cell resource is provided for experiments.

The invention discloses a method for improving the rhus succedanea seed germination rate and accelerating germination. The method comprises the following steps that (1) ripe and full rhus succedanea seeds are selected to get peels removed and disinfected, and then the treated seeds are dipped with 98% concentrated sulfuric acid for 20-25 minutes so as to accelerate germination; and (2) the seeds obtained in the step (1) are dipped with a gibberellin solution with the concentration being 300mg/L for 12 hours, the seeds are washed cleanly under flowing water after being treated, and the cultureconditions are controlled to conduct germination acceleration. The method can omit the steps of long-time seed soaking and stratification treatment, rapidly breaks seed dormancy of rhus succedanea, effectively improves the seed germination rate, and the germination rate is as high as 98% or above. Meanwhile, the germination time is greatly shortened, the seeds can germinate completely after about7-10 days, the purpose of rapid gemination acceleration is achieved, germination is tidy and consistent, and operation management such as seedling transplanting and field planting in the following period is facilitated.