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Synergist and medium for fermentation culture of hairy mould
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A technology of fermentation culture and synergist, applied in the direction of microorganism-based methods, microorganisms, fungi, etc., can solve the problems of long fermentation time, low metabolic rate, unstable products, etc.
Active Publication Date: 2013-06-26
DINGZHENG XINXING BIOTECH TIANJIN
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Problems solved by technology
However, the disadvantages of poor stability, low metabolic rate, long fermentation time and unstable products during the fermentation process of Mucor have limited the further application of Mucor.
Method used
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Embodiment 1
[0026]
[0027] Carry out the fermentation culture of Mucor:
[0028] 1. Prepare synergist and culture medium, then mix the two, the amount of synergist added is 1% of the total weight of the medium.
[0029] 2. The mucormycetes are selected from the mucormycetes, which are activated and inoculated into the culture medium. The culture temperature is 30 degrees Celsius, the culture time is 36 hours, and the song is turned every 4 hours.
[0030] 3. Purifying the fermentation broth in step 2, and performing an enzyme activity test on the separated product.
[0031] While performing steps 1-3, select the same mucormycetes to culture in the traditional medium for the same time, and the isolated product is also tested for enzyme activity.
Embodiment 2
[0033]
[0034] Carry out the fermentation culture of Mucor:
[0035] 1. Prepare synergist and medium, then mix the two, and the amount of synergist added is 0.75% of the total weight of the medium.
[0036] 2. Mucorus racemosa is selected for mucormycetes, and after activation, it is inoculated into the culture medium, the culture temperature is 40 degrees Celsius, the culture time is 36 hours, and the song is turned every 6 hours.
[0037] 3. Purifying the fermentation broth in step 2, and performing an enzyme activity test on the separated product.
[0038] While performing steps 1-3, select the same mucormycetes to culture in the traditional medium for the same time, and the isolated product is also tested for enzyme activity.
Embodiment 3
[0040]
[0041] Carry out the fermentation culture of Mucor:
[0042] 1. Prepare synergist and culture medium, then mix the two, the amount of synergist added is 0.5% of the total weight of the medium.
[0043] 2. The mucormycetes are selected from Mucor ruckeri, activated and inoculated into the culture medium, the culture temperature is 50 degrees Celsius, the culture time is 48 hours, and the song is turned every 5 hours.
[0044] 3. Purifying the fermentation broth in step 2, and performing an enzyme activity test on the separated product.
[0045] While performing steps 1-3, select the same mucormycetes to culture in the traditional medium for the same time, and the isolated product is also tested for enzyme activity.
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Abstract
The invention relates to a synergist for fermentation culture of hairy mould. The synergist is prepared from the following raw materials in part by weight: 0.05 to 0.33 part of forchlorfenuron, 0.10 to 0.20 part of lysine, 0.10 to 0.25 part of cysteine, 0.15 to 0.25 part of tryptophan, 0.50 to 1.50 parts of vitamin B12, 0.50 to 1.20 parts of vitamin B6, 0.30 to 0.80 part of choline chlorideand 0.80 to 1.50 parts of L-threonine. The synergist prepared by mixing the forchlorfenuron, the lysine, the semi amic acid, the tryptophan, the vitamin B12, the vitamin B6, the cholinechloride and the L-threonine in a certain ratio into a medium, so that the hypha content during fermentation of the hairy mould is greatly increased compared with that of the traditional medium; and moreover, the enzymatic activity of a fermentation product is obviously higher than that of the traditional medium. Therefore, after the medium containing the synergist is adopted, the hypha content of the hairy mould and the activity of a metabolin are superior to those of the traditional medium, and the medium has good economic, ecological and social benefits.
Description
technical field [0001] The invention relates to the technical field of mucormycetes cultivation, in particular to a synergist and culture medium for mucormycetes fermentation and cultivation. Background technique [0002] Mucor is also called black mold and long-tailed mold. It reproduces by cystspores and zygospores. It exists in the environment such as manure, grass and air, and grows well under the conditions of high temperature, high humidity and poor ventilation. Mucor has a wide range of uses and can produce a variety of metabolites. It is widely used in industrial production, such as using its amylase to make koji and wine, and using its protease to brew fermented bean curd and tempeh. [0003] After mucormycetes is activated, it is inoculated in the medium, and after cultivating for a certain period of time, the fermentation broth is purified to obtain various metabolites and other antibiotics. The whole cultivation process has the advantages of simple raw materials...
Claims
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Application Information
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