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High-arsenic-resistance thiocyanide degrading strain and application thereof

A technology of thiocyanide and bacterial strains, applied in the direction of bacteria, microorganisms, water pollutants, etc., can solve the problems of hindering the utilization of iodine element, human poisoning, etc., and achieve good degradation effect, low cost and good application effect

Active Publication Date: 2021-06-18
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Long-term exposure to thiocyanate will hinder the body’s utilization of iodine and inhibit thyroid activity. 500mg NaSCN / kg body weight is the minimum lethal dose for animals. If excessive intake of thiocyanate will cause human poisoning, when the blood SCN - It is quite dangerous when the content reaches 150mg / L

Method used

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  • High-arsenic-resistance thiocyanide degrading strain and application thereof
  • High-arsenic-resistance thiocyanide degrading strain and application thereof
  • High-arsenic-resistance thiocyanide degrading strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Isolation and Identification of Pseudomonas putida Strains

[0036] (1) Separation and purification

[0037] The inventor isolated a number of bacterial strains capable of tolerating arsenic and thiocyanide from domestic sewage in Yanghu Wetland, Changsha City, Hunan Province, separated and purified them, and finally screened and obtained strains with high ability to degrade thiocyanide. The specific operation process is as follows:

[0038] P1. Typical eutrophic activated sludge was collected from domestic sewage pond in Yanghu Wetland, Changsha City, Hunan Province;

[0039] P2. Configure LB liquid enrichment medium, add sludge to LB medium at a mass ratio of 10%, enrich and cultivate in a constant temperature shaker at 150r / min at 30°C for 3 days to obtain enriched bacterial solution;

[0040] P3. Continue to inoculate the above enriched bacterial solution in fresh LB medium according to the mass ratio of 10%, and repeat the operation of P2;

[0041] P4,...

Embodiment 2

[0056] The preparation of embodiment 2 microbial bacterial agents

[0057] (1) Activation of strains: transfer the strains on the slant of the test tube stored on the nutrient agar medium at 4°C to 25°C at room temperature and activate for 4 hours;

[0058] (2) Liquid seed preparation: On the aseptic operating table, use 10 mL of sterilized distilled water to make a bacterial cell suspension through the activated test tube slant strain, and rinse it into a conical flask equipped with sterilized liquid seed medium , one test tube inoculates one Erlenmeyer flask, the pH value is natural, and liquid seeds are obtained;

[0059] (3) Fermentation process: get the liquid seed that step (2) makes and inoculate in the sterilized fermentation medium with the inoculum size of 5% (v / v), liquid fermentation preparation can obtain the bacterium that contains Pseudomonas bacterial strain agent.

[0060] Wherein, the determination of bacterial strain Pseudomonas puerpera condition is as fo...

Embodiment 3

[0068] Embodiment 3 measures the impact of the thiocyanide of different concentrations on degradation efficiency

[0069] Prepare ion medium containing 345mg / L, 642mg / L, 836mg / L, 1030mg / L, 1240mg / L, and 1468mg / L thiocyanide respectively. Except for thiocyanide, the specific formula of other components is as follows: each liter contains K H 2 PO 4 1.0g, K 2 HPO 4 1.0g, NaCl 0.1g, MgSO 4 ·7H 2 O 0.1g, FeSO 4 ·7H 2 O 0.04g, anhydrous CaCl 2 0.005g, glucose / SCN - =15(w / w), 2mL trace elements, dilute to 1L with deionized water, pH 7.7, cultivate in a constant temperature shaker at 30°C; wherein, the trace element formula is: CuCl 2 40mg, KI 100mg, MnCl 2 ·H 2 O 400mg, ZnCl 2 ·7H 2 O 400mg, HBO 3 500mg, NaMoO 4 2H 2 O 195mg, dilute to 200mL with deionized water.

[0070] The bacterial agent addition that obtains among the embodiment 2 is 2% (w / w), and the residual rhodanide concentration in the sample survey solution in a certain period of time, the assay metho...

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Abstract

The invention discloses a high-arsenic-resistance thiocyanide degradation strain and application thereof. The strain is identified as Pseudomonas putida, the number is TDB-1, the strain is preserved in the General Microbiological Center of China Committee for Culture Collection of Microorganisms, and the preservation number is CGMCC NO.21602. The strain has the characteristic of high arsenic resistance, a microbial agent prepared from the strain can effectively degrade thiocyanide in a high-concentration arsenic environment, the degradation speed is high, and the degradation effect is good.

Description

technical field [0001] The invention relates to the field of microorganisms and thiocyanide degradation, and more specifically relates to a strain highly resistant to arsenic thiocyanide degradation and application thereof. Background technique [0002] Thiocyanide is a compound containing S-C≡N, and its toxicity is about 1 / 7 of that of cyanide. In gold ore cyanidation heap leaching sites, it is derived from the conversion of cyanide, which is a derivative of cyanide, and the conversion rate can be as high as 50% or more in reducing environments such as sulfide ores. Long-term exposure to thiocyanate will hinder the body’s utilization of iodine and inhibit thyroid activity. 500mg NaSCN / kg body weight is the minimum lethal dose for animals. If excessive intake of thiocyanate will cause human poisoning, when the blood SCN - It is quite dangerous when the content reaches 150mg / L. In addition, thiocyanate can also be converted into cyanide under certain conditions. In the ac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C02F101/18C12R1/40
CPCC12N1/20C02F3/34C02F2101/18Y02P10/20
Inventor 廖骐贺立旭唐崇俭唐溪张钦雅曹维杨志辉杨卫春李青竹王海鹰
Owner CENT SOUTH UNIV
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