54 results about "Atractylodes japonica" patented technology

A medical composition for inhibiting the growth of cancer stem cells is disclosed. The medical composition is prepared by mixing herbal medicines with water or alcohol, followed by heating and extraction to obtain a filtrate. One of the medical compositions according to the present invention includes: Coptis chinensis, Scutellaria baicalensis, Phellodendron amourense, Gardenia jasminoides, Radix Glycyrrhizae, and Atractylodes japonica.

The invention discloses a method for preventing and treating bighead atractylodes rhizome root rot and relates to the technical field of traditional Chinese medicinal material disease prevention and treatment. A prevention and treatment agent is prepared from a microbial agent and botanical fungiticide, and July is determined as the optimal bighead atractylodes rhizome white rot prevention and treatment time according to the infection characteristics of bighead atractylodes rhizome root rot pathogenic bacteria and the disease cycle characteristics. According to the method for preventing and treating bighead atractylodes rhizome root rot, all medicine is applied to bighead atractylodes rhizome diseased sites, ventilation and other physical properties of soil can be obviously improved, after the agent acts on diseased roots, further development of the diseased sites is obviously restrained, the fertilizer and water absorption capacity of a root system is improved, new lateral roots are generated, leaves on overground parts are gradually turned light green from yellow, and bighead atractylodes rhizome is restored or basically restored to a normal growth state.

The invention discloses several processing methods for drying fresh bighead atractylodes rhizome, and the bighead atractylodes rhizome is placed in one device or sequentially placed in two different devices for being dried. However, in a re-drying method, the bighead atractylodes rhizome is preliminarily placed in a device in which water on the surfaces of medicinal materials can be easily removed, and then is placed into a device in which water inside the medicinal materials can be easily removed to perform quick drying and high-temperature bacteria densification, so that the effective constituents in the bighead atractylodes rhizome can be prevented from being damaged and the water content of the bighead atractylodes rhizome can quickly reach the standard required by Chinese Pharmacopoeia; moreover, the harm brought about by sulphur fumigation in a processing process is avoided. Meanwhile, through comparing the content and effect of two kinds of effective constituents in the bighead atractylodes rhizome of different processing groups, an optimum processing method is preferably selected, and the method is effectively proved to be accurate and reliable.

The invention provides a propagation method of bighead atractylodes rhizome seedlings. The method comprises the following steps of: (1) selecting bighead atractylodes rhizome explants, and performing bacteria-free treatment on the explants; (2) performing primary culture on the explants; 3 ) carrying out induction culture on callus tissues; (4) performing propagation culture on the callus tissues; (5) performing tufted bud induction culture on the callus tissues; (6) performing rooting culture on tufty buds; and (7) performing acclimatization and transplanting. According to the method disclosed by the invention, typical single plants of dominant types in variety breeding of bighead atractylodes rhizomes can be quickly propagated, the inheritable characters of the bighead atractylodes rhizomes are stabilized in a tissue culture manner, the number of colonies is increased, and preservation, evaluation and utilization of high-quality intermediate materials in the breeding process are facilitated. The invention further provides the application of the method in planting seedlings of the bighead atractylodes rhizomes in greenhouses. According to the application, the survival rate can be maintained, besides, the propagation quantity can be increased, and the propagation area can be arranged as needed.

The invention discloses a pollution-free high-yield planting method of rhizoma atractylodis macrocephalae. The planting method comprises the following steps: (1), selecting well-drained humous soil, building ridged fields for sowing; (2), sowing rhizoma atractylodis macrocephalae during the period from the beginning of Spring to the Qingming festival; (3), when the seedlings grow as tall as 3-6 cm, thinning with the line spacing of 15 cm and the row spacing of 10 cm; (4), when flower buds of rhizoma atractylodis macrocephalae are formed, removing flower buds not for seed reserving in 2-3 batches within 25 days; (5), performing ditch cleaning timely for drainage due to the fact that rhizoma atractylodis macrocephalae is easily influenced by waterlogging during growth and intolerant to drought, so as to realize that the fields are free of stagnant water when rain stops; (6), performing pest control; (7), harvesting in good time: harvesting after the first-frost date, wherein when harvesting is performed, the stem leaves of rhizoma atractylodis macrocephalae are withered, the upper leaves harden and are friable, and the lower leaves are yellowish.

