Method for Rapid Propagation of Phalaenopsis by Utilizing Pedicel Segments After Cutting Buds to Induce Cluster Buds
A technology for phalaenopsis and flower stalks, which is applied in the field of phalaenopsis reproduction, can solve the problems of low production volume of commercial seedlings, slow plant reproduction rate, and difficulty in germination, and achieves improved reproduction rate and production volume, facilitates factory management, and saves production costs. Effect
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[0024] (1) selection of explant: choose the Phalaenopsis cultivar black jack plant that does not have damage by disease and insect, treat that flower withers, flowering period has passed, with scissors the whole pedicel is cut off, cuts the long pedicel axillary bud stem segment of 3-4cm (one section one Bud) is explant material;
[0025] (2) Preparation of culture medium:
[0026] Bud induction medium: MS+6-BA2.5mg / L+NAA0.5mg / L, sucrose 20g / L, pH 5.6:
[0027] Bud proliferation medium: MS+6-BA5.0mg / L+NAA0.5mg / L, sucrose 20g / L, pH 5.6:
[0028] Rooting and strong seedling medium: 1 / 2MS+NAA1.0mg / L+banana puree 100g / L, sucrose 20g / L, pH 5.6;
[0029] (3) Disinfection treatment of explants: After rinsing the above explant materials under tap water for 30-60 minutes, put them into a 75% alcohol solution in an ultra-clean workbench for 45 seconds, then blot them dry with sterile filter paper Alcohol, sterilized in 7-10% NaClO disinfectant for 12-15min, aged material sterilized f...
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