Root induction method for Chinese fir test-tube plantlets

A technology for test-tube seedlings and Chinese fir, which is applied in the field of inducing rooting and culturing of Chinese fir plants, can solve problems such as difficult rooting of fir test-tube seedlings, and achieves the effects of overcoming the difficulty in inducing rooting of test-tube seedlings, improving the survival rate, and solving the difficulty in rooting.

Inactive Publication Date: 2016-06-15
BAODING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Experiments have shown that this method of inducing rooting can effectively solve the problem of difficult root

Method used

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  • Root induction method for Chinese fir test-tube plantlets
  • Root induction method for Chinese fir test-tube plantlets
  • Root induction method for Chinese fir test-tube plantlets

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Induced rooting technique of Chinese fir test-tube seedlings

[0024] Experimental materials The materials come from the aseptic seedlings of Chinese fir in the state-owned forest farm of Laibin City, Guangxi, and have undergone 5 consecutive multiplication and cultivation of cluster buds.

[0025] Experimental equipment Ultra-clean workbench, tweezers, scalpel, sterile filter paper and medium containing two different hormones and blank medium. The whole experiment process was carried out in the cultivation room, the temperature was 24±1°C, and the light intensity was 2000-3000LX.

[0026] The Chinese fir test-tube seedling material treatment first selects the Chinese fir test-tube seedlings that grow robustly and has a size of 3.0-4.0 cm, and then cuts off 2-3 leaves at the base of the test-tube seedlings when inoculating (it is advisable that the leaves do not contact the medium when inoculating). During the inoculation process, do not leave the fir test-tube seedlin...

Embodiment 2

[0036] Effects of seedling hardening time on rooting of Chinese fir tissue culture seedlings on light substrate nutrient cups

[0037] High-concentration auxin stimulates and induces the rooting of Chinese fir test-tube seedlings with the same steps as in Example 1, and adopts the Chinese fir test-tube seedlings inoculated into the 2,4-D naphthalene acetic acid NAA auxin solution of 10 mg / L in embodiment 1 after stimulating treatment for 3d The test-tube seedlings were inserted into a blank medium and cultivated for 1 day (the blank medium specifically consists of the following raw materials in parts by weight: 50 g of MS medium, 3 g of diatom powder, 2.4 g of sucrose, and 0.25 g of konjac glucomannan. ), then this Chinese fir test-tube plantlet is processed 24h in the 2,4-D naphthalene acetic acid NAA auxin solution of 10mg / L, and the induced rooting Chinese fir test-tube plantlet that obtains carries out the next test:

[0038] Cultivate the rooted test-tube seedlings under ...

Embodiment 3

[0043] Effects of fly ash, sawdust and thiourea on the rooting of fir tissue culture seedlings on light substrate nutrient cups

[0044] High-concentration auxin stimulates and induces the rooting of Chinese fir test-tube seedlings with the same steps as in Example 1, and adopts the Chinese fir test-tube seedlings inoculated into the 2,4-D naphthalene acetic acid NAA auxin solution of 10 mg / L in embodiment 1 after stimulating treatment for 3d The test-tube seedlings were inserted into a blank medium and cultivated for 1 day (the blank medium specifically consists of the following raw materials in parts by weight: 50 g of MS medium, 3 g of diatom powder, 2.4 g of sucrose, and 0.25 g of konjac glucomannan. ), then this Chinese fir test-tube plantlet is processed 24h in the 2,4-D naphthalene acetic acid NAA auxin solution of 10mg / L, and the induced rooting Chinese fir test-tube plantlet that obtains carries out the next test:

[0045] The rooted test-tube seedlings were cultivate...

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Abstract

The invention provides a root induction method for Chinese fir test-tube plantlets. The method specifically includes the steps that the robust Chinese fir test-tube plantlets are inoculated to high-concentration auxin to be stimulated and induced to root and then are inoculated to an NAA auxin solution, the test-tube plantlets obtained after stimulation treatment are inoculated into a blank culture medium to be cultured for 1 day, the Chinese fir test-tube plantlets are treated in the NAA auxin solution and then are cultured at the constant temperature of 25 DEG C under LED red light, and root induction culture of the Chinese fir test-tube plantlets can be completed; the Chinese fir test-tube plantlets obtained after root induction culture are transplanted into a greenhouse and are hardened at the room temperature; the base parts of the Chinese fir tissue-cultured plantlets are treated with ABT1+IAA+yellow mud after the culture medium is cleaned away, then the hardened Chinese fir plantlets are transplanted to a light-medium culture cup, and rooting treatment of the Chinese fir test-tube plantlets is completed. According to the method, after stimulation treatment is carried out with high-concentration auxin ANN, root source bases can be easily formed; then, the Chinese fir test-tube plantlets are transferred into the blank culture medium, so that elongation growth of the root source bases is promoted; the rooting percentage is increased to 95.8% from 10%.

Description

technical field [0001] The invention relates to a plant induction rooting culture, in particular to a Chinese fir plant induction rooting culture. Background technique [0002] Chinese fir (Cunninghamialanceolata) is a plant of the genus Cunninghamia in the family Taxodiaceae. Because Chinese fir is a cross-pollination tree species, it is propagated by seeds, and the individual differentiation is large, so it is difficult to maintain the traits of the female parent of Chinese fir. Adopting the method of tissue culture can not only maintain the excellent traits of the mother body, but also can produce on a large scale. However, the existing Chinese fir tissue culture seedlings are raised on light substrate nutrient cups, which show problems such as low rooting rate, easy root breakage when transplanting, complicated rooting procedures, and high rooting costs, which have always hindered the production process of Chinese fir factory seedlings. Therefore develop a kind of Chin...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001
Inventor 蒋凡
Owner BAODING UNIV
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