Melaleuca bracteata tissue culture rapid propagation process

A technology of tissue culture rapid propagation and Melaleuca, which is applied in the field of plant tissue culture, can solve the problems of low natural reproduction rate of Melaleuca, seasonal restrictions on cutting propagation, and cumbersome management process in the later stage, so as to achieve easy acquisition and easy seedling hardening. , Cultivate the effect of simple process

Active Publication Date: 2013-12-04
SICHUAN COLORLINK CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Cutting propagation Melaleuca can be propagated by twig cuttings and high-altitude layering methods, but usually more twig cuttings are used. Generally, it is carried out when there is a lot of rain and the night temperature is not too low from April to August. Due to the relative humidity during rooting Large, prone to leaf and stem rot
The natural reproduction rate of Melaleuca is low, cutting propagation is limited by seasons, the later management process is cumbersome, the survival rate of cutting seedlings is low, and it is difficult to scale production

Method used

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  • Melaleuca bracteata tissue culture rapid propagation process
  • Melaleuca bracteata tissue culture rapid propagation process
  • Melaleuca bracteata tissue culture rapid propagation process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Melaleuca tissue culture rapid propagation method, it may further comprise the steps:

[0022] S1. Sterilize the explants, collect the young and tender stems of Melaleuca officinalis without damage by diseases and insect pests, remove the excess leaves, rinse in running water for more than half an hour, then soak in detergent for 10 minutes, and finally rinse with running water until no foam appears, Then put it on the ultra-clean workbench, treat it with alcohol for 20 seconds, wash it once with sterile water, treat it with mercury chloride for 5 minutes, and rinse it with sterile water five times;

[0023] S2, start the culture, cut the sterilized explants to 2 ~ 3cm young stems, inoculate in the starting medium, the starting medium is MS+6-BA 0.5mg / L+sucrose 30g / L+agar 5g / L, pH adjusted to 5.8-6.2, cultivated in a culture room with a light intensity of 3000LX, a light time of 16h / d, and a temperature of 24°C. After 2-3 weeks of culture, the axillary buds grow 2-4cm,...

Embodiment 2

[0028] Melaleuca tissue culture rapid propagation method, it may further comprise the steps:

[0029] S1. Sterilize the explants, collect the young and tender stems of Melaleuca officinalis without damage by diseases and insect pests, remove the excess leaves, rinse in running water for more than half an hour, then soak in detergent for 8 minutes, and finally rinse with running water until no foam appears, Then put it on the ultra-clean workbench, treat it with alcohol for 15 seconds, wash it once with sterile water, treat it with mercury chloride for 7 minutes, and rinse it with sterile water six times;

[0030] S2, start the culture, cut the sterilized explants to 2 ~ 3cm young stems, inoculate in the starting medium, the starting medium is MS+6-BA 0.5mg / L+sucrose 30g / L+agar 5g / L, pH adjusted to 5.8-6.2, cultured in a culture room with a light intensity of 3000LX, a light time of 15h / d, and a temperature of 26°C. After 2-3 weeks of culture, the axillary buds grow 2-4cm, as ...

Embodiment 3

[0035] Melaleuca tissue culture rapid propagation method, it may further comprise the steps:

[0036] S1. Sterilize the explants, collect the young and tender stems of Melaleuca gold without pests and diseases, remove the excess leaves, rinse in running water for more than half an hour, then soak in detergent for 12 minutes, and finally rinse with running water until no foam appears. Then put it on the ultra-clean workbench, treat it with alcohol for 25 seconds, wash it once with sterile water, treat it with mercury chloride for 3 minutes, and rinse it with sterile water four times;

[0037] S2, start the culture, cut the sterilized explants to 2 ~ 3cm young stems, inoculate in the starting medium, the starting medium is MS+6-BA 0.5mg / L+sucrose 30g / L+agar 5g / L, pH adjusted to 5.8-6.2, cultivated in a culture room with a light intensity of 3000LX, a light time of 17h / d, and a temperature of 22°C. After 2-3 weeks of culture, the axillary buds grow 2-4cm, as figure 1 shown;

...

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Abstract

The invention discloses a melaleuca bracteata tissue culture and rapid propagation process, which comprises the following steps: S1. carrying out explant sterilization, S2. starting culture: cutting the sterilized explants to 2-3cm tender stems, and inoculating the stems in a start-up culture medium for culture; S3. carrying out primary culture: cutting off auxiliary buds and transferring the auxiliary buds to a primary culture medium for culture; S4. carrying out secondary culture: cutting off the maxillary buds and transferring the auxillary buds to a secondary culture medium for culture; and S5. transplanting hardened plantlets: directly transplanting tissue cultured melaleuca bracteata plantlets striking shoot and being robust in a greenhouse seedbed after the culture medium is washed off. The melaleuca bracteata tissue culture and rapid propagation process has the beneficial effects that using the tissue culture technology to obtain the melaleuca bracteata tissue culture plantlets, the culture medium formula is simple, the culture process flow is simple and quick, the operation is simple and convenient, the propagation coefficient is relatively high, and melaleuca bracteata aseptic plantlets can be easily obtained, and the hardened plantlets are easy to survive; and the melaleuca bracteata tissue culture and rapid propagation process is time-saving and labor-saving, and provides technical support for industrialized seedling production of melaleuca bracteata and deep processing thereof.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for tissue culture and rapid propagation of melaleuca. Background technique [0002] Melaleuca bracteata (Melaleuca bracteata) is an evergreen tree of the Myrtaceae Melaleuca genus, also known as Golden Treasure Tree, Golden Fragrant Willow, and Golden String Willow. It is a new variant tree species selected and bred in the early 1990s. Introduced to China for the first time. This tree has a beautiful tree shape, upright main stem, red twigs, easy to trim and shape, fast growth, golden or goose yellow leaves all year round, fragrant smell, can withstand low temperature of -4°C and high temperature of 42°C, and is both drought and resistant Waterlogged, suitable for growing near water, resistant to salinity and strong wind, it is one of the most popular new tree species with the best visual effect in the world. , cityscapes, etc. In addition to the great o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 冯爱云马建华王小辉晏强罗丽君
Owner SICHUAN COLORLINK CO LTD
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