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Detection method for atractylodes macrocephala koidz medicinal materials

A detection method and technology for medicinal materials are applied in the detection field of Atractylodes Rhizoma medicinal materials to achieve the effects of good separation of characteristic peaks, high similarity, and medication safety assurance.

Inactive Publication Date: 2014-02-26
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no report of decocting Atractylodes macrocephala for HPLC fingerprint analysis

Method used

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  • Detection method for atractylodes macrocephala koidz medicinal materials
  • Detection method for atractylodes macrocephala koidz medicinal materials
  • Detection method for atractylodes macrocephala koidz medicinal materials

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1 The detection method of Atractylodes macrocephala medicinal material of the present invention

[0035] (1) Accurately weigh about 2g of Atractylodes macrocephala, add 40ml of water, decoct twice in a small beaker for 1 hour each time, filter and concentrate to about 10ml, transfer to an evaporating dish, evaporate to dryness, add 20ml of methanol, sonicate for 20min, filter , concentrated, and fixed volume to 5ml, as the test solution.

[0036] (2) Accurately weigh about 3mg of Atractylodes lactone III, and make it into a methanol solution of about 10ug / ml as a reference solution.

[0037] (3) Precisely draw the reference solution and the test solution into the Shimadzu LC-20AT liquid chromatograph, elute with acetonitrile-water system as the mobile phase, and detect under ultraviolet light. The sample volume is 20 μl, and the liquid phase conditions are as follows:

[0038] Chromatographic column: Diamonsil diamond C18, 250mm × 4.6mm × 5μm; detection wav...

Embodiment 2

[0041] The investigation of embodiment 2 detection method of the present invention

[0042] 1 Instruments and materials

[0043] 1.1 Medicinal materials

[0044] Table 1 Sources of Atractylodes macrocephala

[0045]

[0046] 1.2 Instruments

[0047] High performance liquid chromatography (Shimadzu LC-20AT), FA2004N electronic balance (Shanghai Huyueming Scientific Instrument Co., Ltd.), BUG25-12 ultrasonic cleaner (Branson Ultrasonic (Shanghai) Co., Ltd.), Diamonsil diamond C18 (250mm ×4.6mm×5μm) chromatographic column.

[0048] 1.3 Reagent

[0049] Atractylodes lactone III (standard product); Methanol and acetonitrile used are chromatographic alcohols, and water is double distilled water.

[0050] 2. Optimization of chromatographic conditions

[0051] 2.1 Selection of mobile phase system

[0052] Diamonsil diamond C18 (250mm×4.6mm×5μm) chromatographic column, DAD detector, compare the following mobile phase systems: (1) methanol (A): water (B) (2) methanol / acetoni...

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Abstract

The invention discloses a detection method for atractylodes macrocephala koidz medicinal materials. The method adopts HPLC (high performance liquid chromatography) fingerprint spectrum for detection and includes the operation steps of: (1) preparing sample solution; (2) preparing reference solution; and (3) respectively and precisely absorbing the sample solution and the reference solution to inject into a liquid chromatograph so as to elute by taking an acetonitrile-water system as a flowing phase and detect at wavelengths of 248+ / -5nm. After the atractylodes macrocephala koidz medicinal materials are decocted with water, and then the HPLC fingerprint spectrum is applied for detection, so that quality of the atractylodes macrocephala koidz medicinal materials can be reflected more truly. Moreover, chromatographic conditions such as the flowing phase are selected specifically, so that chromatogram baselines are stable and convenient to integrate, resolution of characteristic peaks is good, and similarity among different medicinal materials is high. The detection method for the atractylodes macrocephala koidz medicinal materials can be effectively used for quality control of atractylodes macrocephala koidz and provides a guarantee of medication security of the atractylodes macrocephala koidz medicinal materials.

Description

technical field [0001] The invention relates to a detection method of Atractylodes macrocephala medicinal material, in particular, it is a detection method using HPLC fingerprint spectrum. Background technique [0002] Atractylodes macrocephala is the dry rhizome of Compositae (Atractylodes macrocephala Koidz.), which tastes bitter, sweet, and warm in nature. Atractylodes macrocephala is a commonly used medicinal material in traditional Chinese medicine. However, the current pharmacopoeia for the identification of Atractylodes macrocephala only involves simple identification methods such as traits and thin layers, and cannot provide an intuitive method for identifying the authenticity of Atractylodes macrocephala. [0003] At present, high performance liquid chromatography technology is a commonly used quantitative and qualitative detection method in the quality inspection of traditional Chinese medicine, which can intuitively analyze the authenticity of medicinal materials....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N30/12
Inventor 彭腾李鸿翔邓赟贺钢民袁海梅杨菁黎豫川
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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