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A kind of method and application of propagating Atractylodes japonica seedlings by using cluster buds of Atractylodes japonica

An Atractylodes macrocephala and seed technology, applied in the field of medicinal material seedling propagation, can solve the problems of decreased yield and quality of medicinal materials, unstable chemical composition content, inability to meet market demand, etc., and achieve shortened breeding years, good quality and high purity. Effect

Active Publication Date: 2022-07-26
ACAD OF AGRI SCI ENSHI TUJIA MIAOAUTONOMOUS PREFECTURE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] According to the cultivation status of Chinese herbal medicines in my country, during the long-term production process of Atractylodes macrocephala, only planting but not selection, the varieties degenerate, the yield and quality of medicinal materials decline year by year, and the content of chemical components is unstable, which cannot meet the market demand.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Select and select the dominant population of Atractylodes japonica, excellent single plant, robust and young Atractylodes japonica seedlings in the sterile culture system as explants, and prune the explants: according to the size of the explants and the number of axillary buds, prune them into 1-2.5 cm long axillary buds with one leaf and one bud, each plant can be trimmed into 3-5 small axillary buds and placed in a petri dish.

[0050] Propagate Atractylodes seedlings as follows:

[0051] 1. Based on the preserved Atractylodes atractylodes tissue culture seedlings as the propagation material, induce the differentiation of cluster buds: Use autoclaved medical tweezers and medical scissors to directly inoculate the treated small axillary buds by 1 per bottle. On the support pad (axillary bud holder, the same below) equipped with cluster bud differentiation induction liquid medium: 1 / 2MS+1.0mg / L 6-BA+0.4mg / L NAA+0.1mg / LIBA+0.2mg / L GA3+35g / L sucrose, pH 5.8. And cultur...

Embodiment 2

[0061] Select and select the dominant population of Atractylodes japonica, excellent single plant, robust and young Atractylodes japonica seedlings in the sterile culture system as explants, and prune the explants: according to the size of the explants and the number of axillary buds, prune them into 1-2.5 cm long axillary buds with one leaf and one bud, each plant can be trimmed into 3-5 small axillary buds and placed in a petri dish.

[0062] Propagate Atractylodes seedlings as follows:

[0063] 1. Based on the preserved Atractylodes atractylodes tissue culture seedlings as the propagation material, induce the differentiation of cluster buds: Use autoclaved medical tweezers and medical scissors to directly inoculate the treated small axillary buds by 1 per bottle. On the support pad (axillary bud holder, the same below) equipped with cluster bud differentiation induction liquid medium: 1 / 2MS+1.5mg / L 6-BA+0.6mg / L NAA+0.3mg / LIBA+0.4mg / L GA3+45g / L sucrose, pH 5.9. And cultur...

Embodiment 3

[0073] Select and select the dominant population of Atractylodes japonica, excellent single plant, robust and young Atractylodes japonica seedlings in the sterile culture system as explants, and prune the explants: according to the size of the explants and the number of axillary buds, prune them into 1-2.5 cm long axillary buds with one leaf and one bud, each plant can be trimmed into 3-5 small axillary buds and placed in a petri dish.

[0074] Propagate Atractylodes seedlings as follows:

[0075] 1. Based on the preserved Atractylodes atractylodes tissue culture seedlings as the propagation material, induce the differentiation of cluster buds: use autoclaved medical tweezers and medical scissors to directly inoculate the treated small axillary buds into 1 per bottle. On the support pad (axillary bud holder, the same below) equipped with cluster bud differentiation induction liquid medium: 1 / 2MS+2.0mg / L 6-BA+0.8mg / L NAA+0.5mg / LIBA+0.6mg / L GA3+50g / L sucrose, pH 6.0. And cult...

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Abstract

The invention provides a method for propagating Atractylodes Rhizoma seeds and seedlings by utilizing the clustered buds of Atractylodes Rhizoma and application thereof. The method includes the following steps: 1) using the preserved Atractylodes rhizome tissue culture seedlings as the base propagation material, using the Atractylodes Rhizoma tissue culture seedlings The shoot apex and axillary buds were induced to differentiate into clump buds; 2) the clump buds formed by the differentiation induction of clump buds were grown and cultured; 3) the clump buds through the growth culture were subjected to root induction culture; 4) the clump buds were cultured through root induction and culture Carry out strong seedling cultivation; 5) carry out seedling refining treatment (open bottle refining seedlings, matrix refining seedlings) to the clump buds cultivated by the strong seedlings; 6) carry out nursery transplanting and management to the clump buds processed by the cultivating seedlings, and produce Atractylodes Rhizoma 7) Grading and packaging the Atractylodes Rhizoma seeds produced in the nursery.

Description

technical field [0001] The invention relates to the technical field of propagation of medicinal material seedlings, in particular to a method for propagating Atractylodes japonica seeds and seedlings by utilizing clustered buds of Atractylodes japonica and application thereof. Background technique [0002] Atractylodes macrocephala koidz. is a perennial herb of the Compositae family, 50-60cm high, with a massive rhizome. Atractylodes atractylodes is used as medicine with dry rhizomes, warm in nature, sweet and bitter in taste. It has the effects of invigorating the spleen and replenishing qi, diuresis and dampness, and is mainly used for diarrhea, edema, and phlegm-drinking due to spleen deficiency. In this era of rapid development, the market demand for Atractylodes Rhizoma has increased greatly, and it is imminent to find a medicinal material with stable content and good quality. [0003] According to the current situation of cultivation of Chinese herbal medicines in my...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 杨永康李红英覃大吉向级钎陈菲菲殷红清程群问小龙孙举志王晓辉李亚杰田腾飞皮磊
Owner ACAD OF AGRI SCI ENSHI TUJIA MIAOAUTONOMOUS PREFECTURE
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