Composition for prevention and treatment of osteoporosis comprising the extract of medicinal herb mixture

A composition and bone disease technology, which is applied in the direction of drug combination, food ingredients containing natural extracts, medical preparations containing active ingredients, etc., can solve the problems that the treatment effect of osteoporosis cannot be displayed, and achieve weight and length increase , increase bone density, increase the effect of alkaline phosphatase activity

Active Publication Date: 2018-08-28
金秀昡
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the mixed composition disclosed in the above-mentioned documents cannot exhibit a significant therapeutic effect on osteoporosis. Therefore...

Method used

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  • Composition for prevention and treatment of osteoporosis comprising the extract of medicinal herb mixture
  • Composition for prevention and treatment of osteoporosis comprising the extract of medicinal herb mixture
  • Composition for prevention and treatment of osteoporosis comprising the extract of medicinal herb mixture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Embodiment 1. Preparation of mixed crude medicinal material extract

[0057] 1-1. Raw medicinal material screening and pretreatment process

[0058] For 150g of Chinese Angelica, 150g of Radix Paeoniae Alba, 150g of Atractylodes Rhizome, 150g of Poria, 100g of Gardenia, 100g of Licorice, 100g of Bellflower, 50g of Dried Ginger and 50g of Peppermint, after removing foreign matter, finely cut them into about 5cm. Extracts easily and rinses cleanly with water. The purpose of finely chopping raw herbs is to facilitate their handling and extraction.

[0059] 1-2. Preparation of hot water extract of mixed raw medicinal materials

[0060] The material obtained in the above-mentioned process is put into the extraction device, and extracted with an extraction solvent. As an extraction solvent, pure water was added 5 times as much as the crude drug, and extracted at a temperature of 95° C. for 6 hours with a cooling extraction device. The extract was soaked naturally for 1 to...

Embodiment 2

[0072] Example 2. Activity evaluation of osteoblasts

[0073] 2-1. Cell culture and differentiation induction

[0074] MC3T3-E1 osteoblast cells (MC3T3-E1 osteoblastic cells) derived from raw mice were purchased from American Type Culture Collection (ATCC, American Type Culture Collection). MC3T3-E1 cells were prepared by adding 10% fetal bovine serum (FBS, fetal bovine serum) and 100 U / mL penicillin ( penicillin), 100U / mL of streptomycin (streptomycin) in cell culture medium at 37°C in humidified CO 2 Incubator (5% CO 2 / 95% air (air)). If the cells fill up about 80% of the culture dish, then utilize phosphate buffered saline (PBS, pH7.4) to wash the monolayer of cells, and process 0.25% trypsin (trypsin)-2.65mM EDTA ( EDTA) for subculture, and the medium was changed every 2 days.

[0075] When MC3T3-E1 cells filled up about 90% of the culture dish, in order to induce cell differentiation, β-glycerophosphate (β-glycerophosphate) (Sigma-Aldrich) (Sigma- Aldrich Co.)) and...

Embodiment 3

[0086] Example 3. Activity evaluation of osteoclasts

[0087] 3-1. Cell culture and differentiation induction

[0088] Raw264.7 cells were purchased from the American Type Culture Collection (ATCC, American Type Culture Collection) as macrophages derived from raw mice. Raw264.7 cells were prepared by adding 10% fetal bovine serum, 100units / mL of penicillin, and 100ug / mL of streptomycin in Dulbecco's modified Eagle's medium (DMEM, dulbecco's modified eagle medium, Welgene Company). primed cell culture medium at 37 °C in a humidified CO 2 Incubator (5% CO 2 / 95% air (air)). If the cells fill up about 80% of the culture dish, wash the monolayer of cells with phosphate buffered saline (PBS, pH 7.4), add cell culture medium to remove the cells for subculture, and replace them every 2 days Medium.

[0089] To induce the differentiation of Raw264.7 cells, 50 ng / mL of receptor activator ligand (RANKL) (Sigma-Aldrich Co.) and 10 μM of PD98059 were added to α-minimum essential medi...

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Abstract

The present invention relates to a composition for prevention and treatment of osteoporosis comprising the extract of medicinal herb mixture. The composition includes an extract of a medicinal herb mixture of Angelica gigas, Paeonia japonica, Atractylodes japonica, Wolfiporia extensa, and Platycodon grandiflorum, and a composition formed by more than one of Gardenia jasminoides, Glycyrrhiza uralensis, Zingiber officinale and Mentha arvensis var. piperascens. The composition is safe in a body, can increase the weight and the length of bones, can increase the bone mineral density, especially, when the optimal content includes the composition of Platycodon grandiflorum, can significantly increase alkaline acid phosphatase of osteoblast, and can significantly inhibit tartrate-resistant acid phosphatase of osteoclast, so as to effectively realize improvement of therapeutic agents for multiple bone diseases, including osteoporosis.

Description

technical field [0001] The present invention relates to containing Chinese angelica (Angelicagigas), white peony root (Paeoniae Radix Alba), Atractylodes macrocephala (Atractylodes macrocephala Koidzumi), Poria cocos (Wolfiporia extensa), gardeniae Fructus (Glycyrrhiza uralensis, Licorice), bellflower (bellflower ( Platycodong grandiflorum A.DC.), dried ginger (Zingiberis Siccatum Rhizoma) and peppermint (Mentha arvensis var. piperascens) mixed extract as active ingredients for the prevention or treatment of bone diseases composition. Background technique [0002] Bone tissue that constitutes our body is a dynamic tissue that undergoes continuous resorption and formation in order to maintain bone mass and skeletal homeostasis. Osteoblasts and osteoclasts play a role in breaking down and growing new bone. Osteoclasts, derived from hematopoietic stem cells, remove old bone through a process of destruction or resorption and participate in maintaining body function by releasing ...

Claims

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Application Information

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IPC IPC(8): A61K36/71A61K36/65A61K36/744A61K36/9068A61P19/10
CPCA23V2002/00A61K36/076A61K36/232A61K36/284A61K36/346A61K36/484A61K36/534A61K36/65A61K36/71A61K36/744A61K36/9068A23L33/105A61K2300/00A23V2200/306A23V2250/21A23V2250/208A23V2300/14A23V2300/10
Inventor 金秀昡俞东辰俞受玎金昌垠全文姬
Owner 金秀昡
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