Tissue culture propagation method for stem segments with axillary buds of various cultivated peaches

A technology of axillary buds and stem segments is applied in the field of tissue culture and propagation of axillary bud stem segments of various cultivated peach belts, which can solve the problems of time-consuming, labor-intensive, and high cost, and achieve the effect of reducing costs.

Inactive Publication Date: 2021-06-11
WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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Problems solved by technology

However, in agricultural cultivation, obtaining cultivated seedlings by grafting branches of cultivate...

Method used

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  • Tissue culture propagation method for stem segments with axillary buds of various cultivated peaches
  • Tissue culture propagation method for stem segments with axillary buds of various cultivated peaches
  • Tissue culture propagation method for stem segments with axillary buds of various cultivated peaches

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Embodiment 1

[0039] ① Acquisition of explants from stem segments with axillary buds of cultivated species

[0040] Select the stem section with axillary buds from the aseptically cultured tissue-cultured seedlings, cut off the stem section with axillary buds about 1-2 cm in length with a sterile blade in an ultra-clean table, and treat the lower end of the morphology as an oblique cut wound for later use. Such as figure 1 , it can be seen that the stem segment with axillary buds is obtained and inoculated in the axillary bud expansion medium;

[0041] ②Induction of axillary bud propagation by stem section with axillary buds

[0042] Inoculate the explants with axillary buds prepared in step ① on the axillary bud expansion medium, induce the growth of axillary buds and produce tillers, and obtain tissue culture plants with multiple axillary buds, such as figure 2 , 3 10 days after the stem segment with axillary buds was inoculated on the axillary bud expansion medium, the axillary buds ...

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Abstract

The invention discloses a tissue culture propagation method for stem segments with axillary buds of various cultivated peaches, and belongs to the field of plant rapid propagation and cell engineering. The method includes the steps that (1) explants of the stem segments with the axillary buds of the cultivated species are obtained; (2) propagation induction is carried out on the axillary buds of the stem segments with axillary buds; (3) elongation rooting induction is carried out; (4) seedling hardening and transplanting are carried out; and (5) the explants of the stem segments with the axillary buds of the cultivated peaches is a jujube nectarine or a young stem segment with the axillary buds of Jingyan. The tissue culture propagation method for the stem segments with he axillary buds of the various cultivated peaches has the following advantages and beneficial effects that (1) a working mechanism is clear, scientific and innovative; (2) in-vitro stem segments with axillary buds of two cultispecies, namely the jujube nectarine and the Jingyan, which are high in yield and high in quality are taken as explant materials, and complete tissue culture propagation seedlings are obtained by utilizing the method; and (3) by using the method, a large number of cultivated species tissue culture seedlings can be obtained in a short time, the cost of obtaining cultivated species peach seedlings is reduced to a great extent, and important technical support is provided for obtaining a large number of good varieties in the future.

Description

technical field [0001] The invention belongs to the field of rapid plant propagation and cell engineering, and particularly relates to a method for tissue culture and multiplication of axillary bud stem segments of various cultivated peach scallops. Background technique [0002] Peach belongs to Rosaceae, Prunus, and subgenus Prunus. The cultivated peach is an important fruit tree in the world, and the fruit has important nutritional value. As we all know, peach is a cross-pollination plant, and the genotype of its offspring is quite different from that of woody plants. Therefore, traditionally, grafting techniques are often used to preserve excellent varieties and to be widely used in agricultural production. However, in agricultural cultivation, obtaining cultivated seedlings by grafting branches of cultivated species with rootstock varieties has disadvantages such as time-consuming, labor-intensive, and high cost. How to obtain a large amount of high-quality cultivated s...

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 韩月彭赖恩惠刘晶晶郑蓓蓓
Owner WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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