The invention belongs to the technical field of traditional Chinese medicine, and relates to a pharmaceutical composition for treating female climacteric syndrome and a preparation method thereof. The pharmaceutical composition is prepared from the following raw materials: folium artemisia argyi extract, corydalis tuber, Chinese angelica, toosendan fruit, combined spicebush, cortex moutan, malt, mulberry, cassia bark, green tangerine peel, rheum officinale, white peony root, curcuma rhizome, oyster, dry ginger, poria cocos, lily, fleece-flower root, mussel, and licorice. The pharmaceutical composition has the functions of tonifying qi, activating blood, regulating menstruation, relieving pains, nourishing yin, moistening lung, clear away heart fire, soothing the nerves, invigorating the spleen, and boosting the kidney, also has the advantages of good curative effect, little side effect, and user-friendliness, and is capable of coordinating the intestines and stomach.