Tissue culture propagation method for stem segments with axillary buds of various cultivated peaches
A technology of axillary buds and stem segments is applied in the field of tissue culture and propagation of axillary bud stem segments of various cultivated peach belts, which can solve the problems of time-consuming, labor-intensive, and high cost, and achieve the effect of reducing costs.
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[0039] ① Acquisition of explants from stem segments with axillary buds of cultivated species
[0040] Select the stem section with axillary buds from the aseptically cultured tissue-cultured seedlings, cut off the stem section with axillary buds about 1-2 cm in length with a sterile blade in an ultra-clean table, and treat the lower end of the morphology as an oblique cut wound for later use. Such as figure 1 , it can be seen that the stem segment with axillary buds is obtained and inoculated in the axillary bud expansion medium;
[0041] ②Induction of axillary bud propagation by stem section with axillary buds
[0042] Inoculate the explants with axillary buds prepared in step ① on the axillary bud expansion medium, induce the growth of axillary buds and produce tillers, and obtain tissue culture plants with multiple axillary buds, such as figure 2 , 3 10 days after the stem segment with axillary buds was inoculated on the axillary bud expansion medium, the axillary buds ...
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