Propagation method of bighead atractylodes rhizome seedlings and application thereof

A technology of Atractylodes macrocephala and seedlings, applied in the field of plants, can solve the problems of prolonging the breeding period of varieties, increasing the difficulty of variety breeding, and being unfavorable for homozygous traits of offspring plants, and achieving the effect of expanding the number of populations

Inactive Publication Date: 2016-01-13
ACAD OF AGRI SCI ENSHI TUJIA MIAOAUTONOMOUS PREFECTURE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Atractylodes Rhizoma Atractylodes Rhizoma Atractylodes Rhizoma Atractylodes Rhizoma Atractylodes Rhizoma Atractylodes Rhizome belongs to cross-pollination crops, self-pollination is not strong or seeds are shriveled, and cross-pollination se

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Select the dominant type, excellent single plant, strong and tender Atractylodes atractylodes axillary buds as explants, and carry out aseptic treatment to it: take the young buds on the stem, trim the excess leaves, remove the dust particles on the surface with a soft brush; tap water Rinse well (60 minutes), blot the moisture on the bud surface, soak for 15 seconds with 75% alcohol on the sterilized ultra-clean workbench, rinse with sterile water for 3 times, then replace with 0.01% mercuric chloride solution Soak for 30 seconds, rinse with sterile water for 3 times, and finally soak with 1% sodium hypochlorite solution for 5 minutes, stir continuously during this period, rinse with sterile water for 4 times, pour out the excess sterile water, and place the axillary buds in a petri dish for later use.

[0055] Propagate Atractylodes seedlings as follows:

[0056] 1. Primary culture of Atractylodes macrocephala seedlings: Use autoclaved medical tweezers and medical sci...

Embodiment 2

[0063] Select the dominant type, excellent single plant, strong and tender Atractylodes atractylodes axillary buds as explants, and carry out aseptic treatment to it: take the young buds on the stem, trim the excess leaves, remove the dust particles on the surface with a soft brush; tap water Rinse well (60 minutes), blot the moisture on the bud surface, soak 45 seconds with 75% alcohol on the sterilized ultra-clean workbench, rinse 4 times with sterile water, and then use 0.2% mercuric chloride solution Soak for 120 seconds, rinse with sterile water for 4 times, and finally soak with 10% sodium hypochlorite solution for 15 minutes, stir continuously during this period, rinse with sterile water for 5 times, pour out the excess sterile water, and place the axillary buds in a petri dish for later use.

[0064] Propagate Atractylodes seedlings as follows:

[0065] 1. Primary culture of Atractylodes macrocephala seedlings: Use autoclaved medical tweezers and medical scissors to pl...

Embodiment 3

[0072] Select the dominant type, excellent single plant, strong and tender Atractylodes atractylodes axillary buds as explants, and carry out aseptic treatment to it: take the young buds on the stem, trim the excess leaves, remove the dust particles on the surface with a soft brush; tap water Rinse well (60 minutes), blot the moisture on the bud surface, soak 30 seconds with 75% alcohol on the sterilized ultra-clean workbench, rinse 3 times with sterile water, and then use 0.1% mercuric chloride solution Soak for 60 seconds, rinse with sterile water for 3 times, and finally soak with 5% sodium hypochlorite solution for 10 minutes, stir continuously during this period, rinse with sterile water for 4 times, pour out the excess sterile water, and place the axillary buds in a petri dish for later use.

[0073] Propagate Atractylodes seedlings as follows:

[0074]1. Primary culture of Atractylodes macrocephala seedlings: Use autoclaved medical tweezers and medical scissors to place...

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Abstract

The invention provides a propagation method of bighead atractylodes rhizome seedlings. The method comprises the following steps of: (1) selecting bighead atractylodes rhizome explants, and performing bacteria-free treatment on the explants; (2) performing primary culture on the explants; 3 ) carrying out induction culture on callus tissues; (4) performing propagation culture on the callus tissues; (5) performing tufted bud induction culture on the callus tissues; (6) performing rooting culture on tufty buds; and (7) performing acclimatization and transplanting. According to the method disclosed by the invention, typical single plants of dominant types in variety breeding of bighead atractylodes rhizomes can be quickly propagated, the inheritable characters of the bighead atractylodes rhizomes are stabilized in a tissue culture manner, the number of colonies is increased, and preservation, evaluation and utilization of high-quality intermediate materials in the breeding process are facilitated. The invention further provides the application of the method in planting seedlings of the bighead atractylodes rhizomes in greenhouses. According to the application, the survival rate can be maintained, besides, the propagation quantity can be increased, and the propagation area can be arranged as needed.

Description

technical field [0001] The invention relates to the field of plants, in particular to a method for propagating Atractylodes macrocephala seedlings. Background technique [0002] Atractylodes macrocephala Koidz (AtractylodesmacrocephalaKoidz.) is a perennial herb of Compositae, also known as Yushu, Zhezhu, Dongzhu and so on. As a traditional Chinese medicinal plant, it has the functions of invigorating the spleen and replenishing qi, drying dampness and diuresis, antiperspirant and preventing miscarriage. Studies in recent years have shown that Atractylodes macrocephala has diuretic, anti-tumor, anti-bacterial, anti-inflammatory, anti-diabetic, and anti-aging effects. Due to the wide range of uses of Atractylodes Rhizoma Atractylodes Rhizoma Atractylodes Rhizoma Atractylodes Rhizoma Atractylodes Rhizoma Atractylodes Rhizoma Atractylodes Rhizome is widely used and has a large market demand, it can be popularized and planted all over the country. [0003] At present, in the p...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G9/00
Inventor 李红英覃大吉王应玲向极钎杨永康陈菲菲殷红清程群马进叶紫云
Owner ACAD OF AGRI SCI ENSHI TUJIA MIAOAUTONOMOUS PREFECTURE
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