The invention provides a crop straw based culture medium and a making method thereof. The crop straw based culture medium comprises the following steps that 1, the crop straw moisture is adjusted, beneficial mixed bacteria are added to crop straws, and uniform stirring is performed; 2, material piling is performed, and the material is covered with a black plastic film after piling to perform anaerobic fermentation; 3, the plastic film is uncovered, compound bacteria are scattered, and pile turnover is performed to begin to perform aerobic fermentation; 4, during the aerobic fermentation, super absorbent resin and rare earth nitrate are added, and even stirring is performed to break the material, namely the crop straw based culture medium is obtained. The crop straw based culture medium contains the beneficial bacteria, can maintain soil fertility, effectively restrains root diseases, effectively promotes crop growth, contains the super absorbent resin, improves the water-retention rate and fertilizer-retention rate of soil and is added with the rare earth nitrate to promote crop growth, nitrogen, phosphorus, potassium, calcium, magnesium, organic matter and the like of the crop straws are utilized fully, waste is turned into wealth, and the cost of the medium is reduced.