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Rapid asystasiella neesiana tissue culture propagation method

A technology of tissue culture and white bones, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of inability to achieve large-scale reproduction, and achieve the effect of facilitating industrial production

Active Publication Date: 2015-08-05
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The natural reproduction of white elderberry mainly depends on seeds and rhizomes, and each fruit only contains 4 seeds, so it is obviously impossible to achieve the purpose of mass reproduction by natural reproduction

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0043] The difference between this embodiment and Example 1 is that the second MS basal medium adds different components, so as to test the influence of different mediums on the proliferation of adventitious buds:

[0044] Added the second MS basal medium of 6-BA 2.0 mg / L + ZT 0.5 mg / L + IAA 1.5 mg / L, the adventitious bud proliferation rate reached 5.8.

[0045] Added the second MS basal medium of 6-BA 2.0 mg / L + ZT 0.5 mg / L + IBA 0.3 mg / L, the adventitious bud proliferation rate reached 5.95.

[0046] Example 4

[0047] The difference between this embodiment and Example 1 is that the MS basic medium of 1 / 4 strength has different components added, so as to test the influence of different mediums on the rooting rate:

[0048] Added 0.3 mg / L NAA 1 / 4 strength MS basic medium, the rooting rate was 92.8%.

[0049] Added 1 / 4 strength MS medium with IAA 1.0 mg / L, the rooting rate was 92.5%.

[0050] Comparing the foregoing examples, it can be found that Example 1 can finally obt...

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PUM

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Abstract

The invention discloses a rapid asystasiella neesiana tissue culture propagation method which comprises the following steps: (1) inducing multiple shoots, namely, culturing explants taken in March by using an MS basic culture medium with 6-BA+NAA, taking out until the shoots are 3cm, separating axillary buds on two sides along stem cuttings, and transferring and inoculating with a first MS basic culture medium with 6-BA+ZT; (2) enabling adventitious buds to grow and proliferate, namely, cutting for dividing 2-3 multiple shoots generated through induction in the step (1) into one group, and transferring and inoculating into a second MS basic culture medium with 6-BA+ZT+NAA; (3) performing rooting culture of the adventitious buds, namely, cutting the clustered adventitious buds obtained through proliferation culture in the step (2) into single adventitious buds, and transferring and inoculating into an MS basic culture medium with 1 / 4 intensity with NAA; and (4) hardening and transplanting the regenerated seedlings. By adopting the method, asystasiella neesiana regenerated plants can be rapidly obtained, and industrial production can be facilitated.

Description

technical field [0001] The invention belongs to the technical field of tissue culture rapid propagation of plants, in particular to a method for tissue culture rapid propagation of white bone. Background technique [0002] White elderberry [Asystasiellaneesiana (Wall.) Lindau] is a perennial herb of the family Acanthaceae (Acanthaceae), distributed in Jiangsu, Zhejiang, Anhui, Jiangxi, Fujian, Guangdong, Guangxi, Hunan (Zhijiang, Dayong, Yongshun , Yizhang), Hubei, Yunnan, Guizhou, Chongqing, Sichuan and other places, under the forest or by the stream. The whole herb is used as medicine, named white elderberry, jade dragon plate, boneless ramie, jade stem banzhilian, jade elderberry, blood sees sorrow, jade money grass, unicorn grass, jade ring, elderberry, hozen nodal root, gold does not change, rubber grass, Huaxida, Little Ashida, Wood Borers, etc. Harvested in summer and autumn, sun-dried or used fresh. Cool in nature, bitter in the mouth, light, enters lung meridia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 孙骏威朱诚王飞娟丁艳菲江琼蔡冲
Owner CHINA JILIANG UNIV
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