A method for constructing peanut cotyledon regeneration system

A technology of peanut cotyledon and system, applied in the field of plant tissue culture, can solve the problems of poor rooting quality and few fibrous roots, and achieve the effect of promoting the formation of embryogenic callus

Active Publication Date: 2021-04-09
HUAIBEI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, Hao Haoyong et al. (2007) reported that clustered buds were in 1/2 MS+NAA1.0mg.L -1 The rooting rate on +0.7% agar medium is up to 95%, but according

Method used

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  • A method for constructing peanut cotyledon regeneration system
  • A method for constructing peanut cotyledon regeneration system
  • A method for constructing peanut cotyledon regeneration system

Examples

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Embodiment 1

[0035] A method for constructing a peanut cotyledon regeneration system, specifically comprising the following steps:

[0036] (1) Pretreatment: select mature "Si Li Hong" peanut seeds and soak them in sterile water for 2 hours;

[0037] (2) Sterilization: Soak the pretreated seeds in 75% ethanol solution for 30s, then use 0.1% HgCl 2 Disinfect the solution for 8 minutes, rinse with sterile water 4 times, 4 minutes each time;

[0038] (3) Callus culture: peel off the germ from the sterilized seeds, cut the cotyledon from the middle and inoculate it on the callus induction medium, irradiate it with a white fluorescent lamp, and supplement it with red light, and then cover it with two layers of newspapers to make Peanut cotyledons receive an illumination of 500 lux; at 25°C, cultivate for 20 days with 16 hours of light per day and 8 hours of darkness;

[0039] Among them, the intensity of white light and red light before covering with newspapers are both 1500lux;

[0040] The...

Embodiment 2

[0048] A method for constructing a peanut cotyledon regeneration system, specifically comprising the following steps:

[0049] (1) Pretreatment: select mature "Si Li Hong" peanut seeds and soak them in sterile water for 4 hours;

[0050] (2) Sterilization: Soak the pretreated seeds in 75% ethanol solution for 30s, then use 0.1% HgCl 2 Disinfect the solution for 10 minutes, rinse with sterile water 5 times, 5 minutes each time;

[0051] (3) Callus culture: the sterilized seeds are stripped of the germ, and the cotyledons are cut from the middle and inoculated on the callus induction medium. The components of the callus induction medium are: MS+2mg / L TDZ+ 0.8mg / L 6-BA+1mg / L NAA, irradiated by white fluorescent lamps, supplemented by red light, and covered with two layers of newspapers, so that the illuminance received by peanut cotyledons is 1000lux; Cultivate in dark for 8 hours and cultivate for 20 days;

[0052] Among them, the intensity of white light and red light before...

Embodiment 3

[0059] A method for constructing a peanut cotyledon regeneration system, specifically comprising the following steps:

[0060] (1) Pretreatment: select mature "Si Li Hong" peanut seeds and soak them in sterile water for 3 hours;

[0061] (2) Sterilization: Soak the pretreated seeds in 75% ethanol solution for 30s, then use 0.1% HgCl 2 Disinfect the solution for 9 minutes, rinse with sterile water 5 times, 4 minutes each time;

[0062] (3) Callus culture: the sterilized seeds are stripped of the germ, such as figure 1 As shown, the cotyledons were cut from the middle and inoculated on the callus induction medium. The components of the callus induction medium were: MS+2mg / L TDZ+0.8mg / L 6-BA+1mg / L NAA, white Irradiated by fluorescent lamps, supplemented with red light, and covered with two layers of newspapers, so that the illuminance received by the peanut cotyledons was 800 lux; under the condition of 25°C, cultivated for 20 days with 16 hours of light and 8 hours of darkness...

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Abstract

The invention relates to the technical field of plant tissue culture, and specifically discloses a method for constructing a peanut cotyledon regeneration system, comprising the following steps: (1) pretreatment; (2) sterilization; (3) callus culture; (4) Cluster bud induction; (5) Proliferation culture; (6) Strong seedling culture; (7) Rooting culture; (8) Transplanting. The method for constructing a stable peanut cotyledon regeneration system provided by the invention provides a new technology and method for peanut genetic transformation and molecular breeding.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, and specifically discloses a method for constructing a peanut cotyledon regeneration system. Background technique [0002] Peanut is an annual herbaceous leguminous crop, one of the main raw material varieties of high-quality edible oil, also known as "longevity fruit" among the people. As an important crop in my country, peanut not only has rich nutritional value, but also has high economic value. [0003] Plant tissue culture technology is the basis for research on peanut variety improvement, new variety propagation, genetic transformation, germplasm preservation and screening of resistant mutants. In recent years, there have been many reports on obtaining regenerated plants by in vitro culture of peanut immature embryos, cotyledons, stem tips, hypocotyls, leaves, pollen, etc. as explants. However, the establishment of peanut regeneration system is still immature, and it is diffi...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 余如刚杜雪玲宋运贤王国良杨星蒋晓晓陈涛李静管方园刘兰梅雪珂
Owner HUAIBEI NORMAL UNIVERSITY
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