Tissue culture and rapid propagation method for detoxification of black peel chewing cane

Abstract

The invention discloses a tissue culture and rapid propagation method for detoxification of black peel chewing cane, which comprises the following steps: S1, selecting and treating an explant: S11, selecting, cleaning and soaking robust stalk with buds, embedding the stalk into a peat soil frame in an artificial climate box, cutting off tender axillary buds of black peel chewing cane, soaking the tender axillary buds in an alcohol solution, then soaking the tender axillary buds in a mercury solution, and washing the tender axillary buds with sterile water and draining; S2, a culture stage: S21, taking the tender axillary buds of the black peel chewing cane, putting the tender axillary buds into a primary culture medium for culture, inoculating a proliferation culture medium with cluster buds for proliferation, and transferring the cluster buds to a rooting induction culture medium for rooting culture; and S3, seedling hardening and transplanting stage: transferring the cultured seedlings to natural light for seedling hardening, planting the treated black peel chewing cane seedlings on a loose and breathable seedbed, and dividing the seedlings into single plants when the seedling height is 13-15 cm. Therefore, an excellent single-plant tissue culture seedling production system of the black peel chewing cane is established, a relatively high seedling propagation coefficient is realized, detoxified seedlings with high consistency are obtained, and the method is used for large-scale production and is beneficial to rapid propagation of the seedlings of the non-toxic black peel chewing cane.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, in particular to a rapid propagation method of black-skinned fruit cane through tissue culture for detoxification. Background technique [0002] Sugarcane (Saccharumoficinarum L.) belongs to Poaceae, Saccharum genus. It is not only the most important sugar crop, but also an important energy crop. It plays an important role in my country's sugar industry and energy. Sugarcane has tropical species, Chinese species , Indian species, wild species, river eight kings, succulent flower panicle wild species, etc. Among them, the tropical species is one of the cultivated species, native to the South Pacific, Oceania islands, and later introduced to my country, now Guangdong, Guangxi, Fujian, Hainan A batch of high-yielding, high-sugar, and stress-resistant hybrids with tropical relatives are widely planted in provinces such as Sichuan, Taiwan, and Yunnan. [0003] Sugarcane is a vegetatively prop...

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Problems solved by technology

[0004] At present, the use of this kind of detoxified healthy seedlings in Brazil can increase sugarcane production by 20% to 40% (individual sugarcane areas can increase production by up to 60%), and the sucrose content can increase by more than 0.5% (absolute value). Combined with shoot apex meristem culture, the virus contained in fine sugarcane varieties is effectively removed, and the unity of virus-free and rapid propagation is realized. If sugarcane is to be planted in a large area in a short period of time, it is impossible to use traditional propagation methods , usually a bud section can only reproduce dozens of bud sections a year, if it develops to tens of thousands or hundreds of thousands of acres of planting, it will take many years

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