Disinfection method for pedicel buds of phalaenopsis amabilis

Abstract

The invention provides a disinfection method for pedicel buds of phalaenopsis amabilis, which comprises the steps of: 1) selecting phalaenopsis amabilis plants which have complete pedicel buds and remains pedicels with the pedicel buds; 2) wiping the pedicels with alcohol cotton balls for 3-5 times from bottom to top; 3) according to the aged degree of the pedicels, classifying the pedicels into aged pedicels and tender pedicels; 4) preparing three disinfectants, respectively placing the aged pedicels and tender pedicels into two empty sterile bottles, pouring 1# disinfectant into the bottles with the aged pedicels and the 2# disinfectant into the bottles with the tender pedicels, and then pouring the disinfectants out; 5) stripping off the bracts on the pedicel buds on the aged pedicels and the tender pedicels after the first disinfection, soaking the pedicel buds, after the bracts are stripped off, in the 3# disinfectant, and pouring the disinfectant out; 6) inoculating the pedicel buds in a culture bottle filled with a pedicel bud inductive culture medium; and 7) placing the culture bottle in a culture room and performing induction to the pedicel buds. The disinfection method is 2-7% in pollution ratio of the pedicel buds, is 95-99% in survival rate of the pedicel buds, and is low in cost of the disinfectants.

Description

technical field

[0001] The invention relates to a method for cultivating and disinfecting pedicel buds, in particular to a method for disinfecting phalaenopsis pedicel buds. Background technique

[0002] At present, most Phalaenopsis tissue culture producers in China use sodium hypochlorite solution or mercuric chloride solution to disinfect the phalaenopsis buds. The mercuric chloride solution has a very good disinfection effect on the pedicel buds, but it contains harmful substances to the environment and soil. Seriously polluting mercury, so many responsible manufacturers have abandoned it. Sodium hypochlorite solution has a good disinfection effect, is easy to clean, and has no pollution. It is the main disinfectant used by domestic Phalaenopsis tissue culture manufacturers. At present, the pollution rate of sodium hypochlorite for disinfection of Phalaenopsis pedicel buds is about 20%. Sodium hypochlorite is a chemical reagent. Buy it Need to go to a specialized chemic...