The invention relates to a method for interplanting corns in bighead atractylodes rhizomes in a semi-humid and semiarid northern area. The method comprises the production steps of: (1) variety selection; (2) seed treatment; (3) land preparation and fertilizing, to be specific, carrying out land preparation, applying 3000kg of farm manure to per mu of the land (mu is a unit of area, which is identical to 0.0667 hectares), together with 50kg of calcium phosphate which is taken as a base fertilizer, deeply ploughing by 20cm to make furrows which are 1-1.5m wide, planting 4-6 rows of bighead atractylodes rhizomes in each of the furrows, planting 2 rows of corns by a spacing of 60cm, and sequentially interplanting; (4) sowing; (5) field management; and (6) harvesting. According to the method for interplanting corns in bighead atractylodes rhizomes in the semi-humid and semiarid northern area, by the demands of the bighead atractylodes rhizomes and the corns on the light, heat and water as well as complementarity among the crops, the traditional agricultural production mode is changed, and therefore, the unit yield is improved and the yield and efficiency are increased.

The present invention relates to a preparation technology and a production method for an integrated new formulation of a bighead atractylodes rhizome and monkshood decoction, wherein the raw materials of active ingredients comprise: 6 parts by weight of bighead atractylodes rhizome, 10 parts by weight of monkshood, 3 parts by weight of licorice, 4.5 parts by weight of ginger, and 6 parts by weight of Chinese-date. The production process of the integrated new formulation comprises the following steps of: supersonic air-jet crushing, alcohol-water extraction, ultrasonic crushing extraction, water decocting concentration, supersonic spray drying, nanometer grinding, high pressure homogenization, nano particle preparation and the like. According to the invention, the advantages, such as multi-synergism, multi-target, embodied by a nano-carrier coupling technique, are focused to be taken of. After the integrated new formulation of bighead atractylodes rhizome and monkshood decoction is subjected to scale production, the production cost can be greatly reduced, the quality of products is greatly improved and the targeting property, and the sustained and controlled release performance of the medicament are enhanced. The internal and external administration of the integrated new formulation of bighead atractylodes rhizome and monkshood decoction can be realized simultaneously, and the medicament can be separately taken in four time slices according to the midnight-noon ebb-flow and qi and the blood flowing rule of a human body. The integrated new formulation of bighead atractylodes rhizome-monkshood decoction can be further prepared into films and transdermal agents, which are respectively stuck to different parts of a human body according to the midnight-noon ebb-flow and qi and blood flowing rule of the human body, and can be directly absorbed through skin.

The invention relates to an antibacterial Bidens pilosa liquid soap and a production method thereof. The antibacterial Bidens pilosa liquid soap is characterized in that the liquid soap comprises, by weight, 2-6 parts of Bidens pilosa extract, 1-3 parts of Atractylodes japonica extract, 5-10 parts of an anionic surfactant, 0.01-0.1 parts of natural essence, 0.1-2.0 parts of sodium chloride, 1-5 parts of a humectant, 1-5 parts of a chelating agent and 10-40 parts of water; and the anionic surfactant is one or more of polyoxyethylene fatty alcohol ether sodium sulfate, disodium polyoxyethylene ether sulfosuccinate and sodium dodecyl benzene sulfonate. The antibacterial Bidens pilosa liquid soap is safe, has no irritation, can remove and inhibit various growing bacteria, and effectively removes various oil stains and spots; and the humectant in the liquid soap is helpful for moisturizing skins and softening stratum corneum, and promotes metabolism of skins in order to realize a skin moistening effect.

