Method for constructing malus asiatica cotyledon regeneration system
A technology of cotyledon and system, which is applied in the directions of plant regeneration, horticultural methods, botanical equipment and methods, etc., can solve the problems of difficult regeneration and difficult rooting, and achieves the effects of robust regenerated buds, many regenerated buds, and easy operation.
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Embodiment 1
[0039] A method for constructing a red flower cotyledon regeneration system, using the red flower red cotyledons as explants to complete in vitro regeneration, comprising the following steps:
[0040] (1) Pretreatment: select mature and plump flower red seeds and soak them in sterile water for 10 minutes;
[0041] (2) Sterilization: Soak the above-mentioned seeds with 75% alcohol for 30 seconds, rinse them with sterile water for 2-3 times, then soak them with 10% sodium hypochlorite solution for 10 minutes, and rinse them with sterile water for 3-4 times;
[0042] (3) Adventitious bud induction: after the sterilized seeds were peeled off the germ, after cutting off some cotyledons at the proximal end and the distal end, they were inoculated on the adventitious bud induction medium, cultured in the dark for 7 days, and then cultured under light;
[0043] Adventitious bud induction medium components are: MS+1mg / LTDZ+0.05mg / LNAA;
[0044] (4) elongate strong bud cultivation: tra...
Embodiment 2
[0050] A method for constructing a red flower cotyledon regeneration system, using the red flower red cotyledons as explants to complete in vitro regeneration, specifically includes the following steps:
[0051] (1) Pretreatment: select plump red seeds and soak them in sterile water for 10 minutes;
[0052] (2) Sterilization: wash with 75% alcohol for 30 seconds, wash with sterile water for 2-3 times, then treat with 10% sodium hypochlorite solution for 10 minutes, and wash with sterile water for 3-4 times;
[0053] (3) Adventitious bud induction: the sterilized seeds are stripped of the germ, cut off some cotyledons at the proximal end and the distal end, and inoculated on the adventitious bud induction medium, and cultivated under light;
[0054] Adventitious bud induction medium components are: MS+1mg / LTDZ+0.05mg / LNAA;
[0055](4) elongate strong bud cultivation: transfer step (3) culture material to elongate strong bud culture medium, cultivate under light for 30-40 days;...
Embodiment 3
[0061] A method for constructing a red flower cotyledon regeneration system, using the red flower red cotyledons as explants to complete in vitro regeneration, specifically includes the following steps:
[0062] (1) Pretreatment: select plump red seeds and soak them in sterile water for 10 minutes;
[0063] (2) Sterilization: wash with 75% alcohol for 30 seconds, wash with sterile water for 2-3 times, then treat with 10% sodium hypochlorite solution for 10 minutes, and wash with sterile water for 3-4 times;
[0064] (3) Adventitious bud induction: the sterilized seeds are stripped of the germ, cut off some cotyledons at the proximal end and the distal end, and inoculated on the adventitious bud induction medium, cultured in the dark for 14 days, and then cultured under light;
[0065] Adventitious bud induction medium components are: MS+1mg / LTDZ+0.05mg / LNAA;
[0066] (4) elongate strong bud cultivation: transfer step (3) culture material to elongate strong bud culture medium,...
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