Dosimetric smoke determination method used in full-smoke exposure experiment

Abstract

A dosimetric smoke determination method used in a full-smoke exposure experiment is used for real-time and quantitative determination of smoke dosage in a full-smoke exposure bin through combination of a quartz micro balance wafer and an inserted cell culture dish. The method has the maximum characteristic that an actual microenvironment for smoke exposure is simulated, the actual concentration (including the mass concentration of smoke particulate matter and nicotine concentration) of cigarette smoke in the full-smoke exposure bin is detected in a real-time, sensitive and quantitative mode, the dosage-effect relation of a smoke toxicity effect is accurately represented, and a real smoke dosage basis is provided for evaluation of cigarette smoke in-vitro toxicology. By applying method, the toxicity mechanism of the cigarette smoke can be better interpreted, and a method basis and technical support are provided for health risk assessment and research of tobacco products. In addition, the method is wide in applicability and can be applied to in-vitro toxicity testing and research of cigarettes, electronic cigarettes, cigarettes non-combustible in heating and aerosols different in source, and testing results are good in repeatability, high in stability and strong in sensitivity.

Description

technical field [0001] The invention relates to in vitro toxicological research on cigarette smoke, in particular to a method for measuring smoke dosimetry under full smoke exposure experiments. Background technique [0002] The in vitro toxicological study of cigarette smoke based on whole smoke exposure is one of the hotspots in the in vitro toxicity evaluation of tobacco smoke in recent years. The whole smoke exposure experiment can simulate the in vivo microenvironment of smoke exposure, and more comprehensively and truly reflect the biological effects of cigarette smoke. Toxicology studies should be based on accurate test doses. Real-time monitoring and precise control of smoke doses are an important factor affecting the reliability of the results of full smoke exposure experiments. At present, when conducting full-smoke exposure experiments, it is difficult to measure the actual smoke concentration in the exposure chamber. Usually, indirect characterization methods ar...

AI Technical Summary

Problems solved by technology

Although the characterization methods of these smoke doses can achieve the purpose of comparing the toxicity differences between test samples, they do not know the real smoke exposure concentration under actual conditions.

Images