A cryopreservation device for human ovarian tissue under liquid nitrogen

Abstract

A device for freeze preservation of a human ovarian tissue under liquid nitrogen comprises a pipe body; one end of the pipe body is provided with an opening, the opening is equipped with a sealing cover, the sealing cover and the pipe body can be detachably connected, and a sealed storage space is enclosed by the sealing cover and the pipe body; the inner top surface of the sealing cover is provided with a forking device for forking the human ovarian tissue. The forking device comprises a plurality of fine needles which are arranged in parallel, and fixed ends of the fine needles are vertically fixed on the inner end surface of the sealing cover, free ends of the fine needles extend to the pipe body along the axial direction of the pipe body. The forking device comprises 3 fine needles, the 3 fixed ends of the 3 fine needles are in head-to-tail connection to form a regular triangle, and the circle center of the inner end face of the sealing cover is the center of the regular triangle. The device is simple in structure and easy to use; the forking device can avoid physical damage of blind clipping with forceps under liquid nitrogen on an ovarian follicle enriched cortical part in the ovarian tissue, and also avoids cross contamination with other iatrogenic substances in a liquid nitrogen tank.

Description

Technical field [0001] The invention relates to the technical field of preservation of human fertility, in particular to a cryopreservation device for human ovarian tissue under liquid nitrogen. Background technique [0002] High-dose systemic chemotherapy or radiotherapy in the process of cancer cure will seriously affect the fertility of young women. Statistics show that young patients with malignant tumors will be infertile after receiving radiotherapy or chemotherapy. At present, there are three main methods to protect the fertility of cancer patients: embryo freezing, oocyte freezing and ovarian tissue freezing. But embryo freezing is not suitable for pre-pubertal women or women who have no spouse or sperm; oocyte freezing requires a longer ovulation induction cycle, and there is a risk of delaying the best time to treat tumors; ovarian tissue freezing can store a large number of follicles at the same time. And the selection of materials is not limited by time, and it has b...

AI Technical Summary

Problems solved by technology

The cryopreservation tube method is actually an indirect liquid nitrogen contact method because the ovarian tissue is first sealed in the tube during the operation, and then put into liquid nitrogen. The cooling rate is much lower than the direct contact method, resulting in the number of follicles at all levels after recovery and proliferative activity were significantly lower than those of other methods

The droplet vitrification method and the solid surface vitrification method are both direct liquid nitrogen contact methods. Although they can both achieve a good cooling rate, they need to use tweezers to put ovarian tissue into a cryovial under liquid nitrogen after putting in liquid nitrogen. In the process, due to the volatility of liquid nitrogen, the tube loading operation interferes a lot, and the gripping force of tweezers under liquid nitrogen is not easy to control, and it is very easy to cause physical extrusion damage to the sensitive cortical follicle area

The metal screen method is to place the ovarian tissue on the metal grid first, put it into liquid nitrogen and then put it into the frozen storage bag. Although the direct contact between the tweezers and the ovarian tissue is avoided, the frozen storage bag can be stored at -196°C. The ability to resist mechanical pulling is not as good as cryopreservation tubes, which are easy to crack, and the shape of the freezer storage bag is not fixed, so it is not easy to freeze in an orderly manner

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