The invention comprises a series of preparing steps as following: taking powder of white atractylodes rhizome, adding water, distilling by using ultrasonic, filtering, adding 95%alcohol to filtrate and standing overnight, retrieving alcohol from filtrate, distilling sunk fluid of alcohol by ethyl acetate, drying out ethyl acetate, fetching constant volume of the treated fluid as solution of sample; dropping a drop of solution of sample on G thin layer plate with silica gel, 20 minute saturation in advance in developing agent, then developing, fetching out, air drying, spraying alcohol solution of 10% sulfuric acid, heating till obtaining clear color speck; scanning the speck by using fluorescence in 330nm wavelength obtains fingerprint needed. Common characteristics of TLC fingerprints from 11 batches of white atractylodes rhizome are as standard fingerprint. The invention provides method for controlling quality and discriminating true and false of medicinal materials.

The invention discloses a resuscitation method of CAR-T cells. The resuscitation method comprises the following steps: taking out a cryopreservation bag loaded with CAR-T cells and cryopreservation liquid from liquid nitrogen, adding cryopreservation liquid preheated at 36-40 DEG C, shaking the cryopreservation bag for thawing under the condition of 36-40 DEG C water bath for 1-2 minutes, performing centrifuging, and discarding supernate; and adding resuscitation liquid preheated at 36-40 DEG C, performing mixing, performing centrifuging, discarding supernate, and resuspending the cells by using a cell culture medium, wherein the resuscitation liquid comprises the following components of 10-50 [mu] g/L L-proline, 10-100 mg/L sodium selenite, 2.0-2.2 mg/L sodium pyruvate, 0.2-0.5 mg/L f NaOH, 0.2-0.4 mg/L magnesium chloride, 15-25 mg/L astragaloside, 10-50 mg/L atractylodes macrocephala lactone II, 15-30 mg/L sodium diatrizoate and the balance the cell culture medium. By adopting the resuscitation method disclosed by the invention, the motility rate, the multiplication capacity and tumor cell removal capacity of cells can be well maintained. And the method is simple and convenient to operate, low in cost and beneficial to industrial use.

The invention belongs to the technical field of medicine, and discloses a quality control method for detecting content of butenolide I, butenolide II and 23-acetyl alisol B in a rhizoma alismatis-largehead atractylodes rhizome tablet. The content of one or more components is determined simultaneously or separately by high performance liquid chromatography. C8 and C18 are used as fillers. Isocraticelution or gradient elution is carried out by using 0-100% acetonitrile or methanol as a mobile phase A and 100-0% ultrapure water or glacial acetic acid or a phosphoric acid solution as a mobile phase B. A flow rate is 0.1-2 ml/min, detection wavelength is 203-320 nm, and column temperature is 20-40 DEG C. The method of the invention controls the quality of active components of two medicinal materials in the prescription of the rhizoma alismatis-largehead atractylodes rhizome tablet, and so the quality control of the preparation is more comprehensive, accurate and effective, thereby helpingto ensure the medicine safety and validity. Moreover, the determination method has the advantages of simple operation, accurate result and good precision, stability and reproducibility, is an advancedquality control method and facilitates the standardized production of the medicine.

The invention discloses a method for domesticizing wild atractylodes japonica in Changbai Mountains. The method sequentially includes following steps: using rhizomes for asexual reproduction: digging out rhizomes of the wild atractylodes japonica, planting the rhizomes in an experimental field for asexual reproduction to enable the wild atractylodes japonica to adapt to ecological environment of domestic planting; and using seeds for sexual reproduction: sowing quality seeds obtained by directional strain selection for sexual reproduction in the next year; using rhizomes for asexual reproduction; and using the rhizomes of the previous year for cultivation for asexual reproduction in the third year; and allowing domestication characters to stabilize in the fourth year. By the method, the wild atractylodes japonica is domesticized into a novel variety suitable for garden planting and pastoral ecological environments, and high quality and high yield are realized.

