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Cultivation method and application of flue-cured tobacco regenerated plant

A technique for regenerating plants and cultivation methods, applied in the field of flue-cured tobacco cultivation, which can solve the problems of chlorosis, yellowing and mottled leaves of seedlings, achieve high rooting rate and survival rate, maintain seed quality, and simple operation process

Active Publication Date: 2016-02-10
YUNNAN ACAD OF TOBACCO AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although the operation process of the existing flue-cured tobacco plant tissue culture is not complicated, there are the following disadvantages: the flue-cured tobacco tissue culture process requires strict control of the pollution of bacteria, fungi, etc., and the experimental operating conditions are harsh; in addition, the flue-cured tobacco tissue culture will lead to transparent deformed test tubes The production of seedlings (glass seedlings); the tissue culture of flue-cured tobacco will also cause the whole plant of the seedlings to lose chlorosis, and all or part of the leaves will be yellowed and mottled

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Step (1) Pick 100 top buds in the long -term period (55 days after the roasted tobacco transplanting fields), and the top bud length is 20cm;

[0029] Step (2) The picked top buds or axillary buds soaked 2h at 20 ° C in the root solution of the roots, and the depth depth was 3cm;

[0030] Step (3) Multi -5000ml pottery pots or plastic pots are filled with medium, and the soaked top bud cutting is transplanted to the cultivation substrate to cultivate at 20 ~ 25 ° C.Gently compact to make the culture substrate closely in contact with the base of the top buds or axillary buds. After the cuttings are transplanted, 700ml of nutrients are poured per pot.become;

[0031] 1-7 days after the top bud cutting is transplanted, 2 to 3 layers of sun shading nets cover 70-80%of the light, 8-12 days after cutting transplant, cover 30-40%of light, cut into cuttings for 15 days, after 15 daysUsing natural light.

[0032] Step (4) After 25 days of cutting transplantation, the root inspection...

Embodiment 2

[0034] Step (1) In the middle of the grilled smoke (60 days after the roasted tobacco transplanting fields), 100 axillary buds are taken to the current buds period, and the length of the axillary buds is 9cm;

[0035] Step (2) Soak the raid buds from the root promotion agent solution for 2h at 25 ° C, soak the depth of 2cm;

[0036] Step (3) Multi -5000ml pottery pots or plastic pots are filled with medium, and the soaked top bud cutting is transplanted to the cultivation substrate to cultivate at 20 ~ 25 ° C.Gently compact to make the cultivation substrate closely in contact with the base of the top buds or axillary buds. After the cuttings are transplanted, 650mL2%of the nutrient solution is poured.become;

[0037] After 1-7 days of cutting transplantation, 2 to 3 layers of shading nets cover 70-80%of the light. After cutting transplantation for 8-12 days, cover 30-40%of the light. After 15 days of cutting, natural light is used.Essence

[0038] Step (4) After 25 days of cutting...

Embodiment 3

[0040] Step (1) Pick 100 top buds in the long -term period (45 days after the roasted tobacco transplanting fields), and the top bud length is 15cm;

[0041] Step (2) Picking the top buds or axillary buds soaked for 1h at 25 ° C in the root solution of the roots, and the depth depth is 4cm;

[0042] Step (3) Multi -5000ml pottery pots or plastic pots are filled with medium, and the soaked top bud cutting is transplanted into the medium to cultivate at 25 ° C.Correctly contact the medium with the base of the top buds or the axillary buds. After the cuttings are transplanted, 650ml 5%nutrient solution is poured per pot.

[0043] 1 ~ 7 days after the axillary bud cutting transplant, use 2 to 3 layers of sunshade nets to cover 70-80%of the light, 8 to 12 days after cutting transplant, cover 30-40%of the light, cut into cuttings for 13 days, after 13 days,Use natural light.

[0044] Step (4) Cuttings transplant for 25 ~ 30 days to perform root inspections. Top buds or axillary buds hav...

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Abstract

The invention discloses a cultivation method and application of flue-cured tobacco regenerated plants. The cultivation method of flue-cured tobacco regenerated plants comprises following steps: (1) 45 to 65 days after transplantation of flue-cured tobacco plants to the field, picking off terminal buds or axillary buds of the flue-cured tobacco plants; (2) soaking the picked terminal buds or axillary buds in root growth promoting agent solution; (3)cutting and transplanting the soaked terminal buds or axillary buds to culture substrates and performing cultivation; (4) 25 to 30 days after cutting and transplanting, performing root system checking. If the base part of the terminal buds or axillary buds send forth 8 to 10 adventitious roots, the terminal buds or axillary buds are secondarily transplanted to the field or pots. Then normal management for growth of flue-cured tobacco plants is performed to obtain flue-cured tobacco regenerated plant. According to the cultivation method, terminal buds or axillary buds of flue-cured tobacco plants are adopted and cultured; the components of substrates, light, temperature and humidity are controlled during cultivation process to guarantee the environment is almost identical to adaptive environments of flue-cured tobacco plants. By use of the cultivation method, glass seedlings, yellowing and mottled tobacco are avoided, rooting rate and survival rate are high.

Description

Technical field [0001] The present invention is the field of roasting tobacco cultivation technology, which involves the cultivation method and application of a roasting smoke regenerative plant. Background technique [0002] Tobacco is an important part of my country's tobacco production and one of the important economic crops in my country.Excellent variety is an important production foundation for the development of tobacco production. It is the internal factors of obtaining high -quality tobacco leaves. It is the most economical and effective measure to increase production and quality increase.Tobacco is often used with sexual reproduction, but it is prone to mutation, causing quality degeneration and various diseases, and losing its inherent characteristics. [0003] Plant tissue cultivation technology is based on the regeneration of plant cells and plants. It is part of the planting of the plant through a sterile operation to the medium, and it is cultivated under the condi...

Claims

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Application Information

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IPC IPC(8): A01G31/00A01G9/10
CPCA01G9/0299A01G31/00
Inventor 张晓海李天福许美玲杨春江飞鸿王亚辉杨雪彪尚志强
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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