Method for artificially inoculating pine wood nematodes by semiannual masson pine seedling tender tip skin scratch and application thereof

A technology of pine wood nematode and artificial inoculation, which is applied in the direction of animal husbandry and the like, can solve the problem of no masson pine seedlings inoculated with pine wood nematode, etc., and achieve the effects of improving inoculation efficiency, facilitating inoculation, and saving cultivation costs.

Active Publication Date: 2019-02-15
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore up to the present, do not see the method report of inoculating the method report of pine wood nematode inoculated with the successful half-year-old masson pine seedling that is urgently needed in production

Method used

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  • Method for artificially inoculating pine wood nematodes by semiannual masson pine seedling tender tip skin scratch and application thereof
  • Method for artificially inoculating pine wood nematodes by semiannual masson pine seedling tender tip skin scratch and application thereof
  • Method for artificially inoculating pine wood nematodes by semiannual masson pine seedling tender tip skin scratch and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Inoculation of young shoot epidermis inoculation with pine wood nematodes

[0042] (1) adopt conventional method, a large number of live pine wood nematodes are cultivated;

[0043] (2) prepare the suspension of live pine wood nematode, concentration is 3000 / 200 microliters;

[0044] (3) Select 16 half-year-old masson pine seedlings with better growth, among which 10 strains are inoculated with 3000 pine wood nematodes, and 6 strains are used as blank controls to inoculate double distilled water. For the young shoots at about 3-5cm, pull down the needles 1.5-2cm;

[0045] (4) Use a sharp scalpel to draw two longitudinal wounds about 1.5 cm in length on the loose and tender epidermis where the needles have been removed, with a depth of 0.5 to 1 mm;

[0046] (5) Use a sealing film to form a small funnel around the part where the needles are removed. The diameter of the upper opening of the funnel is about 1.5cm to ensure that there will be no water leakage. Note...

Embodiment 2

[0054] Pine xylophilus inoculation of different concentrations of embodiment 2 semi-annual Pinus massoniana

[0055] According to the method of Example 1, different concentrations of B. xylophilus were inoculated. The difference from Example 1 was that the number of B. xylophilus inoculated was 200, 500, 1000, 2000, 3000, 4000, and 5000, totaling 7 treatments. Each treatment was repeated 10 times, and 6 Pinus massoniana seedlings inoculated with sterile water were used as controls. Observe the symptoms after inoculation, and count the total number of diseased strains 7, 10, 13, 16, 18, and 21 days after inoculation.

[0056] The result is as figure 2 As shown, the research results show that 7 days after inoculation, pine seedlings inoculated with 2000, 3000, 4000 and 5000 pine wood nematodes began to develop disease; The plants began to fall ill; the plants inoculated with 200 pine xylophilus plants did not suffer from the disease. As the number of days of inoculation incr...

Embodiment 3

[0057] Example 3 Observation on the Symptom Development Process of Pine Xylophilus Disease of Highly Susceptible Pinus massoniana

[0058] Once the half-year-old Pinus massoniana develops disease, it may die later due to its low resistance. Therefore, it can be designated as a high-resistant Pinus massoniana plant (no disease) or a resistant Pinus massoniana plant (slow disease onset) according to the time of onset and death. ) and high-sensitivity Masson pine plants (fast onset); specifically: high-sensitivity Masson pine does not develop disease; resistant plants generally inoculate for more than 10 days, or even longer to start to develop symptoms, and will die after 6-10 days or even longer after symptoms appear ; High-susceptibility plants generally begin to develop symptoms 8 to 10 days after inoculation, and most of them die after 3 to 5 days of symptoms.

[0059] According to the method of embodiment 1, adopt young shoot epidermis scratching inoculation method to 67 ha...

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Abstract

The invention discloses a method for artificially inoculating pine wood nematodes by a semiannual masson pine seedling tender tip skin scratch and an application thereof. The method comprises the following steps of: (1) preparing living pine wood nematodes into a pine wood nematode suspension; (2) selecting a tender tip position 3-5cm below the tip of a semiannual masson pine seedling, pulling outneedle leaves, and longitudinally scratching two or more wounds at the skin of the tender tip of the masson pine from which the needle leaves are pulled out; (3) enclosing a small water-tight funnelat the part where the needle leaves are removed by using a sealing film; wherein the lower part of the small funnel clings to the tender tip of the masson pine and is flush with the lower part of thewounds; (4) injecting the pine wood nematode suspension into the small funnel to inoculate the pine wood nematodes; and (5) placing the semiannual masson pine seedlings inoculated with the pine wood nematodes in an outdoor environment for cultivation. The method is suitable for artificially inoculating pine wood nematodes to the semiannual masson pine seedlings, can effectively reduce production cost, and has wide application prospect and important production significance.

Description

technical field [0001] The invention relates to a technique for artificially inoculating pine wood nematodes in masson pine seedlings, in particular to a method for artificially inoculating pine wood nematodes by scratching the tender shoots of half-year-old masson pine seedlings and an application thereof. Background technique [0002] Masson pine is an important timber tree species in China. It has a wide range of uses and is widely planted in 17 provinces in China. Among them, pine wood nematode disease occurred in many provinces, which brought great economic losses to forestry production. Planting disease-resistant strains of Masson pine is the fundamental measure to control the disease. Due to the large area of ​​pine xylophilus in China and the large differences in ecological conditions in different regions, according to the principle of suitable tree for the right place and the gene-to-gene hypothesis (Flor, 1971), local pine tree seedlings that are resistant to pine...

Claims

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Application Information

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IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 王新荣张佳雯李星星刘纯鑫姚剑平罗萌浩孙思程明赵利锋
Owner SOUTH CHINA AGRI UNIV
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