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Application of Flt-3 Ligand in serving as biological indicator for quickly estimating ionization radiation dosage

A technology of ionizing radiation and biological indicators, which is applied in the field of ionizing radiation biological dose estimation, can solve the problems of complex sampling and long detection cycle, and achieve the effect of simple detection operation, short detection time and simple detection method

Inactive Publication Date: 2014-03-26
NAVY MEDICINE RES INST OF PLA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In recent years, with the rapid development of cell and molecular biology, some progress has been made in the research on ionizing radiation biological dosimeters at home and abroad, including chromosomal aberrations, lymphocyte micronuclei, precocious condensed chromosomes, and fluorescence in situ hybridization. However, the traditional biological dosimeter analysis method is relatively complicated to sample, requires aseptic operation, requires cell culture, and has a long detection period. , usually takes 2 to 3 days, in practical application, there are certain limitations

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  • Application of Flt-3 Ligand in serving as biological indicator for quickly estimating ionization radiation dosage
  • Application of Flt-3 Ligand in serving as biological indicator for quickly estimating ionization radiation dosage
  • Application of Flt-3 Ligand in serving as biological indicator for quickly estimating ionization radiation dosage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Implementation Example 1: Establishment of Mouse Irradiation Model and Sample Collection

[0030] Experimental animals: male C57BL / 6 mice, 7 weeks old, weighing 19±1g, purchased from Shanghai Slack Experimental Animal Co., Ltd., animal quality certificate number: 2007000534826, experimental animal production license number SCXK (Shanghai) 2007- 0005.

[0031] Serum preparation: Mice were irradiated at 60 In the cobalt source room, 80 mice were randomly divided into four groups, 20 in each group: 1 group in the control group and 4 groups in the radiation group. The mice in the radiation group received 1Gy, 3Gy, 5Gy and 7Gy total body irradiation, respectively, with a dose rate of 0.78Gy / min. 24h, 48h, and 72h after irradiation, blood was collected from the mice through the eyeball. The whole blood was clotted at 37°C for 1h, centrifuged at 3000rpm×15min, and the supernatant serum was separated and distributed in centrifuge tubes at 100μl / tube. Aliquoted serum was froz...

Embodiment 2

[0032] Implementation Example 2: Dose-effect relationship of different doses of ionizing radiation on mouse serum Flt-3L concentration

[0033] (1) Detection of Flt-3L content in serum of non-irradiated mice

[0034] Testing materials and methods:

[0035] Cytokine antibody chip detection. The chip name is: Custom Mouse Array chip, customized by RayBiotech. The chip detection operation steps are as follows:

[0036] ① Place the chip bag at room temperature for 30 minutes, tear off the sealing film, mark the chip, and dry it for 2 hours on a clean bench;

[0037] ②Sample preparation: 60μl serum + 60μl diluent, mix well;

[0038] ③Preparation of standard substance: take 10000pg / ml standard solution (std1), three-fold dilution 6 times (std2~7), the lowest concentration is 13.7pg / ml (std7).

[0039] ④ Preparation of negative control: Take 120 μl of diluent (CNTRL)

[0040] ⑤ Chip blocking and sample incubation: add 100 μl blocking solution to each well, block at room tem...

Embodiment 3

[0063] Implementation Example 3: Statistical Analysis and Fitting of Surfaces and Formulas

[0064] It can be seen from Table 1-4 that the sensitivity of Flt-3L to radiation increases with time after irradiation, and the sensitivity increases, that is, with the same radiation dose, the degree of increase in the concentration of Flt-3L increases with time, and statistics In the analysis, the irradiation time and irradiation dose were used as the influencing factors, and the Flt-3L concentration was used as the effect. SASS9.3 was used to analyze the secondary corresponding surface analysis, and the secondary interaction between irradiation time and irradiation dose was analyzed, and the time- The dose-response corresponding surface diagram, the fitting time-dose-response curve is response=191.54-2.65*time-52.58*dose+0.027*time 2 +1.04*dose 2 +3.06*time*dose, R 2 =0.9711, where response is the concentration of Flt-3L, time is the time after irradiation, and dose is the irradia...

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Abstract

The invention belongs to a biological indicator for quickly estimating the ionization radiation dosage and in particular relates to application of Flt-3 Ligand in serving as the biological indicator for quickly estimating the ionization radiation dosage. After irradiation is executed, the expression quantity of Flt-3L in serum or plasma, the after-irradiation time and the radiation dosage have a time-dosage-effect relation; response curve surfaces and reduction formulas of all the parameters (the expression quantity of the Flt-3L, the after-irradiation time and the radiation dosage) are fit through statistic analysis; therefore, the radiation dosage is estimated according to the response curve surfaces and the reduction formulas under the condition that the expression quantity of the Flt-3L and the after-irradiation time are known; moreover, the expression quantity of the Flt-3L can be quickly detected by a simple feasible method such as ELISA (enzyme-linked immuno sorbent assay) and a protein chip; the number of samples for detection is small, sampling and storage are convenient, the time is short, and the result is objective, so that the Flt-3 Ligand can serve as the biological indicator for quickly estimating the ionization radiation dosage.

Description

1 technical field [0001] The invention belongs to the field of ionizing radiation biological dose estimation. It specifically relates to the application of Flt-3Ligand as a biological indicator for estimating ionizing radiation dose. 2 background technology [0002] With the rapid development of science and technology, radioactive sources and radiation technology are widely used in the fields of industry and agriculture, medicine, teaching and even military affairs. However, while nuclear technology benefits mankind, it also poses a potential threat to public safety and public health. Acute radiation sickness is the epitome of this threat. Acute radiation sickness is a deterministic effect of ionizing radiation injury, and it is also the most serious radiation biological effect, which is characterized by the increase of damage with the increase of radiation dose. However, there is a false recovery period for bone marrow type radiation sickness above moderate (>2Gy). If...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6803G01N2800/40G01T1/02
Inventor 刘琼何颖沈先荣蒋定文陈伟侯登勇刘玉明王庆蓉钱甜甜李珂娴
Owner NAVY MEDICINE RES INST OF PLA
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