Acer palmatum seed tissue culture method

A technology of tissue culture and maple, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of seasonal restrictions, low cutting propagation coefficient, long seedling breeding period, etc., and overcome seasonal limitations. , The effect of convenient material acquisition and convenient operation

Active Publication Date: 2015-03-25
SICHUAN COLORLINK CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method overcomes the disadvantages of long conventional seedling cultivation period of Acer palmatum and low cutting propagation coefficient, and realizes the factory production of Acer palmatum seedlings, the method uses stems as explants, and the materials are limited by seasons, and it is inconvenient

Method used

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  • Acer palmatum seed tissue culture method
  • Acer palmatum seed tissue culture method
  • Acer palmatum seed tissue culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: A kind of Acer palmatum seed tissue culture method, it comprises the following steps:

[0021] S1. Seed storage: Collect mature seeds of Acer palmatum in October every year, mix the seeds with sand, the mixing volume is 1:3, the humidity of the sand is such that it will form a ball without dripping, and it will crack when loosened. Store in a refrigerator at 4°C;

[0022] S2. Seed disinfection: Take out the stored seeds, wash them in running water for 5 minutes, peel off the seed shells with a scalpel and tweezers in an ultra-clean workbench, treat them with 75% alcohol for 15 seconds, and then put them in 0.1% mercuric chloride for 5 minutes , washed with sterile water 4 times;

[0023] S3. Start culture: inoculate the sterilized seeds in the start medium for cultivation, wherein the start medium is 1 / 2MS+GA 3 0.5mg / L + 30g / L sucrose + 5g / L agar, the pH value is 6, the culture conditions are: light intensity 2800LX, light 14h, temperature 22°C, culture ...

Embodiment 2

[0026] Example 2: A kind of Acer palmatum seed tissue culture method, it comprises the following steps:

[0027] S1. Seed storage: Collect mature seeds of Acer palmatum in October every year, mix the seeds with sand, the mixing volume is 1:3, the humidity of the sand is such that it will form a ball without dripping, and it will crack when loosened. Store in a refrigerator at 4°C;

[0028] S2. Seed disinfection: Take out the stored seeds, rinse them in running water for 10 minutes, use a scalpel and tweezers to peel off the seed shells in an ultra-clean workbench, treat them with 75% alcohol for 20 seconds, and then put them in 0.1% mercuric chloride for 10 minutes , washed with sterile water 6 times;

[0029] S3. Start culture: inoculate the sterilized seeds in the start medium for cultivation, wherein the start medium is 1 / 2MS+GA 3 0.5mg / L + 30g / L sucrose + 5g / L agar, the pH value is 7, the culture conditions are: light intensity 3200LX, light 18h, temperature 26°C, cult...

Embodiment 3

[0032] Example 3: A kind of Acer palmatum seed tissue culture method, it comprises the following steps:

[0033] S1. Seed storage: Collect mature seeds of Acer palmatum in October every year, mix the seeds with sand, the mixing volume is 1:3, the humidity of the sand is such that it will form a ball without dripping, and it will crack when loosened. Store in a refrigerator at 4°C;

[0034] S2. Seed disinfection: take out the stored seeds, rinse them in running water for 8 minutes, use a scalpel and tweezers to peel off the seed shells in an ultra-clean workbench, treat them with 75% alcohol for 18 seconds, and then put them in 0.1% mercuric chloride for 7 minutes , washed 5 times with sterile water;

[0035] S3. Start culture: inoculate the sterilized seeds in the start medium for cultivation, wherein the start medium is 1 / 2MS+GA 3 0.5mg / L + sucrose 30g / L + agar 5g / L, the pH value is 7, the culture conditions are: light intensity 3000LX, light 15h, temperature 24°C, culture ...

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Abstract

The invention discloses an acer paimatum seed tissue culture method. The acer paimatum seed tissue culture method comprises the following steps: seed storage: ripe acer paimatum seeds are collected in October every year, mixed with sand, and placed and stored in a refrigerator at a temperature of 4 DEG C; seed sterilization: the stored seeds are taken out and flushed, the casings of the seeds are peeled off, and the peeled seeds are sterilized with alcohol and mercuric chloride; initiation culture: an initiation culture medium comprises 1 / 2MS, 30.5 mg / L of GA, 30g / L of cane sugar and 5g / L of agar, and the pH value of the initiation culture medium is 6-7; primary culture: budded seeds are inoculated to a primary culture medium for culture, the primary culture medium is provided with 1 / 2 MS, 30.5mg / L of GA, 0.05mg / L of NAA, 30g / L of cane sugar and 5g / L of agar, and the pH value of the primary culture medium is 6-7; subculture: after tissue culture seedlings subjected to the primary culture are cut, each section of the tissue culture seedling has 2-5 axillary buds and is transferred to a subculture medium for culture, the subculture medium is provided with 1 / 2MS, 0.1mg / L of TDZ, 0.05mg / L of KT, 30g / L of cane sugar and 5g / L of agar, and the pH value of the subculture medium is 6-7. The acer paimatum seed tissue culture method is simple in culture process and convenient to operate, and provides technique support for industrial acer paimatum seedling-raising.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for tissue culture of Acer palmatum seeds. Background technique [0002] Acer paimatum (Acer paimatum), also known as Yafeng, belongs to the genus Aceraceae, and is a valuable ornamental tree species in urban landscapes. It is native to the Yangtze River Basin in China, and is also distributed in Japan and the Korean Peninsula. my country is mainly distributed in the provinces of the Yangtze River Basin, and it mostly grows in forest margins or sparse forests of mountains and hills below 1200m above sea level. Acer palmatum is a deciduous small tree, about 8m high, with dark gray bark, purple or lavender green branches in the current year; light gray, purple or dark purple branches in perennial branches. Acer palmatum leaves opposite, 7-10 cm in diameter, truncate at base, deeply lobed palmately, with 5-9 lobes, usually 7-lobed, lobes ovate-oblong or lanceolat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 冯爱云王小辉吴佳川沈香兰朱晓菲
Owner SICHUAN COLORLINK CO LTD
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