A kind of peony tissue culture container seedling technology

Abstract

The invention belongs to the field of tissue culture of plants, and particularly relates to a peony tissue culture container seedling method. The method comprises the following steps: collecting healthy and full bulbil without pests or diseases as explants from robust mother plants of Paeonia ostii, Mingxing and other peony varieties, performing surface sterilization with an environment-friendly explant surface sterilization method, and inoculating the explant onto a specially prepared poeny culture medium to establish a sterile culture system; performing subculture multiplication of test-tube plantlets on the multiplication medium with a subculture period of 28-35 days; performing induced rooting of the test-tube plantlets on a nutrition bowl medium in a culture bottle to form plantlet container seedlings; and performing environment adaptability acclimatization to form tissue culture container seedlings which are suitable for natural environment and can be repotted, transplanted and taken out of flower bed for application anytime and anywhere. The method has the main characteristics of the environment-friendly poeny explant surface sterilization method, a basis medium for tissue culture of peony, and a tissue culture medium and a culture method of peony container seedlings, which are key technology for factory-like breeding of high quality seedlings of peony.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, and relates to a tissue culture propagation method of peony. Specifically, the present invention relates to a method of collecting healthy and plump scale buds free from diseases and insect pests from a vigorously growing peony mother plant as an explant, and establishing an aseptic culture system with an environmentally friendly surface disinfection method; in a WPL value-added medium Expansion of test-tube plantlets (adventitious buds) on the top; in the nutrient pot rooting matrix in the tissue culture bottle, the test-tube plantlets are induced to take root to form tissue culture container plantlets, and through the process of environmental adaptation (transition and hardening), the tissue culture container plantlets are adapted The plant tissue culture room (referred to as "tissue culture room", the same below) is conditionally domesticated into container seedlings adapted to the natural co...

AI Technical Summary

Problems solved by technology

[0005] The main problems in peony tissue culture are: (1) It is difficult to sterilize the surface of explants (scale buds), and the highly toxic disinfectant mercuric chloride has to be used, and its residue will have an impact on the environment, operators and culture materials; (2) ) adventitious buds are easy to brown and even die on the medium;

Small plants (rooted test-tube plantlets) are prone to death during the transition from the laboratory environment to the field environment, resulting in peony tissue culture cannot be used for production

[0006] Peony sowing seedlings and seedling transplanting are severely restricted by the season, and must be carried out in autumn, otherwise the seeds will sleep in the soil and cannot germinate and grow, and the land resources will be wasted until the next spring; transplanting must be in autumn, otherwise most of the plants will die, which also limits Peony Industry Development