The invention provides a method for identifying atractylodes macrocephala koidz in a donkey-hide glue blood-enriching preparation by using a thin layer chromatography method, wherein the thin layer chromatography method comprises: sucking 5-20 [mu]l of a test product solution and 5-20 [mu]l of a reference product solution, respectively loading onto a thin layer chromatography plate, developing by adopting a n-hexane-ethyl acetate-formic acid mixed solution as a developing agent, taking out, carrying out air drying, spraying a coloring agent, placing at a 105 DEG C place, baking until the spot color is clear, placing under a 365 nm ultraviolet lamp, carrying out visual inspection, and in a test product chromatogram, displaying the fluorescent spots having the same color at the positions corresponding to the reference product chromatogram and the reference herb chromatogram. According to the present invention, the method has advantages of simple operation, rapid separation, accurate separation effect, good reproducibility and strong specificity, and provides the basis for the further quality control of the donkey-hide glue blood-enriching preparation.