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Liquid fermentation culture medium for Grifola frondosa and preparation method of liquid fermentation culture medium

A technology of liquid fermentation and culture medium, applied in the direction of liquid fertilizer, fertilizer made of biological waste, application, etc., can solve the problems of not being able to adapt to market competition, poor differentiation of fruiting bodies, and many times of transfer, so as to achieve the goal of not being easily infected with miscellaneous Bacteria, low price, short fermentation cycle

Inactive Publication Date: 2017-01-11
山东省科创食用菌产业技术研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to records, in the 1980s and 1990s, many domestic units and individuals organized many times to explore the artificial domestication and cultivation of Grifola frondosa, and obtained pure strains, but the fruiting bodies were not well differentiated, and the yield was low, so there was no success
In the early 1990s, some domestic units used solid strains to imitate wild cultivation methods. Although the biological efficiency reached 80%, there were generally problems such as long production cycle, many pollution opportunities, high number of transfers, and easy degradation of strains. The production technology is far from being able to adapt to the fierce market competition and cannot compete with the same industry in the world

Method used

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  • Liquid fermentation culture medium for Grifola frondosa and preparation method of liquid fermentation culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Grifola frondosa liquid culture medium contains wheat bran 15g, glucose 10g, KH 2 PO 4 2.5g, MgSO 4 ·7H 2 O1.8g, corn flour 5.5g, peptone 3.5g, CaCl 2 0.8g, V B1 0.12g, corn silk juice 40mL, the rest is distilled water;

[0021] The preparation method of Grifola frondosa liquid culture medium is:

[0022] (1) Add 15g of wheat bran and 5.5g of corn flour into 800mL of distilled water, boil for 20-30min, filter to obtain the filtrate;

[0023] (2) Add 10g of glucose and KH to the filtrate 2 PO 4 2.5g, MgSO 4 ·7H 2 O1.8g, peptone 3.5g, CaCl 2 0.8g, V B1 0.12 g and 40 ml of corn silk juice were stirred evenly to completely dissolve the added raw materials, then added distilled water to 1000 mL, sterilized at 121°C for 20 minutes, and cooled to obtain a Grifola frondosa liquid fermentation medium.

[0024] The preparation method of corn silk juice is as follows: take 20 g of dried corn silk, add 100 ml of distilled water to boil for 30 minutes, filter, and dilute...

Embodiment 2

[0026] Grifola frondosa liquid medium: Each liter of medium contains 10g of wheat bran, 15g of glucose, KH 2 PO 4 1.8g, MgSO 4 ·7H 2 O2.2g, corn flour 4.8g, peptone 2.5g, CaCl 2 1.2g, V B1 0.08g, corn silk juice 55mL, the balance is distilled water;

[0027] (1) Preparation of Grifola frondosa liquid medium: add 10g of wheat bran and 4.8g of corn flour to 800mL of distilled water, boil for 20-30min, filter to obtain the filtrate;

[0028] (2) Add 15g of glucose and KH to the filtrate 2 PO 4 1.8g, MgSO 4 ·7H 2 O 2.2g, peptone 2.5g, CaCl 2 1.2g, V B1 0.08g and 55ml of corn silk juice were stirred evenly to dissolve the raw materials completely, then added distilled water to 1000mL, sterilized at 121°C for 30min, and cooled to obtain the Grifola frondosa liquid fermentation medium.

[0029] Wherein the preparation method of corn silk juice is: take 25g of dry corn silk, add 100ml of distilled water to boil for 30min, filter, and the filtrate is fixed to 100ml to get fi...

Embodiment 3

[0031] Grifola frondosa liquid medium: Each liter of medium contains 12g of wheat bran, 13g of glucose, KH 2 PO 4 2g, MgSO 4 ·7H 2 O2g, corn flour 5g, peptone 3g, CaCl 2 1g, V B1 0.1g, corn silk juice 50mL, the rest is distilled water;

[0032] (1) Preparation of Grifola frondosa liquid medium: add 12g of wheat bran and 5g of corn flour into 800mL of distilled water, boil for 30min, filter to obtain the filtrate;

[0033] (2) Add 13g of glucose and KH to the filtrate 2 PO 4 2g, MgSO 4 ·7H 2 O 2g, peptone 3g, CaCl 2 1g, V B1 Stir 0.1 g and 50 ml of corn silk juice evenly to dissolve the raw materials completely, add distilled water to 1000 mL, sterilize at 121° C. for 30 min, and cool to obtain the Grifola frondosa liquid fermentation medium.

[0034] Wherein the preparation method of corn silk juice is: take 25g of dry corn silk, add 100ml of distilled water to boil for 30min, filter, and the filtrate is fixed to 100ml to get final product;

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Abstract

The invention belongs to the technical field of fermentation culture of edible mushrooms and particularly relates to a liquid fermentation culture medium for Grifola frondosa and a preparation method of the liquid fermentation culture medium. The liquid fermentation culture medium contains the following components: wheat bran, glucose, KH2PO4, MgSO4.7H2O, corn flour, peptone, CaCl2, VB1, corn stigma juice and the balance of distilled water, wherein the corn stigma juice is prepared by the steps of adding dried corn stigma into distilled water, boiling, filtering, and carrying out volume fixation on filtrate. The preparation method comprises the following steps: adding the wheat bran and the corn flour into distilled water, boiling, filtering to obtain filtrate, adding glucose, KH2PO4, MgSO4.7H2O, peptone, CaCl2, VB1 and the corn stigma juice, uniformly stirring, carrying out sterilization, and cooling, so as to obtain the liquid fermentation culture medium. The liquid fermentation culture medium for the Grifola frondosa has the characteristics that the conversion efficiency is high, the culture period is short, the infectious microbe infection is unlikely to occur, and the like, and furthermore, the yield of mycelium pellets of the Grifola frondosa is increased.

Description

technical field [0001] The invention belongs to the technical field of edible fungus fermentation and cultivation, and in particular relates to a grifola frondosa liquid fermentation medium and a preparation method thereof. [0002] technical background [0003] Grifola frondosa is a kind of edible fungus with both medicine and food, belonging to Basidiomycotina, Laminomycetes, Non-sepibasidiomycetes, Non-Pleatellales, Polyporaceae, Tree Flower (also known as Grifola) genus. Grifola frondosa has many names, such as polypore, thousand Buddha, chestnut mushroom, lotus mushroom, Grifola, maitake and so on. Wild Grifola frondosa is distributed in Japan, Europe, North America and many areas of my country, such as Heilongjiang, Jilin, Hebei, Sichuan, Yunnan, Guangxi, Fujian and other provinces and regions. In recent years, domestic Grifola frondosa has been cultivated in some provinces such as Hebei, Zhejiang, Fujian and other provinces for fresh consumption or extraction of funct...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G1/00
CPCC05B7/00C05G5/20C05D3/00C05D5/00C05F5/002C05F11/00C05F11/10
Inventor 李晓博高霞任鹏飞刘孝利
Owner 山东省科创食用菌产业技术研究院
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