Production technology of edible mushroom liquid spawn by adopting reducing process

Abstract

The invention relates to the field of biological engineering, in particular to a production technology of liquid spawn. The production technology of edible mushroom liquid spawn by adopting a reducing process comprises the following steps of: 1) cultivating, carrying out liquid culture on the edible mushroom liquid spawn on a liquid nutrient medium to obtain mycelium; 2) obtaining a spawn block, namely carrying out pure culture on the mycelium on a PDA (Potato Dextrose Agar) culture medium to obtain the spawn block of the mycelium; 3) smashing, namely smashing the spawn block by adopting a spawn block smashing machine; and 4) diluting, namely diluting the smashed spawn block with sterile water. By adopting the technical scheme, the production technology provided by the invention has the significant advantages of high mycelium content, short liquid spawn cultivation time, less equipment investment, low cost and the like.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a production process of strains. Background technique [0002] In the artificial cultivation of fungi such as Flammulina velutipes, crab-flavored mushrooms, gray tree mushrooms, slide mushrooms, and king oyster mushrooms, strains are used. In the production process of strains, there are two methods of solid culture and liquid culture. Solid culture is more common, but the operation of solid culture has problems such as complicated culture, time-consuming and laborious, and inability to confirm the quality of bacteria. Therefore, liquid culture has attracted much attention. The production process of "mother species, first-level shake flask species, second-level shaker flask species, and incubator" is mostly used in the conventional preparation of liquid strains. The above-mentioned production process has many links, complicated operation, long cycle, Defects such as high cost are n...

AI Technical Summary

Problems solved by technology

Solid culture is more common, but the operation of solid culture has problems such as complicated culture, time-consuming and labor-intensive, and inability to confirm the quality of the bacteria.

Therefore, liquid culture has attracted much attention. The production process of "mother species, first-level shake flask species, second-level shaker flask species, and incubator" is mostly used in the conventional preparation of liquid strains. The above-mentioned production process has many links, complicated operation, long cycle, Defects such as high cost are not conducive to mass promotion and application

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