Plants that are substantially utilized as the medicinal ingredients in a selected single prescription of the traditional Oriental and Korean medicine are planted together in close proximity to each other. The plants provide therapeutic effects to the body of a person breathing the fragrance emitted by the plants and observing the plants. The plants are selected in accordance with the prescription of Sagunja-tang (a ginseng; a Atractylodes japonica koidz; a Glycyrrhiza uralensis Fisch; and a Pinus densiflora) or Samul-tang (an Angelica gigas Nakia; a Cnidium oficinale; a Paeonia lactiflora Pall; and a Rhemanniae glutinosa Libosch) or Palmul-tang (the eight plants selected according to Sagunja-tang and Samul-tang) or Shipjundaebo-tang (the eight plants according to Palmul-tang plus an Astrualus membranaceus Bunge var and a Cinnamon). These plants can be planted in a portable medium including at least a flowerpot.

The invention provides an energy-saving and environment-friendly bird repelling device for atractylodes macrocephala planting. The device comprises a mounting frame, wherein a wind power mechanism is mounted at the top of the mounting frame, a driving assembly matching the wind power mechanism is mounted inside the mounting frame, the driving assembly comprises a vertical shaft, a conical disc and a rotating ring, a transverse shaft is mounted inside the mounting frame and below the conical disc, reflector sets are installed at the end of the transverse shaft, a noise assembly is connected to the bottom of the rotating ring, and a detection assembly is installed on the transverse shaft. The rotating shaft drives the vertical shaft and the rotating ring to rotate in a differential mode through a speed regulating gear set, the conical disc drives a conical sleeve to rotate and deflect up and down, the reflector sets at the two ends of the transverse shaft swing up and down along with rotation of the transverse shaft, then light reflection areas of the reflector sets are enlarged, the reflector sets can horizontally rotate along with a support base, the light bird repelling effect is improved, the transverse shaft moves a pendulum back and forth and hits a noise plate to make noise, a sound bird repelling function is achieved, and the device is simple in structure, low in use cost, capable of saving energy, environmentally friendly and convenient to use.

The invention discloses a radix aucklandiae, villous amomum, citrus aurantium, and atractylodes rhizome capsule, which is prepared through the following steps by using radix aucklandiae, bran-fried citrus aurantium, villous amomum, and bran-fried atractylodes rhizome as raw drugs: properly extracting the raw drugs, adding auxiliary materials, stirring uniformly, and filling into a capsule shell. The drug has the effects of invigorating spleen and appetizing, moving qi and dispersing glomus; the effective components of the drug has a ralatively high concentration, so the effect is faster and the dose is small; and the above charateristics is favorable to the quality control of the preparation and the improvement of drug quality.

The invention provides a method for identifying atractylodes macrocephala koidz in a donkey-hide glue blood-enriching preparation by using a thin layer chromatography method, wherein the thin layer chromatography method comprises: sucking 5-20 [mu]l of a test product solution and 5-20 [mu]l of a reference product solution, respectively loading onto a thin layer chromatography plate, developing by adopting a n-hexane-ethyl acetate-formic acid mixed solution as a developing agent, taking out, carrying out air drying, spraying a coloring agent, placing at a 105 DEG C place, baking until the spot color is clear, placing under a 365 nm ultraviolet lamp, carrying out visual inspection, and in a test product chromatogram, displaying the fluorescent spots having the same color at the positions corresponding to the reference product chromatogram and the reference herb chromatogram. According to the present invention, the method has advantages of simple operation, rapid separation, accurate separation effect, good reproducibility and strong specificity, and provides the basis for the further quality control of the donkey-hide glue blood-enriching preparation.

The invention relates to a preparation technology and a production method of an integrative new dosage form of a Rhizoma Atractylodis Macrocephalae and Radix aconiti lateralis preparata decoction. The raw materials for the effective components of the decoction comprise 2 parts by weight of licorice, 4 parts by weight of Rhizoma Atractylodis Macrocephalae and 1.5 parts by weight of Radix aconiti lateralis preparata. The production method comprises the production processes of ultrasonic airflow crushing, alcohol-water extraction, supersonic wave crushing extraction, water decoction and concentration, ultrasonic spray drying, nanometer grinding, high-pressure homogenization, nanoparticle preparation and the like. The advantages comprising multiple cooperation, multiple targeting and the like of a nanometer carrier combination technology are utilized in the invention. Great production cost reduction, great product quality improvement, medicine targeting strengthening, and medicine sustained and controlled release property strengthening are realized after the large-scale production. Internal and external administration can be simultaneously carried out, and the decoction can be respectively administrated in four time periods according to the midnight-noon ebb-flow and human qi and blood flow rules. The decoction can also be processed to prepare a film agent and a transdermal agent which are respectively plastered on different positions of a human body according to the midnight-noon ebb-flow and human qi and blood flow rules, and the effective components can be directly absorbed through skins.

