A kind of tissue culture rapid propagation method of Chongyang wood

A technology of tissue culture rapid propagation and Chongyang wood, which is applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., to achieve the effects of shortening the breeding cycle, low cultivation cost, and simple operation

Active Publication Date: 2022-03-25
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] After reviewing the literature, it is found that scholars' research on Chongyang tree mainly focuses on the cultivation technology of Chongyang tree and the technology of disease and insect pest control, but there is no report on the complete tissue culture and rapid propagation technology of Chongyang tree

Method used

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  • A kind of tissue culture rapid propagation method of Chongyang wood
  • A kind of tissue culture rapid propagation method of Chongyang wood
  • A kind of tissue culture rapid propagation method of Chongyang wood

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Embodiment 1

[0040] A kind of tissue culture rapid propagation method of Chongyang wood of the present embodiment, with the Chongyang wood axillary bud stem section as explant, comprises the following steps:

[0041] A1. Acquisition and disinfection of explants: The experimental materials come from excellent individual plants with strong flooding resistance in Xinguanzhou Forest Farm, Anqing City, Anhui Province; the freshly picked axillary bud stems of Chongyang wood were rinsed with washing powder for 1min and running water Rinse for 1 hour, then use 75% alcohol to rinse quickly for 30 seconds in an ultra-clean workbench, rinse with sterile water for 4 times, soak in 1% sodium hypochlorite for 5 minutes, and finally, rinse with sterile water for 6 times; Drain the moisture from the leaves.

[0042] A2. Induction culture: Cut the stem section of the axillary buds of Chongyang chrysanthemum after absorbing the water into 1cm under sterile conditions 2 Cut into pieces of different sizes, a...

Embodiment 2

[0046] A kind of tissue culture rapid propagation method of Chongyang wood of the present embodiment, with the Chongyang wood axillary bud stem section as explant, comprises the following steps:

[0047] A1. Acquisition and disinfection of explants: the experimental materials come from excellent individual plants with strong flooding resistance in Xinguanzhou Forest Farm, Anqing City, Anhui Province; the freshly picked axillary bud stems of Chongyang scallopedifolia were rinsed with washing powder for 0.5min, Rinse with running water for 0.5h, then use 75% alcohol to rinse quickly for 25s, rinse with sterile water for 3 times, soak in 1% sodium hypochlorite for 4min in the ultra-clean workbench, and finally, rinse with sterile water for 5 times; Absorbent paper absorbs the moisture on the leaves for later use.

[0048] A2. Induction culture: Cut the stem section of the axillary buds of Chongyang chrysanthemum after absorbing the water into 1cm under sterile conditions 2 Cut i...

Embodiment 3

[0052] A kind of tissue culture rapid propagation method of Chongyang wood of the present embodiment, with the Chongyang wood axillary bud stem section as explant, comprises the following steps:

[0053] A1. Acquisition and disinfection of explants: the experimental materials come from excellent individual plants with strong flooding resistance in Xinguanzhou Forest Farm, Anqing City, Anhui Province; freshly picked axillary bud stems of S. chrysalis Chongyang are rinsed with washing powder for 1.5min, Rinse with running water for 1.5 hours, then use 75% alcohol to rinse quickly for 35 seconds in an ultra-clean workbench, rinse with sterile water for 5 times, soak in 1% sodium hypochlorite for 6 minutes, and finally rinse with sterile water for 7 times; Absorbent paper absorbs the moisture on the leaves for later use.

[0054] A2. Induction culture: Cut the stem section of the axillary buds of Chongyang chrysanthemum after absorbing the water into 1cm under sterile conditions ...

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Abstract

The invention provides a method for tissue culture and fast propagation of Chongyang wood, which uses Chongyang wood with axillary bud stem segments or Chongyang wood leaves as explants for tissue culture propagation. When the explants are Chongyang wood with axillary bud stem segments, the cultivation method is: sterilize the bud stem segments, then place the bud stem segments in the induction medium to directly obtain clump seedlings, and then cut the clump seedlings into individual seedlings and inoculate them one by one. Rooting culture is carried out on the rooting medium, and finally the seedlings after rooting and culture are refined and transplanted in sequence, so as to realize the rapid propagation of Chongyang tree. When the sterile leaves of Chongyang wood are used as explants, the culture method is as follows: sterilize Chongyang wood seeds, and cultivate the sterile leaves, then use the sterile leaves as the explants, and sequentially induce callus. , Callus differentiation, rooting culture process, the establishment of Chongyang wood tissue culture rapid propagation system. The invention has the advantages of simple operation, low cultivation cost, high reproduction efficiency, little influence by natural environment, and good market and industrial application prospect.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, in particular to a method for tissue culture and rapid propagation of Chongyang wood. Background technique [0002] Bischofia polycarpa (scientific name: Bischofia polycarpa) is a deciduous tree of the genus Bischofia in the family Euphorbiaceae. It is a native tree species in my country and a famous garden ornamental tree species in China. Chongyang wood has the advantages of shade tolerance, loose soil requirements, water and humidity resistance, well-developed root system, and strong wind resistance. It is often distributed in the Qinling Mountains, south of the Huaihe River Basin, the middle and lower reaches of the Yangtze River, and some water network areas. Chongyang wood has a medium growth rate, luxuriant leaves, magnificent tree appearance, and red leaves in autumn. It is usually cultivated as a street tree and ornamental tree in gardens, and it is widely used in landscaping. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 项艳王雅梅王瑞佳何婷
Owner ANHUI AGRICULTURAL UNIVERSITY
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