Tissue culture and rapid propagation method for barthea barthei

Abstract

The invention discloses a tissue culture and rapid propagation method for barthea barthei. The tissue culture and rapid propagation method for the botrychium lanceolatum comprises the steps: S1, selecting barthea barthei superior tree twigs, and cutting into small segments with buds; S2, disinfecting the stem segments, then inoculating the stem segments into a 1/2MS + 0.5 mg/L 6-BA culture medium, setting the room temperature and illumination intensity, and obtaining axillary buds after 30 days; S3, transferring single buds into a WPM + 0.5 mg/L 6-BA culture medium for multiplication culture when the axillary buds grow to 2-3 cm, and obtaining clumpy buds; S4, cutting the clumpy buds into the single buds, transferring the single buds into a 1/2MS + IBA 1.0 mg/L culture medium to be cultured for 30 days, and obtaining tissue culture rooting seedlings; and S5, transplanting the tissue culture rooting seedlings after being hardened for 10-15 days, cleaning the rooting seedlings with well-developed root systems, disinfecting the rooting seedlings with 500-fold carbendazim, and then adopting light-matrix non-woven fabric bags for transplanting. The tissue culture and rapid propagation method for the barthea barthei is high in breeding efficiency and high in survival rate, the obtainedbarthea barthei seedlings are neat and consistent, and excellent characters of stock plants are maintained.

Description

technical field [0001] The invention relates to the technical field of flower propagation, in particular to a method for rapid propagation of flower by tissue culture. Background technique [0002] Barthea barthei is a shrub of the genus Barthea in the family Melastomataceae, and it is produced in Hunan, Guangxi, Guangdong, Fujian and Taiwan. Flowering period is January-April or October-December, cymes, terminal, with 3 flowers, often only 1 mature. Pedicels quadrangular, petals white to pink or purple. It has high ornamental value because of its delicate and beautiful appearance, and it is the only one in the genus Prismus, which is a special product in China. At present, no species has been introduced in domestic gardens, and it has economic value and scientific research value. At present, domestic and foreign researches on prismatic flowers are limited to plant taxonomy and investigation of germplasm resource distribution (Peng Donghui et al., 2007; Bai Yuefeng et al., ...

AI Technical Summary

Problems solved by technology

In the aforementioned propagation methods, the conventional seedlings obtained by seed propagation are often highly differentiated due to their high heterozygosity in the genome, and the quality of the seedlings is uneven. Cutting propagation is easily restricted by excellent mother plants due to the large number of cuttings required.

[0004] In summary, in the prior art, there is no relevant research report on

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