A kind of method of tall fescue tissue culture and differentiation regeneration

Abstract

The present invention proposes a method for tall fescue tissue culture and differentiation regeneration, comprising the following steps: S1, disinfection and sterilization of seeds; S2, induction and culture of explant embryogenic callus; S3, callus proliferation and culture ; S4, differentiation culture of embryogenic callus and rapid proliferation of immature embryos; S5, rooting culture of strong seedlings and transplanting of sterile seedlings. The invention has simple operation, and the seed germ can easily form embryogenic callus, and a complete plant with roots, stems and leaves can be formed on the differentiation medium, and the transplanted survival rate of the formed tissue culture seedlings is high. After embryoid body proliferation and culture, a large number of embryoid body cluster buds or plantlets can be formed within a short period of 2 months. Cluster buds or plantlets only need about 20 days of cultivation in minimal medium to form complete robust seedlings with roots, stems and leaves.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to a method for tissue culture and differentiation and regeneration of tall fescue. Background technique [0002] Tall fescue (Festuca elata Keng ex E. Alexeev) is a perennial herb of the subgenus Fescue of the Poaceae family. It is a perennial herb because of its strong adaptability, outstanding stress resistance, strong resistance to trampling and disease, and no dormancy in summer. Grass species suitable for widespread promotion and use are indispensable landscaping lawn ground cover plants in cool-season landscaping. [0003] It is very difficult to propagate tall fescue through seeds, so ramet propagation is often used in production. However, ramet propagation not only has a very low reproduction coefficient, but also slows growth. Rapid propagation and popularization of germplasm. SUMMARY OF THE INVENTION [0004] The technical problem to be solved by the present inventi...

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Problems solved by technology

[0003] It is very difficult to propagate tall fescue through seeds, so it is often used in production to propagate by division. However, division propagation not only has a low reproduction coefficient, but also slow growth, and it is easy to cause serious decline in germplasm, which affects the quality of tall fescue. Rapid Propagation and Application of Germplasm

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