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Butterfly orchid tissue culturing method

A technique of Phalaenopsis and tissue culture, which is applied in the field of plant tissue culture, can solve the problems of no formation of Phalaenopsis, no involvement in the rooting and transplanting of Phalaenopsis tissue culture seedlings, and failure to provide a basis for production, so as to achieve simple operation and low cost. cheap effect

Inactive Publication Date: 2015-06-10
芜湖东源新农村开发股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the in vitro rapid propagation research of Phalaenopsis plants is mostly concentrated on peony, and there are few domestic and foreign researches on Phalaenopsis tissue culture. There is no complete set of methods for rapid propagation of Phalaenopsis underground buds, nor does it involve the rooting and transplanting of Phalaenopsis tissue culture seedlings, which cannot provide a basis for production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for tissue culture of Phalaenopsis, comprising the steps of obtaining Phalaenopsis shoot tips, sterilizing and disinfecting Phalaenopsis shoot tips, and carrying out primary culture, subculture and rooting culture of the sterilized Phalaenopsis shoot tips, characterized in that :

[0026] (1) Phalaenopsis stem tip acquisition:

[0027] Cut the newly germinated buds of Phalaenopsis in autumn, wash them with pure water for 30 minutes, and then put them on the ultra-clean workbench;

[0028] (2) Phalaenopsis stem tip sterilization:

[0029] Soak in 70% alcohol for 60 seconds, then soak in 1:3 84 disinfectant diluted with water for 12 minutes, and blot the surface moisture;

[0030] (3) Primary culture of Phalaenopsis shoot tips:

[0031] 1) The tissue-cultured seedlings obtained from the subculture were refrigerated at 3°C ​​for 8 days, then transferred to the rooting medium containing IBA and cultured for 15 days;

[0032] 2) Transfer the tissue-cultured seed...

Embodiment 2

[0043] A method for tissue culture of Phalaenopsis, comprising the steps of obtaining Phalaenopsis shoot tips, obtaining and sterilizing Phalaenopsis shoot tips, and carrying out primary culture, subculture and rooting culture of the sterilized Phalaenopsis shoot tips, which are characterized in in:

[0044] (1) Phalaenopsis stem tip acquisition:

[0045] Cut the newly germinated buds of Phalaenopsis in autumn, wash them with pure water for 30 minutes, and then put them on the ultra-clean workbench;

[0046] (2) Phalaenopsis stem tip sterilization:

[0047] Soak in 75% alcohol for 30 seconds, then soak in 1:3 84 disinfectant diluted with water for 12 minutes, and dry the surface moisture;

[0048] (3) Primary culture of Phalaenopsis shoot tips:

[0049] 1) The tissue-cultured seedlings obtained from subculture were refrigerated at 3-6°C for 8-10 days, then transferred to the rooting medium containing IBA and cultured for 15 days;

[0050] 2) Transfer the tissue-cultured se...

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PUM

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Abstract

The invention discloses a butterfly orchid tissue culturing method. The method comprises the steps of obtaining butterfly orchid stem tips, sterilizing the butterfly orchid stem tips, conducting preliminary culturing and subculturing to the butterfly orchid stem tips obtained after sterilization, and conducting rooting culturing. The method has the advantages that by means of the method, the rooting percentage of butterfly orchid tissue culture seedling plants reaches 26.7% at the maximum; tissue culture seedlings which are generated by the adoption of the stem tips and cultured in an isolated mode are capable of maintaining excellent characteristics of an original variety, so that foundations of elite propagation and industrial production are laid; according to the butterfly orchid tissue culturing method, the operation is simple, the cost is low, and the method is applicable to intermediate propagation of majority of ornamental butterfly orchids.

Description

technical field [0001] The invention relates to plant tissue culture technology, in particular to a method for tissue culture of Phalaenopsis. Background technique [0002] Phalaenopsis aphrodite Rchb. F. belongs to the genus Phalaenopsis Phalaenopsis. It is native to subtropical rainforest and is an epiphytic orchid. The thick white aerial roots of Phalaenopsis are exposed around the leaves. In addition to absorbing nutrients in the air, they also have growth and photosynthesis. In the Spring Festival, the Phalaenopsis plant has long flower stalks from the leaf axils and blooms like butterflies flying. It is favored by flower lovers and is known as the "Queen of the Orchid". [0003] At present, the in vitro rapid propagation research of Phalaenopsis plants is mostly concentrated on peony, and there are few domestic and foreign researches on Phalaenopsis tissue culture. There is no complete set of methods for rapid propagation of Phalaenopsis underground buds, nor doe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 汪锡文
Owner 芜湖东源新农村开发股份有限公司
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