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Method for culturing solid cordyceps militars strain

A technology of solid strains and cultivation methods, which is applied in the direction of fungi, etc., can solve the problems of difficulty in ensuring the consistency of the inoculation amount, long time for color change, and poor resistance of miscellaneous bacteria, so as to achieve short time for color change and shorten production Cycle, strong resistance effect

Inactive Publication Date: 2011-04-06
HESHAN ZHONGCHUN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The solid strain spore of Cordyceps militaris cultivated like this is less, and activity is low, and the resistance to miscellaneous bacterium is poor, thus the liquid strain of Cordyceps militaris solid strain amplification, mycelial content in the unit liquid culture medium, activity is general, long-term Stratification is easy to occur when standing still, and it is difficult to ensure the consistency of the inoculum amount during inoculation. After the liquid strain is inoculated into the production medium, the colony grows slowly and the color change takes a long time

Method used

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  • Method for culturing solid cordyceps militars strain
  • Method for culturing solid cordyceps militars strain

Examples

Experimental program
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Embodiment 1

[0019] The selected Cordyceps militaris strain is Cm001, which was purchased from the Edible Fungus Laboratory of the School of Biology, China Agricultural University. The cultivation process includes the following steps:

[0020] 1) Prepare common slant solid medium, the ratio of the medium is: Water: 7500g Potato: 1400g Glucose: 180g Dipotassium hydrogen phosphate: 15g Magnesium sulfate: 5g Peptone: 20g Silkworm chrysalis powder: 18g Agar powder: 75g, will prepare The culture medium is packed well, at 121 ℃, pressure 1.05kg / cm 2 Sterilize under conditioned conditions for 40 minutes, cool to room temperature, and set aside;

[0021] 2) Inoculation: under sterile conditions, inoculate the Cm001 strain into the culture medium cooled to room temperature;

[0022] 3) Dark excitation culture: culture the inoculated strains in the shade at 23°C for 3 days, lower the temperature to 20°C, and continue the shade culture for 4 days;

[0023] 4) Light excitation: Culture the strains c...

Embodiment 2

[0034] The selected Cordyceps militaris strain is Cordyceps militaris C-2, which was purchased from the Edible Fungi Research Institute of Yutai County, Shandong Province. The cultivation process includes the following steps:

[0035] 1) Prepare common slant solid medium, the ratio of the medium is:

[0036] Water: 9000g Potatoes: 1700g Glucose: 200g Dipotassium hydrogen phosphate: 20g Magnesium sulfate: 7g Peptone: 26g Silkworm chrysalis powder: 20g Agar powder: 90g 2 Sterilize under conditioned conditions for 40 minutes, cool to room temperature, and set aside;

[0037] 2) Inoculation: under aseptic conditions, inoculate Cordyceps militaris C-2 bacteria into the culture medium cooled to room temperature;

[0038] 3) Dark excitation culture: culture the inoculated strains in the shade at 24°C for 3 days, lower the temperature to 21°C, and continue the shade culture for 4 days;

[0039] 4) Light excitation: Culture the strains cultured under dark excitation for 24 h...

Embodiment 3

[0050] The selected strain is C311, which was purchased from Professor Shi Mubiao, School of Food Science, South China Agricultural University. The cultivation process includes the following steps:

[0051] 1) Prepare common slant solid medium, the ratio of the medium is: Water: 8500g Potato: 1650g Glucose: 195g Dipotassium hydrogen phosphate: 19g Magnesium sulfate: 6.5g Peptone: 25g Silkworm chrysalis powder: 21g Agar powder: 87.5g;

[0052] 2) The prepared medium is divided into packages, at 121°C, pressure 1.05kg / cm 2 Sterilize under conditioned conditions for 40 minutes, cool to room temperature, and set aside;

[0053] 3) Inoculation: under sterile conditions, inoculate C311 bacteria in the culture medium cooled to room temperature by conventional method;

[0054] 4) Dark excitation culture: culture the inoculated strains in the shade at 25°C for 3 days, lower the temperature to 23°C, and continue the shade culture for 4 days;

[0055] 5) Light excitation: Cult...

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Abstract

The invention discloses a method for culturing solid cordyceps militars strain, which comprises the following steps: inoculating the cordyceps militars strain in a solid slope culture medium, culturing for three days in a light-proof environment at 23-25 DEG C, then lowering the temperature to 20-23 DEG C, continuing the light-proof excitation culture for four days and then culturing for 24 hoursin natural floodlight. The solid cordyceps militars strain cultured by the method has more thriving aerial hypha, which is over 1.5 times larger than the aerial hypha cultured by general methods, with more virgations and less probability of rupture; the lawn is more sturdy and fleshier, with bright yellow color; the spore is larger, the activity of the strain is higher and the resistance to miscellaneous bacteria is stronger. Amplified into a liquid strain, the hypha in the liquid culture medium per unit is higher in content and is not easy to laminate after long quiescent time, with high activity, excellent distribution and convenient use. The liquid strain has fast growth of bacterial colony, short color changing and shortened production period after inoculating to the production culture medium.

Description

technical field [0001] The invention relates to a method for cultivating solid strains of Cordyceps militaris. Background technique [0002] Cordyceps is a rare Chinese herbal medicine with high medicinal and tonic effects. Wild Cordyceps resources are limited, excessive exploitation will damage the environment. The active ingredients of artificially cultivated Cordyceps militaris are similar to those of wild Cordyceps, and some components even exceed wild Cordyceps. Now the method of artificially cultivating Cordyceps militaris is generally used to obtain Cordyceps militaris. [0003] In the artificial cultivation of Cordyceps militaris, strains with excellent performance are the guarantee of high yield and high quality of Cordyceps militaris. Therefore, the cultivation of solid strains is a crucial link in the artificial cultivation of Cordyceps militaris. The commonly used method for cultivating solid strains is: after the inoculation is completed, under constant te...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14
Inventor 吕尚廉郑杰辉项坤
Owner HESHAN ZHONGCHUN BIOTECH
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