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Inoculation identification method for clubroot of cruciferous crops

A technology of cruciferous crops and identification methods, which is applied in the field of inoculation identification of clubroot of cruciferous crops, can solve the problems of large consumption of inoculated bacteria sources and insufficient stability of results, and achieve stable bacteria sources and reduce seedling The occurrence of damping-off or other rhizome diseases, and the effect of uniform incidence

Inactive Publication Date: 2017-08-18
INST OF PLANT PROTECTION JIANGXI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] For this reason, the object of the present invention is to have the problems of large consumption of inoculation bacteria source and insufficient stability of results in the inoculation of bacteria soil in the prior art, thereby giving clubroot pathogen physiological race identification and variety resistance identification and resistance genetic mechanism Studying Detrimental Defects Provides an Inoculation Identification Method for Clubroot in Cruciferous Crops

Method used

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  • Inoculation identification method for clubroot of cruciferous crops
  • Inoculation identification method for clubroot of cruciferous crops
  • Inoculation identification method for clubroot of cruciferous crops

Examples

Experimental program
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Effect test

Embodiment 1

[0036] In this example, a single root nodule sample from two different disease areas was used as the research object, and its race species attribute was identified by the Williams identification system. The identification method includes 4 identification hosts, all of which are Brassicaceae plants, namely Jersey Queen and Badger Shipper of Brassica oleracea var. capitata and Laurentian and Wilhelmsburger of B. napobrassica.

[0037] 1. Preparation of inoculum

[0038] The two root nodules were clubroot swollen tissues of Chinese cabbage and rapeseed collected from Nanchang and Wuyuan, Jiangxi. After adding a small amount of sterile water to homogenate, mix with sterile dry moss (no need to rinse), adjust the amount of dormant spores in the moss to 10 6 / g or more, for use.

[0039] 2. Inoculation method

[0040]Use 0.5% potassium permanganate to soak the seedling tray (hole depth 6cm, single hole diameter 6cm, single hole bottom diameter 3cm) to carry out surface disinfecti...

Embodiment 2

[0049] In this example, 11 varieties of rapeseed materials were taken as the research object, and their resistance to Plasmodium race 4 was identified by this method.

[0050] 1. Preparation of inoculum

[0051] Fresh root nodules were obtained from root nodules that had been identified as race 4. After adding a small amount of sterile water to homogenate, mix with sterile dry moss (no need to rinse), so that the amount of dormant spores in the moss reaches 10 6 / g or more, for use.

[0052] 2. Inoculation method

[0053] Use 0.5% potassium permanganate to soak the seedling cultivation tray (the depth of the hole is 6cm, the diameter of a single hole is 6cm, and the bottom diameter of a hole is 3cm) to carry out surface disinfection, and it is framed in a tray that is equipped with an aqueous solution (adding rapeseed formula fertilizer in water) at a depth of 10cm. The bottom of the seedling tray does not touch the aqueous solution in the tray. Insert an absorbent cotton t...

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Abstract

The invention discloses an inoculation identification method for clubroot of cruciferous crops, belongs to the technical field of plant protection, and provides the inoculation identification method for clubroot of cruciferous crops. Aseptic dried sphagnum is used as a carrier for culturing Plasmodiophora by inoculating. The method of the invention has the advantages that the consumption of bacterial sources is low, inoculating efficiency is high, operability is high, pathogenesis is uniform, result stability is good, disease determination is highly sensitive, the method is applicable to the identification of physiological races of small samples and the identification of disease resistance in large-batch varieties, and the method serves for breeding novel disease-resistant varieties and reasonably laying out disease-resistant varieties.

Description

[0001] Technical field: [0002] The invention relates to the technical field of plant protection, in particular to an inoculation identification method for clubroot of cruciferous crops. Background technique [0003] Cruciferous clubroot caused by Plasmodiophora brassicae is an important worldwide plant disease. The loss caused by the disease accounts for more than 10% of the total amount of cruciferous crops in the world, and the loss caused by the diseased area is generally 20% to 90%. The fungus mainly harms the roots of cruciferous plants, and can produce hormones such as indole acetic acid and cytokinin, causing the root tissues of plants to expand and form root tumors. my country is a country with a wide distribution of clubroot, and cruciferous crops are distributed in most provinces and cities. With the acceleration of urban-rural integration and the establishment of large-scale vegetable bases, clubroot is prevalent in a large area. Created conditions, but also brou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18C12Q1/04C12Q1/24A01G1/00
CPCA01G22/00C12Q1/04C12Q1/18C12Q1/24
Inventor 黄蓉黄瑞荣胡建坤
Owner INST OF PLANT PROTECTION JIANGXI ACAD OF AGRI SCI
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