The invention discloses a bud inhibitor of large-headed atractylodes. The inhibitor is prepared by maleic hydrazide, nonionic emulsifier, diethanolamine and gold V peptides in a weight ratio. The inhibitor enters the plants by corneous layers of leaves and can greatly inhibit the growth of buds so as to control the growth, blooming and seeding of buds; in addition, the inhibitor can effectively destroy the chain layer of plants and cut the connecting layer between the upper part and the lower part, so that the nutrients are kept and moved to the root to expand the root, and the aims of improving yield and increasing profit are achieved.

The present invention relates to a composition for prevention and treatment of osteoporosis comprising the extract of medicinal herb mixture. The composition includes an extract of a medicinal herb mixture of Angelica gigas, Paeonia japonica, Atractylodes japonica, Wolfiporia extensa, and Platycodon grandiflorum, and a composition formed by more than one of Gardenia jasminoides, Glycyrrhiza uralensis, Zingiber officinale and Mentha arvensis var. piperascens. The composition is safe in a body, can increase the weight and the length of bones, can increase the bone mineral density, especially, when the optimal content includes the composition of Platycodon grandiflorum, can significantly increase alkaline acid phosphatase of osteoblast, and can significantly inhibit tartrate-resistant acid phosphatase of osteoclast, so as to effectively realize improvement of therapeutic agents for multiple bone diseases, including osteoporosis.

The invention discloses an external composition for resisting skin aging and application thereof, and belongs to the technical field of daily cosmetics. The external composition is formed by compounding a bletilla striata polysaccharide extract, an atractylodes macrocephala polysaccharide extract, an ampelopsis japonica polyphenol extract and a lotus flavone extract according to the mass ratio of (1-4): (1-6): (1-3): (1-3), the composition is prepared into emulsion to be used for human skin, a stronger skin aging resisting effect is shown, the four extracts have a synergistic interaction effect, and the anti-skin aging effect of the composition is obviously improved.

The invention discloses a nourishing compound Atractylodes macrocephala Koidz ointment, comprising 250-350 parts of Atractylodes macrocephala Koidz, 25-35 parts of Rosa Laevigata, 30-50 parts of Elaeagnus angustifolia, 30-50 parts of dates, 10-15 parts of herba cistanches, 5-10 parts of american ginseng, 20-30 parts of spines plus and 30-50 parts of hawthorn. The invention has the effect of keeping simple Baizhu ointment on disharmony between spleen and stomach, tasteless diet, diarrhea of stool, etc., and Atractylodes macrocephala Koidz ointment has that effect of resisting age, resisting fatigue and regulating immunity, and can increase vitamin C ingredient, minerals and trace elements necessary for human body, thereby promoting absorption of human body and maintaining human body health.

The invention discloses a novel terpenoid obtained from Atractylodes japonica, a preparation method of the novel terpenoid and application of the novel terpenoid in the aspect of gastric cancer prevention. The molecular formula of the novel terpenoid is C30H44O4, and the molecular weight of the novel terpenoid is 468. The novel terpenoid is obtained by cold leaching and extraction with ethanol and separation and purification with silica gel column chromatography, gel column chromatography, semi-preparative high-performance liquid chromatography and other methods, and the chemical structure of the novel terpenoid is confirmed and illuminated through one-dimensional and two-dimensional nuclear magnetic resonance technologies and mass spectrum means. A CKK-8 experiment result shows that the novel terpenoid has an inhibition effect on human gastric cancer MGC-803 cells. The novel terpenoid separated and prepared from Atractylodes japonica is novel in structure, can be used as a lead compound for developing novel antitumor drugs, and is expected to be further used as a clinical drug for treating cancers.