Method for identifying atractylodes macrocephala koidz in donkey-hide glue blood-enriching preparation

A technique for donkey glue and preparation, which is applied in the field of identification of Atractylodes atractylodes in the identification of donkey glue blood-enriching preparations, can solve the problems of poor quality control, instability of atractylodes ketone, identification of Atractylodes atractylodes, etc., and achieves simple operation, strong specificity, and rapid separation effect. Effect

Active Publication Date: 2017-02-01
JIUZHITANG
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0003] In the 2010 edition of "Chinese Pharmacopoeia", under the identification item of Lvjiao Buxue Granules, angelica and astragalus were qualitatively identified by TLC, but Atractylodes macrocephala was not identified, so its quality could not be well controlled
In addition, atractylodes ketone is very unstable, and it will transform into Atractylodes lactone III when exposed to heat and light (Ye Yan, Cheng Xuemei, Wu Guixin. Thin-layer chromatography research on Atractylodes macrocephala [J]. Shizhen Guoyi Guoyao, 2009, 20( 7): 1702-1703.)
Therefore, it is inappropriate to use Atractylodes atractylodes to identify Atractylodes macrocephala in donkey glue blood-enriching preparations and even related traditional Chinese medicine compound preparations.

Method used

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  • Method for identifying atractylodes macrocephala koidz in donkey-hide glue blood-enriching preparation
  • Method for identifying atractylodes macrocephala koidz in donkey-hide glue blood-enriching preparation
  • Method for identifying atractylodes macrocephala koidz in donkey-hide glue blood-enriching preparation

Examples

Experimental program
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Effect test

Embodiment 1

[0041] A method for identifying Atractylodes Rhizoma Atractylodes Rhizome in donkey glue blood-tonifying preparation, comprising the following steps:

[0042] (1) Preparation of the test solution:

[0043] Take 20g of this product, grind it well, add 50ml of ether, heat and reflux for 1 hour, take it out, let it cool, filter, evaporate the filtrate to dryness, add 1ml of ethyl acetate to the residue to dissolve, and use it as the test solution;

[0044] (2) Preparation of contrast medicinal material solution:

[0045] Get Atractylodes macrocephala contrast medicinal material 0.5g, make contrast medicinal material solution with the method of step (1);

[0046] (3) Preparation of reference substance solution:

[0047] Take 0.5 mg of Atractylodes lactone III, weigh it accurately, add 10 ml of methanol to dissolve, and make a reference solution containing 0.05 mg of Atractylodes lactone III per 1 ml;

[0048] (4) TLC identification:

[0049] Take 5 μl each of the test solution...

Embodiment 2

[0051] A method for identifying Atractylodes Rhizoma Atractylodes Rhizome in donkey glue blood-tonifying preparation, comprising the following steps:

[0052] (1) Preparation of the test solution:

[0053] Take 15g of this product, grind it well, add 50ml of ether, heat and reflux for 2 hours, take it out, let it cool, filter, evaporate the filtrate to dryness, add 1ml of ethyl acetate to the residue to dissolve, and use it as the test solution;

[0054] (2) Preparation of contrast medicinal material solution:

[0055] Get Atractylodes macrocephala contrast medicinal material 0.5g, make contrast medicinal material solution with the method of step (1);

[0056] (3) Preparation of reference substance solution:

[0057] Take 0.5 mg of Atractylodes lactone III, weigh it accurately, add 10 ml of methanol to dissolve, and make a reference solution containing 0.05 mg of Atractylodes lactone III per 1 ml;

[0058] (4) TLC identification:

[0059] Take 5 μl each of the test solutio...

Embodiment 3

[0061] A method for identifying Atractylodes Rhizoma Atractylodes Rhizome in donkey glue blood-tonifying preparation, comprising the following steps:

[0062] (1) Preparation of the test solution:

[0063] Take 25g of this product, grind it evenly, add 50ml of ether, heat and reflux for 1.5 hours, take it out, let it cool, filter, evaporate the filtrate to dryness, add 2ml of ethyl acetate to the residue to dissolve, and use it as the test solution;

[0064] (2) Preparation of contrast medicinal material solution:

[0065] Get Atractylodes macrocephala contrast medicinal material 0.5g, make contrast medicinal material solution with the method of step (1);

[0066] (3) Preparation of reference substance solution:

[0067] Take 0.5 mg of Atractylodes lactone III, weigh it accurately, add 10 ml of methanol to dissolve, and make a reference solution containing 0.05 mg of Atractylodes lactone III per 1 ml;

[0068] (4) TLC identification:

[0069] Take 5 μl each of the test sol...

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Abstract

The invention provides a method for identifying atractylodes macrocephala koidz in a donkey-hide glue blood-enriching preparation by using a thin layer chromatography method, wherein the thin layer chromatography method comprises: sucking 5-20 [mu]l of a test product solution and 5-20 [mu]l of a reference product solution, respectively loading onto a thin layer chromatography plate, developing by adopting a n-hexane-ethyl acetate-formic acid mixed solution as a developing agent, taking out, carrying out air drying, spraying a coloring agent, placing at a 105 DEG C place, baking until the spot color is clear, placing under a 365 nm ultraviolet lamp, carrying out visual inspection, and in a test product chromatogram, displaying the fluorescent spots having the same color at the positions corresponding to the reference product chromatogram and the reference herb chromatogram. According to the present invention, the method has advantages of simple operation, rapid separation, accurate separation effect, good reproducibility and strong specificity, and provides the basis for the further quality control of the donkey-hide glue blood-enriching preparation.

Description

technical field [0001] The invention belongs to the technical field of detection of traditional Chinese medicine preparations, and in particular relates to a method for identifying Atractylodes macrocephala in donkey glue blood-tonifying preparations. Background technique [0002] Atractylodes macrocephala has the effects of invigorating the spleen and replenishing qi, drying dampness and promoting diuresis, antiperspirant, and preventing miscarriage. It is used for insufficiency of the spleen, lack of food, abdominal distension and diarrhea, phlegm and retention, dizziness, edema, spontaneous sweating, fetal restlessness, etc. It is an important medicine for invigorating the spleen in traditional Chinese medicine. . The main components of Atractylodes macrocephala are atractylodes ketone and atractylodes lactone, etc. Modern pharmacological studies have proved that atractylodes ketone and lactones are the effective components of Atractylodes macrocephala for anti-inflammato...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90
Inventor 叶惠煊谭真鲁鲁黄胜陈四娟谢安谷陟欣
Owner JIUZHITANG
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