UPLC method of detecting atractylenolide I and atractylenolide III in atractylodes medicine

Abstract

The invention provides a UPLC method of detecting atractylenolide I and atractylenolide III in atractylodes medicine. The method comprises the following steps: S1, preparation of to-be-measured atractylodes, wherein firstly, the atractylodes is comminuted, and then placed into a triangular flask, methanol is added, and then current solution is placed into an ultrasound device for ultrasound and centrifugation processing, and finally, filtration is carried out; S2, measurement and determination of a standard atractylodes sample, wherein standard atractylodes sample solution is injected into ultra performance liquid chromatography for measurement and determination to obtain a UPLC feature graph of the standard atractylodes sample; and S3, measurement and determination of a to-be-measured atractylodes sample, wherein the measurement and determination solution of the to-be-measured atractylodes sample in the step S1 is injected into the ultra performance liquid chromatography for measurement and determination to obtain a UPLC feature graph of the to-be-measured atractylodes sample, and the to-be-measured atractylodes sample obtained by preparation is quantified through the UPLC featuregraph which is of the standard atractylodes sample and is obtained in the step S2 and the UPLC feature graph of the to-be-measured atractylodes sample. The method provided by the invention can quickly complete analysis testing of the atractylodes sample, and is better in sample precision and recovery of standard addition.

Description

technical field [0001] The invention relates to the technical field of detection, in particular to a UPLC method for detecting atractyloid I and atractyloid III in atractylodes medicine. Background technique [0002] Atractylodes Rhizoma Atractylodes Rhizome is a traditional Chinese medicinal material. It is the dried rhizome of Atractylodes Rhizoma Atractylodes Rhizome. It is a perennial herb. It is warm in nature, sweet in taste and bitter in taste. It is mainly used for spleen deficiency, indigestion, abdominal distension and diarrhea, fatigue and weakness, phlegm retention, dizziness, edema, spontaneous sweating, restless fetal movement, and is an essential medicine for invigorating the spleen in traditional Chinese medicine. Modern pharmacological research shows that Atractylodes macrocephala has the functions of protecting the liver, promoting choleresis, diuresis, antibacterial, anti-inflammatory, anti-tumor, enhancing immunity, lowering blood pressure, lowering blood...

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Problems solved by technology

[0004] There are many literature reports on the determination methods of Atractylodes macrolides in Atractylodes Rhizoma Atractylodes Rhizome, but most of them use GC, HPLC, and mass spectrometry to determine one or several active ingredients. The GC extraction sample loss is large and the extraction steps are cumbersome, and the HPLC analysis period is long. Solvent consumption is large, mass spectrometry equipment is expensive

The quality control of Atractylodes macrocephala has not yet formed a unified standard system, and the 2015 edition of "Chinese Pharmacopoeia" (Part One) has not included the content determination items of Atractylodes macrocephala

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