Festuca arundinacea tissue culture and differentiation regeneration method

A technique of tissue culture and tall fescue, which is applied in the field of genetic engineering, can solve the problems of low reproductive coefficient, slow growth, and germplasm decline of ramets, and achieve the effects of easy transplanting and survival, simple operation, and solving difficult reproduction

Active Publication Date: 2021-04-09
南京栢特隆生物科技有限公司
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Problems solved by technology

[0003] It is very difficult to propagate tall fescue through seeds, so it is often used in production to propagate by division. However, division propagation not only has a low reproduction coefficient, but also slow growth, and it is easy to cause serious decline in germplasm, which affects the quality of tall fescue. Rapid Propagation and Application of Germplasm

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  • Festuca arundinacea tissue culture and differentiation regeneration method
  • Festuca arundinacea tissue culture and differentiation regeneration method
  • Festuca arundinacea tissue culture and differentiation regeneration method

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Embodiment Construction

[0018] It is easy to understand that, according to the technical solution of the present invention, those skilled in the art can propose multiple structural modes and implementation modes that can be replaced without changing the essence and spirit of the present invention. Therefore, the following specific embodiments are only exemplary descriptions of the technical solution of the present invention, and should not be regarded as the entirety of the present invention or as a limitation or limitation on the technical solution of the present invention.

[0019] A method for tall fescue tissue culture and differentiation regeneration, specifically comprising the steps of:

[0020] S1. Disinfection and sterilization of seeds: soak in 50% sulfuric acid for 25 minutes, rinse with sterile water to remove isolated glumes and lemmas, then disinfect with 70% alcohol for 45 seconds, rinse with sterile water for 3 times, and finally rinse with 30% alcohol. % sodium hypochlorite (availabl...

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Abstract

The invention provides a festuca arundinacea tissue culture and differentiation regeneration method. The method comprises the following steps of S1, disinfecting and sterilizing seeds; S2, carrying out induction culture on embryonic calluses of explants; S3, carrying out proliferation culture on the calluses; S4, performing differentiation culture of the embryogenic calluses and rapid proliferation of immature embryos; and S5, performing strong seedling rooting culture and aseptic seedling transplanting. The method is simple to operate, the embryogenic calluses are easily formed by seed germs, complete plants with rhizomes and leaves are formed on a differential medium, and formed tissue culture seedlings are high in transplanting survival rate. Through embryoid multiplication culture, a large number of embryoid cluster buds or plantlets can be formed in a short period of 2 months. The cluster buds or plantlets only need to be cultured in a basic culture medium for about 20 days to form complete robust seedlings with roots, stems and leaves.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to a method for tissue culture, differentiation and regeneration of tall fescue. Background technique [0002] Tall fescue (Festuca elata Keng ex E.Alexeev) is a perennial herb of the Gramineae subgenus Fescue. Grass species suitable for widespread promotion and use are indispensable lawn ground cover plants in cool-season landscaping. [0003] It is very difficult to propagate tall fescue through seeds, so it is often used in production to propagate by division. However, division propagation not only has a low reproduction coefficient, but also slow growth, and it is easy to cause serious decline in germplasm, which affects the quality of tall fescue. Rapid propagation and application of germplasm. Contents of the invention [0004] The technical problem to be solved by the present invention is to overcome the defects of the prior art. The present invention proposes a method ...

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 许磊
Owner 南京栢特隆生物科技有限公司
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