A method for fermenting and producing Fidaxomycin
A technology of fidaxomicin and fermentation liquid, applied in the field of microbial fermentation to produce antibiotics, can solve the problems of lack of late-acting carbon and nitrogen sources in the medium, low yield of fermentation products, poor fermentation effect, etc., and increase the utilization rate of the medium , increase fermentation yield, mild condition effect
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Embodiment 1
[0029] Example 1 Batch-type addition of edible oil during fermentation
[0030] Prepare 300L seed medium in a 500L seed tank, sterilize at 120°C for 30 minutes, cool to 27°C, inoculate 48h seed-age shake flask seeds at an inoculum volume ratio of 0.1% of the seed medium volume, and incubate at 27°C for 28h.
[0031] Prepare 3500L fermentation medium in a 5000L fermenter, sterilize at 120°C for 30 minutes, then cool to 27°C, insert the vegetative inoculum into the above seed medium with an inoculation amount of 4% by volume of the fermentation medium, and control the temperature during the fermentation process The temperature is 27°C, the control pressure is 0.04Mpa, the control ventilation is 0.5VVM, and the control speed is 100rpm to ensure that the dissolved oxygen is above 30%.
[0032] The fermentation culture is continued, and after 48 hours, the dissolved oxygen in the fermenter drops to 32%, and gradually begins to rise again, and soybean oil is added to the fermenter f...
Embodiment 2
[0033] Example 2 Continuous Addition of Edible Oil During Fermentation
[0034] Prepare 100L seed medium in a 200L seed tank, sterilize at 120°C for 30 minutes, cool to 27-29°C, insert 48h seed-age shake flask seeds at an inoculum volume ratio of 0.3% of the seed medium volume, and then incubate at 29°C for 36h .
[0035] Prepare 1400L fermentation medium in a 2000L fermenter, sterilize at 120°C for 30 minutes and then cool to 29°C, add the vegetative inoculum in the above seed medium according to the inoculum volume ratio of 8% of the fermentation medium volume, and control the temperature during the fermentation process The temperature is 29°C, the control pressure is 0.06Mpa, the control ventilation is 1.5VVM, and the control speed is 250rpm to ensure that the dissolved oxygen is above 30%.
[0036] Continue the fermentation culture. After 64 hours, the dissolved oxygen in the fermenter drops to 33%, and gradually starts to rise again. Take 24 hours as a time unit, and bet...
Embodiment 3
[0037] Example 3 Adding edible oil intermittently during the fermentation process
[0038] Prepare 100L seed medium in a 200L seed tank, sterilize at 120°C for 30 minutes, then cool to 28°C, inoculate 48h-old shake flask seeds at an inoculum volume ratio of 0.2% of the seed medium volume, and then incubate at 28°C for 30h.
[0039] Prepare 700L fermentation medium in a 1000L fermenter, sterilize at 120°C for 30 minutes and then cool to 28°C, insert the vegetative inoculum into the above seed medium with an inoculation amount of 5% by volume of the fermentation medium, and control the temperature during the fermentation process At 28°C, control the pressure at 0.05Mpa, control the ventilation at 1.0VVM, and control the speed at 200rpm to ensure that the dissolved oxygen is above 30%.
[0040]The fermentation culture is continued, and the dissolved oxygen in the fermenter drops to 30% after 52 hours, and gradually begins to rise again. Add rapeseed oil to the fermenter every tim...
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