Method for constructing malus asiatica cotyledon regeneration system

A technology of cotyledon and system, which is applied in the directions of plant regeneration, horticultural methods, botanical equipment and methods, etc., can solve the problems of difficult regeneration and difficult rooting, and achieves the effects of robust regenerated buds, many regenerated buds, and easy operation.

Active Publication Date: 2020-06-26
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method of using cotyledons as explants to induce differentiation into roots or buds, and to construct a regeneration system for cotyledons, which overcomes the shortcomings of the original regeneration system and solves the problems of difficult regeneration and rooting.

Method used

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  • Method for constructing malus asiatica cotyledon regeneration system
  • Method for constructing malus asiatica cotyledon regeneration system
  • Method for constructing malus asiatica cotyledon regeneration system

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Embodiment 1

[0039] A method for constructing a red flower cotyledon regeneration system, using the red flower red cotyledons as explants to complete in vitro regeneration, comprising the following steps:

[0040] (1) Pretreatment: select mature and plump flower red seeds and soak them in sterile water for 10 minutes;

[0041] (2) Sterilization: Soak the above-mentioned seeds with 75% alcohol for 30 seconds, rinse them with sterile water for 2-3 times, then soak them with 10% sodium hypochlorite solution for 10 minutes, and rinse them with sterile water for 3-4 times;

[0042] (3) Adventitious bud induction: after the sterilized seeds were peeled off the germ, after cutting off some cotyledons at the proximal end and the distal end, they were inoculated on the adventitious bud induction medium, cultured in the dark for 7 days, and then cultured under light;

[0043] Adventitious bud induction medium components are: MS+1mg / LTDZ+0.05mg / LNAA;

[0044] (4) elongate strong bud cultivation: tra...

Embodiment 2

[0050] A method for constructing a red flower cotyledon regeneration system, using the red flower red cotyledons as explants to complete in vitro regeneration, specifically includes the following steps:

[0051] (1) Pretreatment: select plump red seeds and soak them in sterile water for 10 minutes;

[0052] (2) Sterilization: wash with 75% alcohol for 30 seconds, wash with sterile water for 2-3 times, then treat with 10% sodium hypochlorite solution for 10 minutes, and wash with sterile water for 3-4 times;

[0053] (3) Adventitious bud induction: the sterilized seeds are stripped of the germ, cut off some cotyledons at the proximal end and the distal end, and inoculated on the adventitious bud induction medium, and cultivated under light;

[0054] Adventitious bud induction medium components are: MS+1mg / LTDZ+0.05mg / LNAA;

[0055](4) elongate strong bud cultivation: transfer step (3) culture material to elongate strong bud culture medium, cultivate under light for 30-40 days;...

Embodiment 3

[0061] A method for constructing a red flower cotyledon regeneration system, using the red flower red cotyledons as explants to complete in vitro regeneration, specifically includes the following steps:

[0062] (1) Pretreatment: select plump red seeds and soak them in sterile water for 10 minutes;

[0063] (2) Sterilization: wash with 75% alcohol for 30 seconds, wash with sterile water for 2-3 times, then treat with 10% sodium hypochlorite solution for 10 minutes, and wash with sterile water for 3-4 times;

[0064] (3) Adventitious bud induction: the sterilized seeds are stripped of the germ, cut off some cotyledons at the proximal end and the distal end, and inoculated on the adventitious bud induction medium, cultured in the dark for 14 days, and then cultured under light;

[0065] Adventitious bud induction medium components are: MS+1mg / LTDZ+0.05mg / LNAA;

[0066] (4) elongate strong bud cultivation: transfer step (3) culture material to elongate strong bud culture medium,...

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Abstract

The invention discloses a method for constructing a malus asiatica cotyledon regeneration system. The method comprises the following steps of I, performing pretreatment; II, performing sterilization;III, inducing adventitious buds; IV, performing extending for strengthening buds and performing culturing; V, performing rooting culturing; and IV, performing transplanting. Malus asiatica cotyledon is used as an explant, in vitro regeneration is completed, and the technical solution provided by the invention is used for in vitro regeneration of malus asiatica, so that the induction sprouting rateand the value increment coefficient are increased. Experiment prove that obtained regrowth seedlings have the advantages that buds are strong, main roots are healthy, and seedling transplanting survival rate is high. The method for constructing a malus asiatica cotyledon regeneration system provided by the invention provides a new technique and method for malus asiatica heredity transformation and molecular breeding.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for constructing a red cotyledon regeneration system. Background technique [0002] Flower red (Malus asiatica), Rosaceae (Rosaceae), Malus (Malus), deciduous small trees. Huahong, also known as "Shaguo" and "Lin'ao", is flat in nature, sweet and sour in taste, rich in various nutrients, and has the effects of quenching thirst, promoting body fluid, and eliminating stagnation. In addition to eating fresh, the fruit can also be processed into dried fruit, fruit skin or wine. Huahong is not only an ornamental tree species with beautiful flowers and fruits, but also an excellent rootstock for apples because of its good grafting affinity with apples. However, Huahong is mainly propagated by grafting, and the severe deforestation has led to a serious shortage of germplasm resources in Huahong. This problem can be solved by establishing a regeneratio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 文晓鹏邹思艳田文杰李孝绒
Owner GUIZHOU UNIV
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