Method for measuring monosaccharide components of rhizoma atractylodis macrocephala polysaccharide

A technology for polysaccharides and monosaccharides of Atractylodes macrocephala, which is applied in the field of determining the monosaccharide composition of polysaccharides of Atractylodes macrocephala, which can solve the problems of low sensitivity, high cost, and large baseline noise, and achieve the effect of accurate and reliable method, less sample consumption and high sensitivity

Inactive Publication Date: 2018-03-13
FOSHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Since polysaccharides and monosaccharides do not have UV absorption, they cannot be directly measured
When using high performance liquid chromatography to analyze the composition and content of polysaccharides, instruments such as gel chromatographic columns and differential refraction detectors or evaporative light scattering detectors are generally used. These methods not only have low sensitivity, large baseline noise, and column temperature affects the results. Larger, prone to monosaccharide conversion during measurement, and these instruments and columns are expensive

Method used

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  • Method for measuring monosaccharide components of rhizoma atractylodis macrocephala polysaccharide
  • Method for measuring monosaccharide components of rhizoma atractylodis macrocephala polysaccharide
  • Method for measuring monosaccharide components of rhizoma atractylodis macrocephala polysaccharide

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Embodiment 1

[0017] A method for measuring the monosaccharide composition of Atractylodes macrocephala polysaccharide, comprising the steps of:

[0018] 1) Preparation of crude polysaccharide of Atractylodes macrocephala: take the medicinal Atractylodes macrocephala according to the weight ratio of material to liquid: 1:16, extraction time 1.5h, extraction temperature 78°C, extraction times 3 times, after suction filtration, concentrated extract, absolute ethanol The precipitate was concentrated for 12 hours, and the precipitate was vacuum-dried to obtain the crude polysaccharide of Atractylodes macrocephala;

[0019] 2) Preparation of the refined polysaccharide of Atractylodes Rhizome: the crude polysaccharide of Atractylodes Rhizome obtained in step 1) is purified and concentrated with a macroporous resin, and then subjected to vacuum freeze-drying to obtain the refined polysaccharide of Atractylodes Rhizoma;

[0020] 3) Preparation of hydrolyzed samples of Atractylodes macrocephala poly...

Embodiment 2

[0029] A method for measuring the monosaccharide composition of Atractylodes macrocephala polysaccharide, comprising the steps of:

[0030] 1) Preparation of crude polysaccharide of Atractylodes macrocephala: Take the medicinal Atractylodes macrocephala according to the weight ratio of material to liquid: 1:20, extraction time 2h, extraction temperature 75°C, extraction times 3 times, then filter with suction, concentrate the extract, and precipitate with absolute ethanol Concentrate for 12 hours, take the precipitate and dry it under vacuum to obtain the crude polysaccharide of Atractylodes macrocephala;

[0031] 2) Preparation of the refined polysaccharide of Atractylodes Rhizome: the crude polysaccharide of Atractylodes Rhizome obtained in step 1) is purified and concentrated with a macroporous resin, and then subjected to vacuum freeze-drying to obtain the refined polysaccharide of Atractylodes Rhizoma;

[0032] 3) Preparation of hydrolyzed samples of Atractylodes macrocep...

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Abstract

The invention discloses a method for measuring monosaccharide components of rhizoma atractylodis macrocephala polysaccharide. The method comprises the following steps: (1) preparing a rhizoma atractylodis macrocephala polysaccharide hydrolyzed sample; (2) preparing a mixed monosaccharide standard substance; and (3) pre-column derivatization treatment: separately mixing the rhizoma atractylodis macrocephala polysaccharide hydrolyzed sample obtained from step (1) with 0.5 mol / L of a PMP methanol solution and 0.3 mol / L of a NaOH solution, fully shaking, reacting for 30 minutes under the conditionof water bath at the temperature of 70 DEG C, then cooling to the room temperature, adding 0.3 mol / L of hydrochloric acid and neutralizing, adding chloroform and extracting, centrifuging, removing anorganic layer, repeatedly extracting for 2-3 times to obtain water on the upper layer, and filtering with a microfiltration membrane of which the sizes of pores are 0.22 mu m to obtain a rhizoma atractylodis macrocephala polysaccharide derivatization treatment solution and a mixed monosaccharide derivatization treatment solution for subsequent use; and (4) efficient liquid chromatography analysis: determining the monosaccharide components and content of the rhizoma atractylodis macrocephala polysaccharide. The method is high in sensitivity, small in use amount of sample and good in stability;operation is simple; and the monosaccharide components in the rhizoma atractylodis macrocephala polysaccharide are detected at low cost.

Description

technical field [0001] The invention relates to the field of determination methods, in particular to the determination of monosaccharide composition of polysaccharides. Background technique [0002] Traditional Chinese medicine polysaccharides have a wide range of biological activities such as enhancing immunity, anti-stress, and anti-virus. They have been widely used in the field of animal husbandry and veterinary medicine, and are also a hot spot in the research of new veterinary drugs. However, the current status quo is that the quality control standards for veterinary traditional Chinese medicine polysaccharides are not perfect (only the total sugar content is detected), resulting in some fake and inferior polysaccharide products appearing frequently, which endangers the development of the breeding industry. Therefore, it is an important prerequisite for the creation of new polysaccharide drugs or feed additives to carry out research on the quality control of veterinary ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/74
CPCG01N30/06G01N30/74
Inventor 杨鸿王少杰邓桦张勇军巴娟
Owner FOSHAN UNIVERSITY
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