Peony tissue culture container seedling technology
A technology of tissue culture and container seedlings, applied in the field of plant tissue culture, can solve the problems that seeds cannot germinate, grow, die, and will be affected
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Embodiment 1
[0020] Establishment of Aseptic Culture System of Peony
[0021] Peony 'Fengdan' (PaenoiaosttiT.HongetJ.X.Zhangvar.LishizheniiB.A.SheninActaPhytota.Sin.1997,35(4):360-361),'Star'(PaenoiasuffruticosaAndr.'Mingxing'),'Wen Gonghong'( PaenoiasuffruticosaAndr.'Wengonghong') etc., select robust plants, collect healthy and plump scale buds without damage by diseases and insect pests as explants in October of that year-March of the next year, and dip in baby bath soap to clean the surface with a soft hairbrush. Soak cresol (trade name: Dettol) aqueous solution (35-50ml per liter of chlorine-containing m-xylenol) for 5-10min (during gentle shaking), rinse with running water for 20min, add 28% hydrogen peroxide and 70 % ethanol mixture (3:1, volume ratio), drip into Tuwen-80 (0.2ml per 50ml mixture is 1 drop), sterilize for 8-15min, rinse with sterile water 3-4 times, every 3 minutes each time. Put the scale buds on sterilized filter paper to drain the surface water, peel off the scal...
Embodiment 2
[0023] Tissue Culture Propagation of Peony Test-tube Seedlings (Adventitious Buds)
[0024] Preferred peony 'Feng Dan' (PaeoniaostiiT.HongetJ.X.Zhangvar.lishizheniiB.A.Shen), 'star' (PaenoiasuffruticosaAndr.'Mingxing') plants, in January, gather healthy and plump scale buds as described in Example 1 Methods A sterile culture system was established; axillary buds were cultured on WPL growth medium (medium formula and preparation method were the same as above), and cultured in a tissue culture room with a temperature of 23-25°C and a light of 36 μmol / m 2 / s, the daylight rhythm is 16h / 8h (light / dark), and the air is ventilated by cellulose acetate membrane filtration. The cultures were grown under these conditions for 28 days, and each culture differentiated 2-5 adventitious buds. The theoretical reproductive quantity is calculated according to the formula y=x n (y is the reproduction quantity, x is the value-added multiple, and n is the average number of times of value-added ...
Embodiment 3
[0027] Peony tissue culture container seedlings
[0028] Peony 'Fengdan' (PaenoiaosttiT.HongetJ.X.Zhangvar.LishizheniiB.A.Shen) obtained test-tube plantlets according to the above-mentioned embodiment one and two. The test-tube seedlings are cultivated into tissue culture container seedlings adapted to the field environment through the following three stages. (1) Container seedling culture, test-tube seedlings take root to form container seedlings through the following cultivation process: rooting mixed substrate [charcoal (particle size ≤ 5mm) or perlite and peat (2:1, v:v), activated carbon 1g / L] Put into a black polyethylene nutrition bowl (3-4.5cm×3-4.5cm, diameter×height), put the nutrition bowl into a transparent tissue culture bottle (about 7.5cm×10cm, diameter×height); add rooting culture solution into the culture bottle 40ml [each liter contains 1 / 3-1 / 2WPL inorganic substances, indole butyric acid 1-3mg, and the rest is water]; high-temperature sterilization (tempera...
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